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A detection method for fluorescence-enhanced glutathione

A glutathione, fluorescence enhancement technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., to achieve the effects of low cost, good water solubility, and good biocompatibility

Active Publication Date: 2021-07-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, fluorescent probes that can specifically detect glutathione in complex living organisms without interference from other thiols still have great challenges.

Method used

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  • A detection method for fluorescence-enhanced glutathione
  • A detection method for fluorescence-enhanced glutathione
  • A detection method for fluorescence-enhanced glutathione

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing 100 μL 50mmol / L copper nitrate solution and 1mL 150mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution, mixing with 0.1 The pH of the mol / L NaOH solution was adjusted to 5.0, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 500 W for 10 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescence emission peak of the red fluorescent copper nanocluster is around 618nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescence with a quantum yield of 0.04%.

Embodiment 2

[0035] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing and shaking 100 μL 70 mmol / L copper nitrate solution and 500 μL 120 mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution with 0.1 The pH of the mol / L NaOH solution was adjusted to 5.7, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 150 W for 20 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescent emission peak of the red fluorescent copper nanocluster is around 630nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescent light with a quantum yield of 0.09%.

Embodiment 3

[0037] A preparation method of red fluorescent copper nanoclusters, comprising the following steps: mixing and shaking 100 μL 100 mmol / L copper nitrate solution and 300 μL 100 mmol / L 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt solution with 0.1 The pH of the mol / L NaOH solution was adjusted to 6.3, stirred at room temperature for 10 min, and then the reaction mixture was sonicated at a power of 300 W for 15 min to obtain an aqueous solution of red fluorescent copper nanoclusters. The fluorescence emission peak of the red fluorescent copper nanocluster is around 642nm, and when observed with a black background under an ultraviolet lamp, it presents red fluorescence with a quantum yield of 0.31%.

[0038] Add 100 μL of red fluorescent copper nanocluster aqueous solution and 1 mL of phosphate buffer solution (PBS, pH=6.3 0.03 mol / L) into the UV cuvette and fluorescence cuvette, and measure the UV-visible absorption spectrum and fluorescence excitation and Emiss...

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Abstract

The invention discloses a detection method of fluorescence-enhanced glutathione. The invention uses an ultrasonic method to use 2-mercapto-5-benzimidazole sulfonic acid dihydrate sodium salt as a protective agent and a reducing agent, and copper nitrate solution as a base. A red fluorescent copper nanocluster aqueous solution was prepared. In the synthesis process, the addition of common reducing agents such as hydrazine hydrate and ascorbic acid is avoided, and the operation is simplified and environmentally friendly. The prepared red fluorescent copper nanocluster has a fluorescence quantum yield of 0.31%, has the advantages of large Stocks shift, good water solubility, and strong anti-photobleaching ability, and has a high sensitivity and high selectivity response to glutathione. Applied to the detection of fluorescence-enhanced glutathione.

Description

technical field [0001] The invention relates to the preparation of copper nano-clusters, in particular to a method for detecting fluorescence-enhanced glutathione. Background technique [0002] Metal nanoclusters are ultra-small nanoparticles with a size of less than 2nm, which have the advantages of strong fluorescence, large Stokes shift, good photostability and water solubility, and strong resistance to photobleaching. In the field of physics and chemistry, as an advanced material with catalytic properties, it can also be applied to many necessary fields such as biology, medicine, material science and national defense. However, since the particle size of metal nanoclusters is generally less than 2nm, the surface energy is very large, and they are easy to agglomerate into large particles that do not emit light. Therefore, the preparation of metal nanoclusters with good stability has very important scientific significance. Compared with nanoclusters of noble metals such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/188G01N21/6428
Inventor 张彦张雨婷高鹏飞张国梅李天栋董川双少敏
Owner SHANXI UNIV
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