A strain producing polysaccharides of Ophiopogon japonicus from Hubei and its application
A technology of Ophiopogon japonicus polysaccharides and strains, applied in fungi, microorganism-based methods, microorganisms and other directions, can solve the problems of small planting area, high polysaccharide extraction cost, long production cycle of tuberous roots, etc., and achieve the effects of less pollution and environmental protection in production.
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Embodiment 1
[0053] The isolation of embodiment 1 bacterial strain
[0054] (1) The samples were taken from Hubei Maidong produced at the Hubei Maidong GAP Base in Oumiao Town, Xiangyang City, Hubei Province. After collecting the tubers of Ophiopogon japonicus Hubei, use a large amount of water to wash the soil attached to the surface of the tubers of Ophiopogon japonicus Hubei, then put the tubers into a clean beaker, add deionized water, and wash them repeatedly in an ultrasonic cleaner until the water after cleaning become extremely clear;
[0055] (2) Dip the water on the surface of Ophiopogon japonicus root tuber with sterile filter paper, and then process it according to the following steps: first soak the tuber tuber with 75% (V / V) ethanol for 2 minutes, rinse it with sterile water for 3 times; Soak the tubers in sodium hypochlorite solution with an available chlorine content of 4-6% for 2 minutes, rinse them with a large amount of sterile water for 4 times, and then dip them dry w...
Embodiment 2
[0058] Identification of embodiment 2 bacterial strains
[0059] (1) The bacterial strain isolated in Example 1 was cultured on potato dextrose agar medium (PDA) at 28° C. to form colonies, and the characteristics such as colony morphology and color were observed. The result is as figure 1 As shown, the colony is brownish green in the middle and white at the edge.
[0060] (2) Perform genome sequencing on the strain isolated in Example 1, and its 18S rRNA gene sequence is shown in SEQ ID NO.1.
[0061] The sequencing results were compared on the NCBI website (http: / / blast.ncbi.nlm.nih.gov / Blast.cgi), and it was found that its sequence had the highest homology with the sequence of Talaromyces funiculosus MH590622.1, with a similarity of 99%. %. Illustrate that the bacterial strain isolated in Example 1 is a fungus of the genus Rosacea.
Embodiment 3
[0062] The fermentation of embodiment 3 bacterial strains
[0063] (1) Get the Rope-shaped Tasmania EF048 strain that is isolated from Example 1. Under sterile conditions, use an inoculation needle to pick a small amount of mycelium, insert it into a sterilized PDA medium test tube, and activate it at 28°C for 72 hours;
[0064] (2) Under sterile conditions, inoculate the activated cultured bacterial strains into sterilized potato dextrose liquid medium (PDB), shake and culture at 28° C. and 180 rpm for 72 hours to obtain seed liquid;
[0065] (3) 200g of potatoes, 20g of glucose, and 1000mL of distilled water are prepared into a liquid fermentation medium, and 100mL of the liquid fermentation medium is put into a 250mL Erlenmeyer flask, sterilized, and cooled for later use; under sterile conditions, inoculate according to 10% amount, seed liquid was inoculated in potato liquid medium, cultured at 28°C and 180rpm for 7 days to obtain a fermented solid-liquid mixture;
[0066...
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