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Bacillus licheniformis-containing composite microbial inoculum for degrading straw and application thereof

The technology of Bacillus licheniformis and compound bacterial agent is applied in the field of compound bacteria, which can solve the problems of unreasonable development of straw resources, limited utilization of microbial degradation straw, and poor straw degradation effect, so as to reduce the incidence of diseases and insect pests and inhibit growth. Effects of reproduction, dark green leaves

Active Publication Date: 2020-03-03
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the temperature in the north is very low for a long time, the straw is not easy to decompose in a short period of time, so the straw resources have not been rationally developed for a long time. Except for a small amount used for gaskets, feeding livestock, and some used for composting, most of them are burned as living fuel. However, the cellulose content in the straw is high, and there is also a small amount of lignin, which is very stable at room temperature, so the degradation is very slow
There are many domestic straw decomposing agents, but most of them have poor degradation effect on straw due to the limitation of ambient temperature or bacterial growth rate, and the utilization of microbial degradation of straw is even more limited.

Method used

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  • Bacillus licheniformis-containing composite microbial inoculum for degrading straw and application thereof
  • Bacillus licheniformis-containing composite microbial inoculum for degrading straw and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The mutagenic screening of embodiment 1 verrucous Myromyces

[0020] (1) Morphological identification and screening

[0021] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0022] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0023] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0029] The low-temperature acclimatization of embodiment 2 bacterial strains

[0030] (1) Enrichment culture strains

[0031] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L

[0032] Natural corn stalk powder shaker culture: Weigh 5 g of the strain source sample and add it to 100 mL corn stalk powder medium with natural corn stalk powder as the only carbon source (replacing the filter paper in the PDA medium with corn stalk powder, culture at 28°C and 150 rpm On ...

Embodiment 3

[0045] The preparation of embodiment 3 composite bacterial agents

[0046] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacillus licheniformis and Bacillus licheniformis were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial liquid reached 2.0×10 8 individual / mL.

[0047] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 10% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% of Bacillus plasmodia, 10% of Manganese peroxidase-producing Leysia lanceolata, and 30% of Bacillus licheniformis, and fully stirred and mixed to obtain a mixed bacterial liquid.

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Abstract

The invention provides a Bacillus licheniformis-containing composite microbial inoculum for effectively degrading straw at low temperature. The compound microbial inoculum is composed of six strains,and can increase the maturity days of corn straw by 2 to 3 days in an environment with the outdoor temperature being 1 to 6 DEG C, and is especially suitable for northern regions. Mixing and composting the composite microbial inoculum of the invention with corn stalk and returning the mixture to the field has a better effect on planting cucumbers. Compared with a control group, the invention has the following advantages: the plant grows more vigorously and robustly, leaves are greener, fruits are bigger, the average weight of a single fruit increases by 21g, the yield increases by 613kg per mu, and the incidence of diseases and insect pests decreases by 4.1%. The composite microbial inoculum provided by the invention and a large amount of Bacillus licheniformis can produce anti-active substances when decomposed corn stalks are returned to the field to plant cucumbers, and the composite microbial inoculum has a unique microbial oxygen consumption mechanism, which can inhibit the growthand reproduction of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a composite bacterium containing bacillus licheniformis that can effectively degrade straw at low temperature and its application. Background technique [0002] Straw is rich in lignocellulose, which is the renewable resource with the largest reserves on the earth. At the same time, its degradation and transformation by microorganisms are also an important part of the natural carbon cycle. If these resources can be fully utilized, it will be of great help to solve the energy shortage that the world is facing today. Issues such as environmental pollution have far-reaching implications. At present, many researchers have carried out research on adding exogenous lignocellulose-degrading bacteria to the composting process of agricultural waste and municipal waste. There is still controversy, but the role of adding exogenous lignocellulosic degrading bacteria to compost has been the focu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/00C05F11/08C05F17/00C12R1/07C12R1/01C12R1/645C12R1/10
CPCC12N1/20C12N1/14C05F11/00C05F11/08C05F17/00Y02W30/40
Inventor 孙旸陈光张斯童孙春玉高祎妍苏瑛杰
Owner JILIN AGRICULTURAL UNIV
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