Tremella fuciformis strain and application thereof in tremella polysaccharide production

A Tremella fungus strain and tremella polysaccharide technology are applied in applications, fungal products, microorganisms, etc. It can solve the problems of single molecular size of polysaccharides, high protein impurity content, single molecular weight of products, etc., and achieve easy control conditions, easy extraction, and The effect of a short growth cycle

Active Publication Date: 2020-01-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the fermentation method has many problems such as low polysaccharide yield, high protein impurity content, single molecular size of the output polysaccharide, and high input-output ratio.
Therefore, it is imperative to screen and obtain a high-yield strain for fermenting tremella polysaccharides, and to solve the problems of high input cost and single molecular weight of the breakthrough product.

Method used

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  • Tremella fuciformis strain and application thereof in tremella polysaccharide production
  • Tremella fuciformis strain and application thereof in tremella polysaccharide production
  • Tremella fuciformis strain and application thereof in tremella polysaccharide production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] figure 1 As shown in (a), the Tremella fungus strain CGMCC No.17675 was smeared in PDA solid medium (glucose agar medium), and cultured at a constant temperature of 26°C for 5 days to obtain a single colony (such as figure 1 Shown in (b)) and its 18s sequence was sequenced, and after Blast comparison, it was determined to be a Tremella fungus strain. The 18s sequence is shown in the sequence listing SEQ ID No.1.

Embodiment 2

[0059] Embodiment 2: the preparation of fermented liquid

[0060] The preparation method of a single colony is the same as in Example 1.

[0061] Solid-state fermentation culture of high-yield strains: Pick the obtained strains into the seed medium (glucose 25g / L, soybean peptone 2g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.46g / L, K 2 HPO 4 1 g / L, pH 6.0), 26°C, 170rpm constant temperature culture for 3 days. Inoculate in solid medium (glucose 15g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, bran 8g, soybean meal 12g, relative humidity 80±5%), cultured at a constant temperature of 26°C for 6-8 days. The yield of Tremella polysaccharides was 12.9g / L as detected by the sulfate anthrone method, of which the large molecular weight was 1000-1500kDa, accounting for 54% of the total polysaccharides, and the small molecular weight was 100-300kDa, accounting for 46% of the total polysaccharides.

Embodiment 3

[0062] Embodiment 3: the preparation of fermented liquid

[0063] The preparation method of a single colony is the same as in Example 1.

[0064] Solid-state fermentation culture of high-yield strains: Pick the obtained strains into the seed medium (glucose 25g / L, soybean peptone 2g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.46g / L, K 2 HPO 41 g / L, pH 6.0), 26°C, 170rpm constant temperature culture for 3 days. Inoculate in solid medium (glucose 15g / L, (NH 4 ) 2 SO 4 3g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, bran 8g, soybean meal 12g, relative humidity 80±5%), cultured at a constant temperature of 26°C for 6-8 days. The yield of tremella polysaccharides is 22.5g / L as detected by the sulfate anthrone method, of which the large molecular weight is 1000-1500kDa, accounting for 75% of the total polysaccharides, and the small molecular weight is 100-300kDa, accounting for 25% of the total polysaccharides.

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Abstract

The invention discloses a tremella fuciformis strain which is screened out. The tremella fuciformis strain is named as XY and is preserved in China General Microbiological Culture Collection Center, apreservation date is April 25, 2019, and a preservation number is CGMCC No.17675. The invention further provides application of the tremella fuciformis strain in tremella polysaccharide production. Amain process of using the tremella fuciformis strain for producing tremella polysaccharides includes the steps: a seed solution prepared from CGMCC No.17675 is used for inoculating a solid-state fermentation culture medium, and fermentation is conducted to obtain a fermentation broth. High-molecular-weight polysaccharides with the molecular weight of 1000-1500 kDa and low-molecular-weight polysaccharides with the molecular weight of 100-300 kDa can be prepared, a total yield can achieve 15-25 g / L, the protein content is smaller than 0.3%, compared with a liquid-state fermentation method, theprocess of using the tremella fuciformis strain for producing the tremella polysaccharides has significant cost advantages, and therefore the tremella fuciformis strain has good application prospectsin industrial production of the tremella polysaccharides.

Description

technical field [0001] The invention belongs to the field of microorganisms and fermentation engineering, and relates to a tremella fungus strain and its application in the production of tremella polysaccharides. Background technique [0002] Tremella, also known as white fungus, belongs to the subclass Tremellaceae, which has high nutritional value and medicinal efficacy. Tremella, as a special product of my country, is planted in many provinces such as Zhejiang, Fujian, and Hubei. As the main production area of ​​tremella, Fujian Province has reached more than 90% of the national total output. Tremella contains carbohydrates, proteins, vitamins and a variety of amino acids, and the most important biologically active substance is Tremella polysaccharide, which can be obtained by direct extraction or fermentation from Tremella fruiting bodies. It has immune regulation, anti-tumor, Anti-oxidative aging, lowering blood sugar and blood lipids, anti-coagulation and thrombus, an...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12P19/04C12R1/645
CPCC12N1/14A01H15/00C12P19/04C12R2001/645C12N1/145
Inventor 李莎葛辛燕徐虹雷鹏续晓琪
Owner NANJING UNIV OF TECH
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