Microbial-type time-temperature indicator
A technology of temperature indicators and microorganisms, applied in the direction of microorganisms, separation of microorganisms, methods based on microorganisms, etc., can solve the problems of complex research, complicated acquisition methods, and harmfulness of raw materials, and achieve mild preparation conditions, simple preparation process, and improved The effect on survival odds
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preparation example Construction
[0041] Further, the preparation method of the MRS solid medium includes the following steps: heating and dissolving the MRS agar medium in distilled water, correcting the pH to 6.5±0.2 with a mass fraction of 13-18% sodium hydroxide solution, and then subpackaging and sealing Afterwards, autoclave at 121°C±1°C for 12-18min.
[0042] Preferably, the ratio of the microbial microcapsules to the pH-adjusted matrix in step S4 is 1g:(1-3mL).
[0043] Preferably, the ratio of the MRS agar medium to distilled water is (65-70g) / 1000mL.
[0044] Further, the preparation method of the microbial microcapsules comprises the following steps:
[0045] (1) Wipe the SPG equipment with alcohol, turn on the sterilization lamp of the purification workbench, and fully sterilize the SPG equipment in the purification workbench for 1-2 hours;
[0046] (2) Put the SPG film after autoclaving into an Erlenmeyer flask equipped with a continuous phase, seal it with kraft paper, put it into an ultrasonic c...
Embodiment 1
[0061] This example provides a kind of microbial time-temperature indicator, is characterized in that, is made by following steps:
[0062] Step S1: Put the cell culture plate into the clean workbench, turn on the sterilization lamp and fully sterilize it for 1 hour;
[0063] Step S2: After mixing the pH mixed indicator and the MRS solid medium at a volume ratio of 1:9, the matrix is prepared;
[0064] Step S3: using 0.1mol / L sodium hydroxide standard solution to adjust the pH of the matrix to 7;
[0065] Step S4: Use the microbial microcapsules and the above pH-adjusted matrix, mix evenly, take the solidified mixture to sterilize, seal it and place it in a constant temperature water bath at 40°C; The solidified mixture was moved into the cell culture plate, and the solid microbial time-temperature indicator was prepared; the ratio of the microbial microcapsules to the pH-adjusted matrix was 1g:1mL.
[0066] The pH mixed indicator is prepared by mixing equal volumes of the...
Embodiment 2
[0082] This example provides a microbial time-temperature indicator, which is basically the same as that in Example 1, except that the mixed pH indicator is prepared by mixing equal volumes of the following solutions: bromocresol violet solution, bromine Cresol green solution, methyl orange solution; The concentration of the bromocresol purple solution is 0.03g / mL; The concentration of the bromocresol green solution is 0.05g / mL; The concentration of the methyl orange solution is 0.09 g / mL; The solvents of the bromocresol violet solution, bromocresol green solution and methyl orange solution are 94% ethanol by volume fraction.
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