A detection method for fluorescence-enhanced glutathione

Abstract

The invention discloses a detection method of fluorescence-enhanced glutathione. The invention uses an ultrasonic method to use 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt as a protective agent and a reducing agent, and copper nitrate solution as a base. A red fluorescent copper nanocluster aqueous solution was prepared. In the synthesis process, the addition of common reducing agents such as hydrazine hydrate and ascorbic acid is avoided, and the operation is simplified and environmentally friendly. The prepared red fluorescent copper nanocluster has a fluorescence quantum yield of 0.31%, has the advantages of large Stocks shift, good water solubility, and strong anti-photobleaching ability, and has a high sensitivity and high selectivity response to glutathione. Applied to the detection of fluorescence-enhanced glutathione.

Description

technical field

[0001] The invention relates to the preparation of copper nano-clusters, in particular to a method for detecting fluorescence-enhanced glutathione. Background technique

[0002] Metal nanoclusters are ultra-small nanoparticles with a size of less than 2nm, which have the advantages of strong fluorescence, large Stokes shift, good photostability and water solubility, and strong resistance to photobleaching. In the field of physics and chemistry, as an advanced material with catalytic properties, it can also be applied to many necessary fields such as biology, medicine, material science and national defense. However, since the particle size of metal nanoclusters is generally less than 2nm, the surface energy is very large, and they are easy to agglomerate into large particles that do not emit light. Therefore, the preparation of metal nanoclusters with good stability has very important scientific significance. Compared with nanoclusters of noble metals such as...

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