Method for detecting antibiotic residues in water body environment based on solid phase extraction technology and liquid chromatography tandem mass spectrometry technology

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of synchronous detection of multiple types of multi-component antibiotics, can solve the problems of large amount of water samples, high detection limit, and single species, so as to reduce the signal-to-noise ratio and improve the peak value. Shape, reduce the effect of preprocessing time

Pending Publication Date: 2020-01-24
ZHEJIANG UNIV OF TECH +1
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Problems solved by technology

Existing detection methods for veterinary drug antibiotic residues in water samples have one or more of the following disadvantages, such as: the number and type of antibiotics are small and single, the pretreatment process is complicated, the amount of water samples to be tested is large, and the detection limit is high, etc.

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  • Method for detecting antibiotic residues in water body environment based on solid phase extraction technology and liquid chromatography tandem mass spectrometry technology
  • Method for detecting antibiotic residues in water body environment based on solid phase extraction technology and liquid chromatography tandem mass spectrometry technology
  • Method for detecting antibiotic residues in water body environment based on solid phase extraction technology and liquid chromatography tandem mass spectrometry technology

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Embodiment 1

[0030] A method based on solid-phase extraction and liquid chromatography-tandem mass spectrometry for the detection of 42 antibiotics in the water environment. Specific antibiotics include fluoroquinolones: marbofloxacin, fleroxacin, pefloxacin, ciprofloxacin, and danfluxacin Star, enrofloxacin, orbifloxacin, difloxacin, sarafloxacin, sparfloxacin, cinoxacin, flumequine; sulfonamides: sulfacetamide, sulfadiazine, sulfapyridine, sulfamethyl Pyrimidine, sulfamethoxazole, sulfamethiadiazole, sulfamethazine, sulfamethoxypyridazine, sulfachloropyridazine, sulfamethoxazole, sulfamethoxine, sulfamethoxine, sulfamethoxine Oxazole, benzoyl sulfonamide, sulfabenzopyrazole, sulfadimethoxine, sulfaquinoxaline; macrolides: spiramycin, tilmicosin, oleandomycin, tylosin, Beilimycin, erythromycin, josamycin, roxithromycin; chloramphenicol: chloramphenicol, thiamphenicol, florfenicol; lincosamides: lincomycin, clindamycin white.

[0031] The method comprises the steps of:

[0032] (1) Samp...

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Abstract

The invention provides a method for detecting antibiotic residues in a water body environment based on the solid phase extraction technology and the liquid chromatography tandem mass spectrometry technology. In terms of detection effect, the signal-to-noise ratio is improved by optimizing the mass spectrum conditions, the peak shape is improved, and a lower detection limit of the method of a sample reaches 0.08 to 9.37ng / L; in terms of sample pretreatment, only 50mL water sample is required, thereby shortening the pretreatment time; in terms of the number of antibiotics and the detection time,42 kinds of antibiotics in 5 major categories, such as fluoroquinolones, sulfonamides, macrolides, chloramphenicols and lincomamides, can be separated and detected at one time just within 12min by optimizing the chromatographic conditions.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection, in particular to a method for synchronous detection of multi-type and multi-component antibiotics in a water body environment. Background technique [0002] Antibiotics are a commonly used class of veterinary drugs, which are mainly used to prevent and treat diseases and promote growth, but most of the antibiotics administered to animals are directly excreted without being metabolized (Analytical Laboratory, 2018, 37, 103-107). These antibiotics discharged into the natural environment can seriously affect the ecosystem. Kaoru Eguchi et al. (Chemosphere, 2004, 57, 1733–1738) studied the effects of antibacterial drugs commonly used as veterinary drugs on the growth of green algae, and the results showed that these veterinary drugs showed strong growth inhibitory activity on green algae. The long-term abuse of antibiotics has also led to the emergence of drug-resistant bacteria. In 2...

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Application Information

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IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/72G01N2030/062
Inventor 孙楠薛大海吴俐勤
Owner ZHEJIANG UNIV OF TECH
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