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Gallium-contained polycaprolactone/bioglass porous bone repair 3D printing support and application thereof in infectious bone defect repair

A technology of bioglass and polycaprolactone, which is applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of soft tissue damage, easy adhesion, and high risk of bacterial exposure, so as to promote osteogenic differentiation and inhibit osteoclast differentiation , the effect of uniform distribution of pores

Active Publication Date: 2020-02-04
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the defect area, especially in the presence of exogenous bone filling materials, there is a high risk of bacterial exposure and easy adhesion
In addition, due to the serious damage to the local soft tissue of the trauma, the ability of the local autoimmune system to eliminate bacteria is reduced, which further increases the difficulty of the treatment of infectious bone defects.
When severe infection exists, a second operation is often required to remove the infected bone filling material, thoroughly debride the wound and eliminate the infection focus, and then long-term high-dose antibiotic treatment will bring serious pain and economic burden to the patient

Method used

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  • Gallium-contained polycaprolactone/bioglass porous bone repair 3D printing support and application thereof in infectious bone defect repair
  • Gallium-contained polycaprolactone/bioglass porous bone repair 3D printing support and application thereof in infectious bone defect repair
  • Gallium-contained polycaprolactone/bioglass porous bone repair 3D printing support and application thereof in infectious bone defect repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 3D printing and material science characterization of embodiment 1 bracket

[0038] 1.1 Fully stir and mix the prepared bioglass powder containing 8% gallium with PCL dissolved in dichloromethane respectively to make a paste suitable for printing and put it into the barrel, wherein the bioglass powder containing gallium and The mass ratio of PCL is 3:7. Set a circular model with a diameter of 10mm and a height of 2mm on the 3D bioprinting of ENVISION TEC, and set the height of each layer to 0.32mm. Set the printing speed to 3mm / s, the printing temperature to 25°C, and the extrusion pressure to 2bar. After the printed stent is dried, it is sprayed with gold, and the scanning electron microscope is taken and analyzed by EDS.

[0039] figure 1 It is the result figure of the scanning electron microscope in embodiment 1.1, figure 1 It was shown that the surface of the stent containing bioglass was rougher than that of the pure PCL stent.

[0040] 1.2 Print a stent with a...

Embodiment 2

[0042] Example 2 Evaluation of the antibacterial effect of gallium-containing polycaprolactone / bioglass 3D printing scaffold on methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E.coli)

[0043] 2.1 After ultrasonic vibration, the number of bacteria adhered to the surface of the scaffold was quantified by gradient dilution plate counting, which proved the ability of the scaffold to inhibit bacterial proliferation. Place the sterilized scaffold in a 24-well plate, add 1mL of 1*10^ 6 CFUs / mL of E.coli and MRSA bacteria solution, after incubating in a 37-degree incubator for 24 hours, take out the inner bracket of the orifice plate in the ultra-clean bench, gently rinse it in PBS three times, put it into a sterile centrifuge tube, Add 2 mL of LPBS to completely immerse the scaffold in the liquid, and ultrasonically shake for 15 minutes. The resulting bacterial suspension is plated and counted by gradient dilution. After incubation in the incubator for 24 ho...

Embodiment 3

[0047] Example 3 evaluates the promotion effect of gallium-containing polycaprolactone / bioglass 3D printing scaffold on the osteogenic differentiation of BMSCs and the inhibitory effect on the osteoclastic differentiation of BMM.

[0048] 3.1 The BMSCs of the primary isolation of 4-week-old male C57 mice were used as 1*10^ 5 The density of each well was planted in a 12-well plate. After the cells adhered to the wall overnight, an osteogenic induction medium and a transwell chamber with a scaffold in the upper chamber were added. The medium was changed every 3 days, and alkaline phosphatase (ALP ) staining to observe the promotion effect of 3D printed scaffolds on the osteogenic differentiation ability of BMSCs. Alizarin red staining was performed on day 21 to observe the effect of scaffolds on the late mineralization of BMSCs.

[0049] Figure 5 It is the experimental result of alkaline phosphatase in Example 3.1. It can be seen that the scaffold containing bioglass can prom...

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Abstract

The invention discloses a gallium-contained polycaprolactone / bioglass porous bone repair 3D printing support and the application thereof in infectious bone defect repair. Gallium has the good antibacterial property, and can inhibit bone damage differentiation as well, so that gallium can effectively inhibit osteolysis which might exist in bone infection. Bioglass has the capability of promoting osteogenic differentiation, and mesoporous bioglass particles have pore channel structures, and thereby being capable of being loaded with ingredients and releasing the ingredients slowly. According tothe gallium-contained polycaprolactone / bioglass porous bone repair 3D printing support and the application thereof in infectious bone defect repair, the antibacterial property and the bone damage inhibition effect of gallium, and the bone differentiation promotion effect of bioglass are combined, a local infection focus is directly removed, and the balance between osteogenesis and bone damage in the bone repair process can be further adjusted; and the functional bone repair support is built by applying the 3D printing technology and utilizing the good biocompatibility and the dynamic supporting action of polycaprolactone, the structure of the support can be flexibly designed according to the features of a damaged part, the size of the support is controllable, and the support has the significant application prospect in bone defect repair.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to gallium-containing polycaprolactone / bioglass porous bone repair 3D printing scaffold and its application in the repair of infectious bone defects. Background technique [0002] Severe trauma and resection of bone tumors can lead to large bone defects, and bone filling materials are often required for treatment. In the defect area, especially in the presence of exogenous bone filling materials, the risk of bacterial exposure is high and it is easy to adhere. Moreover, due to the serious damage of local soft tissue in the trauma, the ability of the local autoimmune system to eliminate bacteria is reduced, which further increases the difficulty of the treatment of infected bone defects. When severe infection exists, a second operation is often required to remove the infected bone filling material, thoroughly debride the wound to eliminate the infection focus, and then long-term ...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/10A61L27/56A61L27/54A61L27/02B33Y80/00
CPCA61L27/18A61L27/10A61L27/56A61L27/54A61L27/025B33Y80/00A61L2300/102A61L2300/404A61L2430/02C08L67/04
Inventor 汤亭亭王敏琪杨盛兵
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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