A preparation method and application of cytochalasin compounds
A technology of cytochalasin and compounds, which is applied in the field of preparation of cytochalasin compounds, can solve the problems of low content, low yield, and affecting the development and application of compounds, and achieve the effect of high content and simple preparation method
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Embodiment 1
[0026] (1) Preparation of seed culture medium: the strain of Ceruleus cerevisiae GDGJ-368 was stored in PDB-glycerol medium at -80°C. Get Xylaria sp. GDGJ-368 (Xylaria sp.) bacterial classification and inoculate on the PDA plate culture medium, cultivate in 28 ℃ incubator for 4 days, obtain seed culture medium; PDA plate culture medium is mainly made of the following composition: Potato 200g / L, glucose 20g / L, agar powder 15g / L.
[0027] (2) Fermentation culture: the seed medium obtained in step (1) is cut into broad bean-sized thallus with an inoculation loop, inoculated into sterilized rice medium for fermentation, and the components contained in the rice medium Including: 50g of rice, 120mL of distilled water, using a conical flask with a capacity of 1000mL, inoculating a piece of broad bean-sized bacteria in each rice culture medium, standing it for 25 days at room temperature, and fermenting to obtain a solid fermented product. Then the fermented product was soaked and e...
Embodiment 2
[0033] (1) Preparation of seed culture medium: the strain of Ceruleus cerevisiae GDGJ-368 was stored in PDB-glycerol medium at -80°C. Take Xylaria sp. GDGJ-368 (Xylaria sp.) strains and inoculate them on the PDA plate medium, and cultivate them in a 28°C incubator for 4-6 days to obtain the seed medium; the PDA plate medium is mainly made of the following components: Potatoes 200g / L, glucose 20g / L, agar powder 20g / L.
[0034] (2) Fermentation culture: the seed medium obtained in step (1) is cut into broad bean-sized thallus with an inoculation loop, inoculated into sterilized rice medium for fermentation, and the components contained in the rice medium Including: 90g of rice, 150mL of distilled water, using a conical flask with a capacity of 1000mL, inoculating a piece of broad bean-sized bacteria in each rice culture medium, standing it for 50 days at room temperature, and fermenting to obtain a solid fermented product. Then the fermented product was soaked and extracted 5 t...
Embodiment 3
[0044] Embodiment 3. antibacterial effect test
[0045] 1. Preparation of bacterial suspension
[0046] In the aseptic operation bench, pick a little of the four standard strains of Escherichia coli, Bacillus subtilis, beta-hemolytic streptococcus and Bacillus megaterium, inoculate them in beef extract peptone medium, and place them in a constant temperature water bath shaker at 37°C Shake culture for 18h. The cultures of each strain cultured for 18 hours were taken, and the bacterial suspension was diluted 1000 times with beef extract peptone medium for the experiment.
[0047] 2. Preparation of test solution
[0048] Cytochalasin C and cytochalasin D obtained in the present invention were freeze-dried, and each extract was dissolved in dimethyl sulfoxide to prepare 1 mg·mL -1 The initial concentration was filtered with a 0.22 μm (nylon 66) microporous membrane in a clean bench for subsequent use.
[0049] 3. Double dilution method
[0050] For each test sample, take 8 s...
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