A detection kit for s100 protein

A technology for detecting kits and proteins, which is applied in the field of medical testing, can solve problems such as insufficient linear range, and achieve the effects of shortening detection time, high sensitivity and specificity, and improving sensitivity

Active Publication Date: 2022-03-01
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the above-mentioned existing products and patented products generally have the problem that the linear range is not wide enough

Method used

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  • A detection kit for s100 protein
  • A detection kit for s100 protein
  • A detection kit for s100 protein

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0062] The preparation method of kit of the present invention is:

[0063] 1. Preparation of calibrator: Dissolve the S100 antigen in the diluent of the calibrator according to a certain concentration, and prepare the concentrations as 0ng / mL, 0.5ng / mL, 2ng / mL, 10ng / mL, 50ng / mL, 200ng / mL mL of calibrator solution, respectively recorded as S0, S1, S2, S3, S4, S5.

[0064] 2. Preparation of magnetic particle suspension: select the appropriate magnetic particle stock solution, coat the S100 antibody on the surface of the magnetic particle at a concentration of 0.1-1.0 μg / T, and prepare the S100 suspension for use.

[0065] 3. Prepare sample dilution: add 0.1% to 10% (w / v) chelating agent, 1‰ (w / v) preservative, 2% (w / v) to the Bis-Tris buffer system with pH 7.4 Protein stabilizer, 1‰ (w / v) dispersant, 1‰ (w / v) surfactant and 10-300μg / mL blocking agent were prepared as sample diluent.

[0066] 4. Preparation of enzyme conjugates: Add 1‰ (w / v) preservative, 1‰ (w / v) surfactant an...

Embodiment 1

[0072] Example 1 Preparation of S100 protein detection kit for melanoma diagnosis

[0073] 1. Kit preparation

[0074] 1. Prepare blocking solution

[0075] The blocking solution is prepared from 0.1M Tris buffer with pH 7.2, which contains 1‰ (w / v) preservative Proclin150, 2% (w / v) protein stabilizer hormone-free human serum, 1 % (w / v) of sucrose and 5% (w / v) of glycerol.

[0076] 2. Prepare enzyme conjugate dilution

[0077] Enzyme conjugate diluent is prepared by 0.05M pH 7.4 Bis-Tris buffer, which contains 1‰ (w / v) preservative Proclin150, 1‰ (w / v) surfactant Tween 20, 2% (w / v) protein stabilizer dehormone human serum and 0.5‰ (w / v) inert carmine pigment.

[0078] 3. Prepare sample diluent

[0079] The sample diluent is prepared by 0.05M pH 7.4 Bis-Tris buffer, which contains 5% (w / v) chelating agent EGTA, 1‰ (w / v) preservative Proclin150, 2% (w / v) / v) protein stabilizer dehormone human serum, 1‰ (w / v) dispersant sodium dodecyl sulfonate, 1‰ (w / v) surfactant Tween 20...

Embodiment 2

[0098] Embodiment 2 Test kit precision of the present invention

[0099] Three batches of kits were taken for precision experiments, and Q1, Q2, and Q3 were measured 20 times to calculate the variation in concentration. The results of the intra-analysis precision and inter-batch precision of the kits are shown in Table 1 and Table 2.

[0100] Table 1 Intra-analytical precision of kits

[0101]

[0102]

[0103] Table 2 Precision between batches of kits

[0104]

[0105]

[0106] It can be seen from the data in Table 1 that the coefficients of variation within the analysis of the three batches of low, medium and high concentration quality control products of the three batches of kits are all less than 6%. The batch-to-batch variation of control products is all less than 5%, indicating that the precision of the kit of the present invention is better.

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Abstract

The invention relates to the technical field of medical testing, in particular to a detection kit for S100 protein. The S100 protein detection kit includes magnetic particle suspensions coated with S100 antibodies, sample diluents, enzyme conjugates, calibrators and quality controls; sample diluents contain chelating agents, preservatives, and protein stabilizers , dispersant, surfactant, Bis-Tris buffer of blocking agent; chelating agent is EGTA, blocking agent is one of tumor cleaning antibody 1, tumor cleaning antibody 2, tumor cleaning antibody 3 or tumor cleaning antibody 4 or a mixture of two or more. The S100 protein detection kit has a wide linear range, high sensitivity and specificity, and realizes rapid and automatic detection of the S100 protein.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a detection kit for S100 protein. Background technique [0002] Malignant melanoma (melanoma) is a relatively common malignant tumor of the skin, mucous membrane and pigmented membrane in clinical practice, and it is also one of the malignant tumors with the fastest growing incidence rate, with an annual growth rate of 3% to 5%. In 2018, there were 287,723 new cases of melanoma worldwide (accounting for 1.6%), and the number of deaths was 60,712 (accounting for 0.6%). According to statistics, in 2015, there were 8,000 new cases of melanoma in my country (accounting for 0.2%), and the number of deaths was 3,200 (accounting for 0.1%). Although melanoma is a rare malignant tumor in my country, its mortality rate is high and its incidence is increasing year by year. Therefore, melanoma has become one of the diseases that seriously endanger the health of our people. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/57484G01N33/5743G01N33/54326
Inventor 刘雅奇史小芹渠文涛周金龙万鹏付光宇吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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