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Lycium barbarum polysaccharide, lycium barbarum red pigment, lycium barbarum polypeptide prepared by simultaneous separation and preparation method of lycium barbarum polysaccharide, lycium barbarum, lycium barbarum polypeptide

A technology of Lycium barbarum polysaccharide and Lycium barbarum red pigment is applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems such as the decrease of extraction rate and extraction purity, waste of raw material residues, etc., and achieves low cost and reduced Interference of impurities and the effect of improving the utilization value

Active Publication Date: 2020-02-04
百瑞源枸杞股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In summary, in the prior art, the extraction of Lycium barbarum polysaccharides, polypeptides, and Lycium barbarum is often carried out using Lycium barbarum as the starting raw material. During the extraction process, interference from other impurities will inevitably be received, resulting in a decline in the extraction rate and extraction purity. In the process of extracting effective substances from wolfberry as raw material, a lot of waste of raw material residues is often caused

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0047] Experimental example 1 Determination method of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0048] In the present invention, the determination method of Lycium barbarum polysaccharide is as follows: the determination of polysaccharide content in the sample refers to the SN / T4260-2015 method; the method of Lycium barbarum is: the determination of Lycium barbarum content in the sample is determined according to the established HPLC method; the method of Lycium barbarum polypeptide For: determined by using graphite digestion-semi-automatic nitrogen determination method.

[0049] 1. Determination of Lycium barbarum polysaccharides: using the method for the determination of reducing sugar content. Prepare a buffer solution, and the solution formula is to add 13.4 g of potassium sodium tartrate, 3,5-dinitrosalicylic acid, 1.8 g of sodium hydroxide, and 0.7 g of phenol per 100 mL of water. Use the glucose standard to draw a standard curve, ac...

Embodiment 1

[0057] Embodiment 1 Preparation of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0058] Using dried Lycium barbarum as raw materials, Lycium barbarum polysaccharides, Lycium barbarum, and Lycium barbarum active peptides were prepared according to the following process:

[0059] Using dried wolfberry as raw material, weigh 30g wolfberry and add 300ml deionized water to soak for 2 hours, crush it, ultrasonically extract it for 20 minutes, and centrifuge it at 5000g for 10 minutes. The supernatant is used to prepare wolfberry polysaccharide, and the precipitate is used to extract wolfberry red pigment and wolfberry peptides.

[0060] (1) Preparation of wolfberry polysaccharide

[0061] Add 540 ml of 90% ethanol to 270 ml of supernatant, let stand at 4° C. for 6 hours, centrifuge at 3000 g for 10 minutes, precipitate and freeze-dry to obtain 1.60 g of Lycium barbarum polysaccharide with a purity of 50.73% and an extraction rate of 5.34%.

[0062]...

Embodiment 2

[0067] The preparation of embodiment 2 Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0068] Using dried wolfberry as raw material, weigh 20g wolfberry and add 220ml of deionized water to soak for 5 hours, then pulverize, ultrasonically extract for 40 minutes, and centrifuge at 5000g for 15 minutes to obtain 200ml of supernatant for preparing wolfberry polysaccharide, and precipitate for extraction Lycium barbarum and Lycium barbarum peptides.

[0069] (1) Preparation of wolfberry polysaccharide

[0070] Add 1000ml of 80% ethanol to the supernatant, let stand at 4°C for 9 hours, centrifuge at 4000g for 15 minutes, freeze-dry the precipitate, and obtain Lycium barbarum polysaccharide with a purity of 51.67% and an extraction rate of 6.98%.

[0071] (2) Preparation of Lycium barbarum

[0072] Lyophilize 19.1 g of precipitate obtained from preliminary extraction of wolfberry, and grind again to obtain wolfberry powder. Add 324 ml of organic solv...

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Abstract

The invention discloses lycium barbarum polysaccharide, lycium barbarum red pigment and lycium barbarum polypeptide prepared by simultaneous separation and a preparation method of the lycium barbarumpolysaccharide, the lycium barbarum red pigment and the lycium barbarum polypeptide, and belongs to the technical field of deep processing of lycium barbarum. The preparation method comprises the steps that lycium barbarum is used as a raw material, the lycium barbarum polysaccharide is obtained through water-adding soaking extraction and ethanol extraction, meanwhile, organic solvent is continuously added to the lycium barbarum residue obtained by the preparation for extraction, the lycium barbarum red pigment is obtained, water and probiotics are added to the residue obtained in the previousstep, microbial fermentation is performed, and the lycium barbarum polypeptide is prepared. The problems of waste of raw materials and the like are solved, the utilization value of lycium barbarum ismaximized, efficient cooperativity of the continuous extraction of the lycium barbarum polysaccharide, the lycium barbarum red pigment and the lycium barbarum polypeptide is used, the separate extraction efficiency and purity are improved, the interference of other ingredients on the extraction of a target ingredient in the extraction process is effectively avoided, and wide application in industrial production is achieved.

Description

technical field [0001] The invention belongs to the technical field of deep processing of wolfberry, and in particular relates to a synchronous separation and preparation of wolfberry polysaccharide, wolfberry red pigment, wolfberry polypeptide and a preparation method thereof. Background technique [0002] Lycium barbarum is a plant in the family Solanaceae. Lycium barbarum fruit is a traditional medicine and food homologous medicinal material in China. Lycium barbarum has a long history of consumption in my country. The "Dictionary of Chinese Medicine" records that wolfberry fruit has the effects of nourishing the liver and kidney, benefiting energy and improving eyesight. Modern biological research shows that wolfberry has various biological activities such as anti-oxidation, blood fat reduction, anti-aging, liver protection, etc. With people's attention to health, the consumption of wolfberry is increasing year by year. In order to meet the needs of different groups of pe...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07C403/24C09B61/00C09B67/54C12P21/00C12R1/125C12R1/225C12R1/46
CPCC07C403/24C08B37/00C08B37/0003C09B61/00C09B67/0096C12P21/00C07C2601/16
Inventor 靳艳庄晶玲叶明亮张金宏郝向峰杨丽丽马奇虎姬宏礼
Owner 百瑞源枸杞股份有限公司
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