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Method for extracting fatty acids from Dioscorea opposita Thumb

An extraction method and a fatty acid technology, which are applied in the field of extraction of fatty acids from Chinese yam, can solve problems such as insufficient availability of fatty acid components in Huai yam, and achieve the effects of short extraction period, simple method, and prevention of browning.

Pending Publication Date: 2020-02-11
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing research on Chinese yam focuses on functional substances such as polysaccharides and flavonoids, and the utilization of fatty acid components in Chinese yam is not enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Raw material processing: choose 100g of fresh yam with round shape, smooth surface and no pests and diseases, rinse with clean water and set aside;

[0025] Peeling: put 1% NaOH in a pot, heat up to 60-80°C, put Chinese yam into blanching treatment, take it out after 3-5 minutes, rinse with water and peel;

[0026] Color protection: Cut the peeled Chinese yam into 2 mm slices, then spray 0.1% phytic acid, and use microwave to protect the color to prevent the Chinese yam from browning. The microwave frequency is 60w, and the time is 30-60s;

[0027] Pulping: Process the processed Huayam tablets with a high-speed tissue masher (speed 12000 r / min) for 30 s to obtain Huayam pulp;

[0028] Centrifugation: Centrifuge the above-mentioned yam pulp at 10000-120000r / min for 3min, and separate the precipitate and mucus supernatant;

[0029] Enzymolysis: Take 0.5g of compound enzyme, including 50 parts of cellulase, 10 parts of mannanase, and 10 parts of xylanase, and put it in a ...

Embodiment 2

[0034] Raw material processing: choose 100g of fresh yam with round shape, smooth surface and no pests and diseases, rinse with clean water and set aside;

[0035] Peeling: put 3% NaOH in a pot, heat up to 60-80°C, put Chinese yam into blanching treatment, take it out after 3-5 minutes, rinse with water and peel;

[0036] Color protection: Cut the peeled Chinese yam into 5 mm slices, then spray 0.3% phytic acid, and use microwave to protect the color to prevent the Chinese yam from browning. The microwave frequency is 700w, and the time is 30-60s;

[0037] Pulping: Process the processed Huayam tablets with a high-speed tissue masher (speed 12000 r / min) for 30 s to obtain Huayam pulp;

[0038] Centrifugation: Centrifuge the above-mentioned yam pulp at 10000-120000r / min for 5min, and separate the precipitate and mucus supernatant;

[0039] Enzymolysis: take 0.3g of compound enzyme, including 60 parts of cellulase, 20 parts of mannanase, and 20 parts of xylanase, and put it in a...

Embodiment 3

[0044] Raw material processing: choose 100g of fresh yam with round shape, smooth surface and no pests and diseases, rinse with clean water and set aside;

[0045] Peeling: put 2% NaOH in a pot, heat up to 60-80°C, put Chinese yam into blanching treatment, take it out after 3-5 minutes, rinse with water and peel;

[0046] Color protection: Cut the peeled Chinese yam into 2mm slices, then spray 0.2% phytic acid, and use microwave to protect the color to prevent the Chinese yam from browning. The microwave frequency is 500w, and the time is 30-60s;

[0047] Pulping: Process the processed Huayam tablets with a high-speed tissue masher (speed 12000 r / min) for 30 s to obtain Huayam pulp;

[0048] Centrifugation: Centrifuge the above-mentioned yam pulp at 10000-120000r / min for 4min, and separate the precipitate and mucus supernatant;

[0049] Enzymolysis: Take 0.3g of compound enzyme, including 55 parts of cellulase, 15 parts of mannanase, and 15 parts of xylanase, and put it in a ...

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Abstract

The invention discloses a method for extracting fatty acids from Dioscorea opposita Thumb. The method comprises the following steps: S1, carrying out peeling with alkali liquor; S2, adding 0.1%-0.3% of phytic acid under a microwave condition for color protection; S3, performing pulping and centrifuging; S4, adding complex enzymes to reduce the viscosity of mucus, wherein the complex enzymes comprise cellulase, mannase and xylanase; and S5, performing spray drying, and carrying out supercritical extraction via carbon dioxide to obtain fatty acids from Dioscorea opposita Thumb. According to theinvention, CO2 is used as an extraction agent in a supercritical state, so solvent consumption is low, and product yield is high; a carbon dioxide fluid is a green pollution-free solvent and is cheapand easily-available; the extracted fatty acids from Dioscorea opposita Thumb have the characteristics of high purity, no solvent residue, short extraction period, no environmental pollution and the like; and the method is simple in process and suitable for industrial mass production.

Description

technical field [0001] The invention relates to the field of food, in particular to a method for extracting fatty acids from Chinese yam. Background technique [0002] In addition to the three major nutrients of starch, fat and protein, Chinese yam also contains a variety of vitamins and trace elements, with comprehensive nutritional value. Vegetable oil is the main source of essential fatty acids for the human body, and it is also an important raw material for food, spices, and chemicals. Wang Yong et al[2] used petroleum ether as a solvent to Soxhlet extract the oil in Chinese yam, and the obtained fatty acid was methylated by sulfuric acid-methanol method, and the fatty acid methylated product was separated and identified by gas chromatography-mass spectrometry. The relative content of each component was determined by peak area normalization method. Results A total of 27 kinds of fatty acids were detected, 18 kinds of saturated fatty acids accounted for 51% of the total...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/04C11B1/10
CPCC11B1/025C11B1/04C11B1/104
Inventor 段丽萍朱颖苗丽坤陈胜魏海帅
Owner HUBEI CHINA TOBACCO IND
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