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A strain of Mortierella alpina 0901 and its application and fungal agent
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A technology of Mortierella alpina and fungal agent, applied in the direction of application, biocide, fungi, etc., to achieve the effect of ensuring high quality and safety, simple production process and low cost
Active Publication Date: 2021-05-07
HENAN AGRICULTURAL UNIVERSITY
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However, there is no report about the role of Mortierella alpina in agriculture.
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[0040] The present invention also provides a bacterial agent, the preparation method of which comprises: inoculating the Mortierella alpina 0901 described in the above technical scheme on a PDA solid medium and culturing it to obtain a Mortierella alpina mycelial block; The Mortierella alpina mycelia block was inoculated in the PDA liquid medium for shaking culture for 3 to 5 days to obtain a shaking culture; the shaking culture was inoculated in a solid material for cultivation, when the mycelium of Mortierella alpina was covered with the solid material After that, the bacterial agent is obtained.
[0041] The present invention has no special limitation on the inoculum amount and the inoculation method of inoculating the Mortierella alpina 0901 on the PDA solid medium, it can be done conventionally, and there is no special limitation on the conditions of the cultivation as well. The conditions for culturing on PDA solid medium are sufficient.
[0042] The present invention p...
Embodiment 1
[0047] Implementation steps:
[0048] Step 1: Inoculate the well-growing species of Mortierella alpina 0901 (preservation number: CGMCC No. 17791) into PDA liquid medium, and culture it on a shaker at 28° C. and 180 rpm for 5 days.
[0049] Step 2: filter the Mortierella alpina cultured in liquid under sterile conditions, and rinse the filtered mycelia with sterile deionized water.
[0050] Step 3: Dissolve 20g of clean mycelium in 100mL of sterile deionized water, add the bacterial solution to 10kg of soil, mix well and put in pots.
[0051] Step 4: Plant the crops after adequate watering.
Embodiment 2
[0061] Implementation steps:
[0062] Step 1: inoculate the well-grown Mortierella alpina species into PDA liquid medium, and cultivate in the dark at 28° C. and 180 rpm on a shaker for 5 days.
[0063] Step 2: Inoculate the fermented mixture into the sterilized brancorn flour stalk bean cake powderfulvic acid medium (5 parts of wheat bran, 5 parts of corn flour, 60 parts of corn stalks, 25 parts of bean cake powder, 5 parts of fulvic acid and water 100 parts), 28 ℃ static culture, until the mycelia completely cover the culture medium.
[0064] Step 3: Take out the mycelia in different Erlenmeyer flasks and mix them well to obtain the bacterial agent.
[0065] Step 4: Accurately weigh 20g of the bacterial agent, add it to 7.5kg of soil, mix well and put it in a pot.
[0066] Step 5: Plant the crops after adequate watering.
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technical field [0001] The invention belongs to the technical field of microbial inoculum, and in particular relates to Mortierella alpina 0901 and its application and inoculum. Background technique [0002] Some soil fungi promote crop growth by stimulating the secretion of crop growth hormones, promoting the transformation of soil nutrients, and improving the uptake of nutrients by crops. Zygomycetes are a group of saprophytic fungi with high diversity and abundance. Among the Zygomycota, Mortierella fungi have the ability to decompose a variety of recalcitrant organic compounds, can use the organic matter that is difficult to use in the soil for growth and reproduction, and at the same time promote the transformation of soil nutrients. It can also resist the growth and reproduction of certain fungi and bacterial pathogens by synthesizing a variety of antibiotics. [0003] Yamada et al first reported a strain of Mortierella elongata (Mortierella elongata 1S-5) in 1987, a...
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