The invention relates to a
quick test method. Firstly, rice
bran is partially degreased, lipoxidase in the rice
bran which is partially degreased is extracted with a
buffer solution, the lipoxidase extracting solution is added to a prepared
phosphate-
linoleic acid-
polysorbate 20 substrate solution, changes of light-absorption photometric values are tested under 234 nm in 5 minutes, meanwhile, the
protein content in the lipoxidase extracting solution is tested, and then activity of the lipoxidase in the rice
bran can be obtained. According to the method, the activity of the lipoxidase in the rice bran is effectively activated through partial
degreasing, the
detection limit is raised, the
buffer solution, the substrate solution and passivating crude
enzyme serve as reference solutions, test errors of the crude
enzyme are effectively eliminated, and complicated steps of passivating of the crude
enzyme are eliminated. The method is easy and convenient to operate, precise in result, good in reproducibility, capable of being widely used for grain and
oil quality test and control in grain
processing enterprises, storage enterprises and grain and oil test organizations.