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Viscoelastic hydrogel as well as preparation method and application thereof

A hydrogel and viscoelastic technology, applied in the field of injectable hydrogel, can solve the problems of limited application of cell therapy, cell death, and reduced curative effect, and achieve the effect of cheap and easy-to-obtain raw materials, simple preparation method, and low risk factor

Active Publication Date: 2020-02-07
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during the injection process, the cells will be subjected to different degrees of shear stress, pressure, etc. at different positions of the syringe, which will lead to a large number of cell death
In addition, the few cells that can precisely localize to the defect site are also in a free state and are likely to be washed away by joint fluid, thereby reducing the efficacy
[0006] Therefore, the application of cell therapy in minimally invasive cartilage regeneration is limited due to the lack of suitable injection medium

Method used

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  • Viscoelastic hydrogel as well as preparation method and application thereof
  • Viscoelastic hydrogel as well as preparation method and application thereof
  • Viscoelastic hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of aldehyde-modified cellulose nanocrystal solution:

[0044] 1) Weigh the same mass of cellulose nanocrystals (CNC) and NaIO 4 .

[0045] 2) Take enough deionized water to dissolve CNC and NaIO 4 , adding dilute sulfuric acid (concentration 0.1mol / L) to adjust pH=3.

[0046] 3) In a water bath at a constant temperature of 40°C and protected from light while stirring, react for 3 hours.

[0047] 4) After the reaction is completed, the sample is centrifuged with deionized water, and then dialyzed for three days with a 4000 mesh dialysis bag (changing deionized water every 2h), until no IO 3 — Residue: first drop a small amount of centrifuged supernatant on the starch potassium iodide test paper, then add a small amount of dilute sulfuric acid dropwise until the test paper does not change color; titrate the dialyzed sample, and the aldehyde content is 16.27%.

[0048] 5) The sample was freeze-dried to obtain cellulose nanocrystalline solid (a-CNC) afte...

Embodiment 2

[0055] 1. Preparation of aldehyde-modified cellulose nanocrystal solution:

[0056] 1) Weigh the same mass of cellulose nanocrystals (CNC) and NaIO 4 .

[0057] 2) Take enough deionized water to dissolve CNC and NaIO 4 , adding dilute sulfuric acid (concentration 0.1mol / L) to adjust pH=3.

[0058] 3) In a water bath at a constant temperature of 40°C and protected from light while stirring, react for 3 hours.

[0059] 4) After the reaction is completed, the sample is centrifuged with deionized water, and then dialyzed for three days with a 4000 mesh dialysis bag (changing deionized water every 2h), until no IO 3 — Residue: first drop a small amount of centrifuged supernatant on the starch potassium iodide test paper, then add a small amount of dilute sulfuric acid dropwise until the test paper does not change color; titrate the dialyzed sample, and the aldehyde content is 16.27%.

[0060] 5) The sample was freeze-dried to obtain cellulose nanocrystalline solid (a-CNC) afte...

Embodiment 3

[0067] 1. Preparation of aldehyde-modified cellulose nanocrystal solution:

[0068] 1) Weigh the same mass of cellulose nanocrystals (CNC) and NaIO 4 .

[0069] 2) Take enough deionized water to dissolve CNC and NaIO 4 , adding dilute sulfuric acid (concentration 0.1mol / L) to adjust pH=3.

[0070] 3) In a water bath at a constant temperature of 40°C and protected from light while stirring, react for 3 hours.

[0071] 4) After the reaction is completed, the sample is centrifuged with deionized water, and then dialyzed for three days with a 4000 mesh dialysis bag (changing deionized water every 2h), until no IO 3 — Residue: first drop a small amount of centrifuged supernatant on the starch potassium iodide test paper, then add a small amount of dilute sulfuric acid dropwise until the test paper does not change color; titrate the dialyzed sample, and the aldehyde content is 16.27%.

[0072] 5) The sample was freeze-dried to obtain cellulose nanocrystalline solid (a-CNC) afte...

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Abstract

The invention discloses a viscoelastic hydrogel as well as a preparation method and application thereof. The viscoelastic hydrogel is prepared by compounding aldehyde-modified cellulose nanocrystalline and collagen. The viscoelastic hydrogel has good viscoelasticity and high mechanical strength, is capable of well protecting live cells in the gel, can be used as a cell support medium, meanwhile, has a good property of shearing dilution, has good syringeability, can be used for preparing cell injection medium materials, 3D printing materials for cell supporting, medicine carrier materials, cellprotection materials, and the like, and meanwhile, is simple in preparation method, cheap in raw material, easy in raw material obtaining, convenient and rapid in actual operation, not harsh in preservation condition and applicable to application and large-scale production.

Description

technical field [0001] The invention relates to the field of injectable hydrogels, in particular to a viscoelastic hydrogel and its preparation method and application. Background technique [0002] Articular cartilage defect is a common clinical disease. Because cartilage tissue is in an environment without blood vessels, innervation, and lymphatic drainage, its self-repair ability is limited. Once cartilage is damaged, it will be difficult to regenerate. [0003] Traditional treatment methods include autologous cell transplantation, microfracture, joint replacement, etc., among which the method of autologous cell transplantation is limited due to the limited source of cells, and it is easy to cause damage or dysfunction of the sampling site, so its application is greatly limited; while microfracture The cartilage induced by the scheme is fibrous. Our normal cartilage tissue is composed of hyaline cartilage. The mechanical properties of fibrous cartilage are poor and cannot ...

Claims

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Application Information

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IPC IPC(8): C08L1/04C08L89/00C08J3/075A61L27/20A61L27/24A61L27/52
CPCA61L27/20A61L27/24A61L27/52A61L2400/06A61L2430/02C08J3/075C08J2301/04C08J2389/00C08J2401/04C08J2489/00
Inventor 肖芸张爽黄丹阳林海张兴栋
Owner SICHUAN UNIV
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