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Modified epsilon-polylysine and preparation method thereof, and application of modified epsilon-polylysine in turbot preservation

A technology of polylysine and turbot, which is applied in the direction of chemical preservation of meat/fish, food ingredients as antimicrobial preservation, food ingredients containing organic compounds, etc., can solve unsatisfactory, short antibacterial time, freshness In the later stage, the ideal effect cannot be achieved, and the effect of prolonging the shelf life, simple preparation method, and mild reaction conditions is achieved.

Inactive Publication Date: 2020-02-21
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of its application, it was found that its antibacterial effect is short, and the post-preservation period cannot achieve the desired effect, and cannot meet the requirements of long-term storage of food. Therefore, it is urgent to modify its structure and develop a new type of ε-PL with long-term antibacterial effect. , improve its long-term antibacterial performance, and develop its various applications, providing a theoretical reference for its greater market value

Method used

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  • Modified epsilon-polylysine and preparation method thereof, and application of modified epsilon-polylysine in turbot preservation
  • Modified epsilon-polylysine and preparation method thereof, and application of modified epsilon-polylysine in turbot preservation
  • Modified epsilon-polylysine and preparation method thereof, and application of modified epsilon-polylysine in turbot preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The synthetic route of modified ε-polylysine is as follows:

[0040]

[0041] The specific synthetic steps of modified ε-polylysine are as follows:

[0042] to N α - tert-butoxycarbonyl-L-lysine (0.24g, 1mmol), tert-butylisonitrile (0.18g, 2mmol) was added to the MeOH suspension of benzaldehyde (0.23 g, 2mmol), and the resulting mixture was stirred at room temperature for 48 After 2 hours, the methanol was spun off on a rotary evaporator, and the crude product was dissolved in CH 2 Cl 2 , adding petroleum ether for multiple precipitations, filtering, and drying the obtained solid under vacuum conditions to obtain a white solid polymer 1.

[0043] To polymer one (1.0 g) anhydrous CH at 0 °C 2 Cl 2 Trifluoroacetic acid (1 mL) was slowly added to the solution, the mixture was stirred at room temperature for 3 h, and the CH 2 Cl 2 , and then add petroleum ether to precipitate several times, filter and dry to obtain a white solid which is modified ε-polylysine.

...

Embodiment 2

[0048] The specific synthetic steps of modified ε-polylysine are as follows:

[0049] to N α - tert-butoxycarbonyl-L-lysine (0.24g, 1mmol), tert-butylisonitrile (0.27g, 3mmol) was added to the MeOH suspension of benzaldehyde (0.345 g, 3mmol), and the resulting mixture was stirred at room temperature for 72 After 2 hours, the methanol was spun off on a rotary evaporator, and the crude product was dissolved in CH 2 Cl 2 , adding petroleum ether for multiple precipitations, filtering, and drying the obtained solid under vacuum to obtain a white solid polymer 1.

[0050] To polymer one (1.0 g) anhydrous CH at 0 °C 2 Cl 2 Trifluoroacetic acid (1.1 mL) was slowly added to the solution, the mixture was stirred at room temperature for 6 hours, and the CH 2 Cl 2 , and then add petroleum ether to precipitate several times, filter and dry to obtain a white solid which is modified ε-polylysine. The present embodiment modified ε-polylysine 1 H NMR spectrum as figure 1 , see the in...

Embodiment 3

[0053] The specific synthetic steps of modified ε-polylysine are as follows:

[0054] to N α - tert-butoxycarbonyl-L-lysine (0.24g, 1mmol), tert-butylisonitrile (0.36g, 4mmol) was added to the MeOH suspension of benzaldehyde (0.46 g, 4mmol), and the resulting mixture was stirred at room temperature for 96 After 2 hours, the methanol was spun off on a rotary evaporator, and the crude product was dissolved in CH 2 Cl 2 , adding petroleum ether for multiple precipitations, filtering, and drying the obtained solid under vacuum to obtain a white solid polymer 1.

[0055] To polymer one (1.0 g) anhydrous CH at 0 °C 2 Cl 2 Trifluoroacetic acid (1.2 mL) was slowly added to the solution, the mixture was stirred at room temperature for 8 hours, and the CH 2 Cl 2 , and then add petroleum ether to precipitate several times, filter and dry to obtain a white solid which is modified ε-polylysine. The present embodiment modified ε-polylysine 1 H NMR spectrum as figure 1 , see the inf...

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Abstract

The invention provides modified epsilon-polylysine and a preparation method thereof, and application of the modified epsilon-polylysine in turbot preservation, belonging to the field of food preservation. The invention particularly provides the preparation method and a structural formula of the modified epsilon-polylysine, and the application of the modified epsilon-polylysine in turbot preservation. The preparation method has the beneficial effects of simple process and easy experimental operation. The antibacterial activity and fresh-keeping performance of the modified epsilon-polylysine areobviously superior to the antibacterial activity and fresh-keeping performance of epsilon-polylysine; the antibacterial aging of the modified epsilon-polylysine is obviously superior to the antibacterial aging of epsilon-polylysine; and the modified epsilon-polylysine can effectively prolong the shelf life of turbots.

Description

technical field [0001] The invention relates to a modified ε-polylysine, a preparation method and its application in the preservation of turbot, belonging to the field of preservation of aquatic products. Background technique [0002] ε-polylysine (ε-PL) is a homopolymer formed by the essential amino acid lysine through the dehydration condensation of ε-amino group and α-carboxyl group to form an amide bond, forming a polymerization degree of 25~35 Amino acid linear polymer is a homotype monomer polypeptide polymer. ε-PL is purified from the culture broth of Streptomyces chromogenes controlled fermentation of amylase by means of ion exchange resin, and is a natural product with antiseptic function. ε-PL has good water solubility and thermal stability, and its aqueous solution will not decompose after autoclaving at 120°C for 20 minutes. ε-PL and its hydrochloride will be decomposed into lysine (one of the eight essential amino acids) after entering the human digestive trac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/48C08G69/10A23B4/20
CPCA23B4/20A23V2002/00C08G69/10C08G69/48A23V2200/10A23V2250/30
Inventor 李秋莹孙彤励建荣钟克利朱鸿伟李颖畅李婷婷李学鹏谢晶牟伟丽郭晓华
Owner BOHAI UNIV
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