Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhodotorula, culture method thereof and application of rhodotorula in production of biolipid

A cultivation method and bio-oil technology, applied in the field of red yeast, can solve problems such as unfavorable industrial application of red yeast, increased product impurity content, sensory and quality decline, etc., to reduce post-processing difficulty, improve bio-oil quality, and low cost Effect

Active Publication Date: 2020-03-17
NANJING UNIV OF SCI & TECH
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the oil extraction process, the pigment will inevitably dissolve in the extracted product, resulting in an increase in the impurity content of the product and a decrease in sensory and quality
This defect makes the post-oil extraction process more complex and costly, which is not conducive to the industrial application of red yeast
In addition, the optimum temperature for fermentation of Rhodotorula strains is usually 28-33°C, and a large amount of cooling water will be consumed during the fermentation process, resulting in waste of water resources and increased costs, especially in summer where normal production cannot be carried out in some areas with high temperatures

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodotorula, culture method thereof and application of rhodotorula in production of biolipid
  • Rhodotorula, culture method thereof and application of rhodotorula in production of biolipid
  • Rhodotorula, culture method thereof and application of rhodotorula in production of biolipid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The inventor isolated and screened the initial strain from a potato stem. Pick a single colony growing on the yeast extract-peptone-glucose-agar (YPD) solid medium, dilute it with normal saline and spread it on fresh YPD medium. After a single colony grows, re-dilute and spread it according to the above method . After 10 repetitions, the colonies were consistent with the cell shape and stable in character. The composition of the yeast extract-peptone-glucose-agar (YPD) solid medium is: 1% yeast extract, 2% peptone, 2% glucose, and 2% agar powder.

[0033] After the strain was cultured on YPD solid medium at 30°C for 3 days, the colony was nearly round, with a smooth and shiny surface, slightly raised in the middle, neat edges, and off-white ( figure 1 ). After the bacterium colony is dispersed, it is observed under a scanning electron microscope, the thalline is oval, and multiple thallines are connected into long chains ( figure 2 ). Suspend the colonies in norma...

Embodiment 2

[0036] A single colony of Rhodotorula U13N3 grown on YPD solid medium was picked and inoculated into 20 mL of a 100 mL shake flask containing seed medium, cultured at 25°C for 48 h, and the shake flask rotated at 100 rpm to obtain a seed culture solution. The composition of the seed medium is: glucose 30g / L, K 2 HPO 4 2g / L, yeast powder 1.5g / L, MgSO 4 ·7H 2O 1.5g / L, (NH 4 ) 2 SO 4 1.2g / L, pH 4.5.

[0037] Take 20mL of seed solution and inoculate into a 500mL shake flask containing 80mL of fermentation medium, the inoculation amount is 10%, and culture at 100rpm and 25°C for 6 days to obtain a fermentation medium. The composition of the fermentation medium is: glycerol 30g / L, K 2 HPO 4 2g / L, yeast powder 1.5g / L, MgSO 4 ·7H 2 O 1.5g / L, (NH 4 ) 2 SO 4 1.2g / L, pH 6.5.

[0038] The obtained fermented liquid is centrifuged to obtain the red yeast cell. Then add 6 mol / L hydrochloric acid until the final concentration of hydrochloric acid is 4 mol / L, then treat in a wat...

Embodiment 3

[0042] According to the method of Example 2, Rhodotorula U13N3 was inoculated into the seed medium, cultured at 35° C. for 24 hours, and the rotation speed of the shake flask was 300 rpm, to obtain a seed culture solution. The composition of the seed medium is: glycerol 30g / L, K 2 HPO 4 2g / L, yeast powder 1.5g / L, MgSO 4 ·7H 2 O 1.5g / L, (NH 4 ) 2 SO 4 1.2g / L, pH 5.9.

[0043] The obtained seed culture solution was inoculated into the fermentation medium with an inoculation amount of 5%, and cultured at 300 rpm and 37° C. for 9 days to obtain a fermentation medium. The composition of the fermentation medium is: glycerol 60g / L, K 2 HPO 4 2g / L, yeast powder 1.5g / L, MgSO 4 ·7H 2 O 1.5g / L, (NH 4 ) 2 SO 4 1.2g / L, pH 5.6. The obtained fermentation culture liquid is subjected to solid-liquid separation to obtain Rhodotorula thallus.

[0044] Wash the Rhodotorula thallus once with physiological saline, and extract oil according to the method described in Example 2, and fi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses rhodotorula, a culture method thereof and an application of the rhodotorula in production of biolipid. The rhodotorula U13N3 disclosed by the invention is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2019503. The rhodotorula U13N3 is characterized in that the carotene production capacity is lost, and bacterial colonies are off-white on a yeast extract-peptone-glucose-agar culture medium. The rhodotorula U13N3 can be fermented by utilizing various carbon sources, and when glycerol is used as a carbon source to be cultured at 37 DEG C, the intracellular grease content can reach 65% of the dry weight of thalli. The rhodotorula U13N3 is wide in environmental adaptability, can utilize various cheap carbon sources to produce biolipid at high yield, does not contain pigments in cells, is beneficial to reducing the impurity content during grease extraction and improving the grease quality, and has a good application prospect.

Description

technical field [0001] The invention relates to a red yeast strain, its cultivation method and its application in the production of bio-oil, belonging to the technical field of microbial fermentation. Background technique [0002] Biodiesel is a renewable fuel made from bio-oil as raw material through simple chemical processing. It has a similar calorific value to petrochemical diesel, but its pollutant emissions are lower than the latter. It is one of the more ideal biofuels. The raw materials for the current production of biodiesel mainly come from vegetable oil and some animal fats. The supply of oil raw materials is greatly affected by climate and environment, and the quantity and quality fluctuate, which is not conducive to the large-scale production of biodiesel. Oleaginous microorganisms (Oleaginous microorganisms) can convert carbohydrates, hydrocarbons and ordinary oils as carbon sources into a large amount of oil stored in the bacteria under certain conditions, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/64C12R1/645
CPCC12N1/16C12P7/6463C12R2001/645C12N1/145
Inventor 李晶张涛赵一涵张建法
Owner NANJING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com