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Preparation process of microbial agent based on directional screening of microorganisms

A technology of microbial inoculum and preparation process, applied in the field of fermentation, can solve the problems of waste of resources, secondary environment, pollution, low secondary utilization efficiency, etc.

Pending Publication Date: 2020-01-14
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the dry powder of antibiotic bacteria residue in China is more than 2 million tons per year, and the secondary utilization efficiency is low, resulting in waste of resources and secondary environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1) Take long-term natural fermentation samples from the fungus slag pond in the production workshop, and take fresh chicken manure or pig manure samples from the farm. The sample must be pretreated. Before the LB medium is diluted and coated, the sample is diluted 10 times, and boiled in an EP tube for 10 minutes. Before the PDA medium is diluted and coated, 25ug / ml ampicillin is added after the sample is diluted 10 times. base for ampicillin-resistant plates. After the samples were pretreated, the plates were coated with LB medium or PDA-resistant medium, and cultured in an incubator at 37±0.5°C for 24h.

[0052] Pick a single colony from the coated plate, use LB medium or PDA-resistant medium, carry out two consecutive streaks for pure culture, and culture at 37±0.5°C for 24h. After the screened purified plate or slant strains are identified through colony morphology identification and microscopic examination, they are respectively numbered and marked as the parent s...

Embodiment 2

[0064]1) Take long-term natural fermentation samples from the fungus slag pond in the production workshop, and take fresh chicken manure or pig manure samples from the farm. The sample must be pretreated. Before the LB medium is diluted and coated, the sample is diluted 10 times, and boiled in an EP tube for 10 minutes. base for ampicillin-resistant plates. After the samples were pretreated, the plates were coated with LB medium or PDA-resistant medium, and cultured in an incubator at 37±0.5°C for 12h.

[0065] Pick a single colony from the coated plate, use LB medium or PDA-resistant medium, carry out three consecutive streaks for pure culture, and culture at 37±0.5°C for 24h. After the screened purified plate or slant strains are identified through colony morphology identification and microscopic examination, they are respectively numbered and marked as the parent stock.

[0066] 2) Scrape a ring from the isolated yeast and bacillus purification plates, inoculate them into...

Embodiment 3

[0077] 1) Take long-term natural fermentation samples from the fungus slag pond in the production workshop, and take fresh chicken manure or pig manure samples from the farm. The sample must be pretreated. Before the LB medium is diluted and coated, the sample is diluted 10 times, and boiled in an EP tube for 10 minutes. Before the PDA medium is diluted and coated, 40 ampicillin is added after the sample is diluted 10 times. The PDA medium is Ampicillin resistant plates. After the samples were pretreated, the plates were coated with LB medium or PDA-resistant medium, and cultured in an incubator at 37±0.5°C for 20 hours.

[0078] Pick a single colony from the coated plate, use LB medium or PDA-resistant medium, perform 2-3 consecutive streaks for pure culture, and culture at 37±0.5°C for 24 hours. After the screened purified plate or slant strains are identified through colony morphology identification and microscopic examination, they are respectively numbered and marked as ...

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Abstract

The invention relates to a preparation process of a microbial agent based on directional screening of microorganisms. According to the invention, various screened application microorganisms are utilized, steps of acid protease enzymolysis, two-stage natural variable-temperature fermentation and multi-gradient pH solid-state mixed fermentation are carried out on mushroom dregs, kasugamycin and polyoxin residual liquid concentrate generated in the kasugamycin and polyoxin production process, a microbial agent with the biological activity is obtained, and peculiar smell residues in mushroom dregsand kasugamycin and polyoxin residual liquid concentrates are completely removed. The total number of effective viable bacteria in each gram of active material in the microbial agent is more than 1 billion; the crude protein of the product accounts for 65-75% of the total dry matter; the downstream treatment difficulty of mushroom dreg and kasugamycin and polyoxin residual liquid concentrate in the production process of kasugamycin and polyoxin is solved, the economic value of the mushroom dreg as production solid waste is improved, and the produced mixed microbial agent has application prospects in multiple directions of organic fertilizers, feeds and the like.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a preparation process of a microbial bacterial agent based on directional screening of microorganisms, more specifically to a directional screening method of natural bacterial strains, and the preparation by solid-state mixed fermentation using antibiotic residues as raw materials Methods of microbial inoculation. Background technique [0002] Microbial agent is a low-carbon, pure natural, harmless, and non-polluting biological raw material. It belongs to the green industry that turns waste into treasure. It increases the added value of bacterial residues and uses cutting-edge technology of technological innovation to increase the production efficiency of production enterprises. Further enhance the sustainable development of pharmaceutical enterprises. [0003] At present, the price of domestic agricultural products is low, and the production and supply of dome...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/07
CPCC12N1/20C12N1/16
Inventor 马文艳王卫富潘忠成李蒲民
Owner 陕西麦可罗生物科技有限公司
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