A detection method for fluorescence-enhanced glutathione
A glutathione, fluorescence enhancement technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., to achieve the effects of low cost, good water solubility, and good biocompatibility
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Embodiment 1
[0033] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing 100 μL 50mmol / L copper nitrate solution and 1mL 150mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution, mixing with 0.1 The pH of the mol / L NaOH solution was adjusted to 5.0, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 500 W for 10 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescence emission peak of the red fluorescent copper nanocluster is around 618nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescence with a quantum yield of 0.04%.
Embodiment 2
[0035] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing and shaking 100 μL 70 mmol / L copper nitrate solution and 500 μL 120 mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution with 0.1 The pH of the mol / L NaOH solution was adjusted to 5.7, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 150 W for 20 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescent emission peak of the red fluorescent copper nanocluster is around 630nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescent light with a quantum yield of 0.09%.
Embodiment 3
[0037] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing and shaking 100 μL 100 mmol / L copper nitrate solution and 300 μL 100 mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution with 0.1 The pH of the mol / L NaOH solution was adjusted to 6.3, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 300 W for 15 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescence emission peak of the red fluorescent copper nanocluster is around 642nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescence with a quantum yield of 0.31%.
[0038] Add 100 μL of red fluorescent copper nanocluster aqueous solution and 1 mL of phosphate buffer solution (PBS, pH=6.3 0.03 mol / L) into the UV cuvette and fluorescence cuvette, and measure the UV-visible absorption spectrum and fluorescence excitation and Emiss...
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