Application of a kind of Trichoderma dark viridans hb20111 in the control of wheat stem rot and sheath blight
A technology of HB20111, Trichoderma dark green, applied in the direction of application and use of spores, botanical equipment and methods, etc., can solve environmental and food safety hazards, can not fundamentally improve the aggravation and spread of soil-borne diseases, etc. problems, to achieve the effect of reducing the use, improving the quality of agricultural products, and reducing pesticide residues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Example 1 Preparation of High Concentration Spore suspension
[0025] Deep Green Wooden Muscle HB20111 is obtained with a large amount of solid fermentation method, and the specific preparation method includes the following steps:
[0026] 1 Solid first-level seed preparation: PDA medium, inoculation of deep green wood HB20111 test tube strain, cultured in test tube medium, 24 ° C, refrigerator, requires fungi and blended spores, no cessica; Then, inoculate the cylindrical bottle, PDA medium, 25 ° C culture, 8 days of bacteria, requires no liquid water in the surface of the colony, and the bacteria and the blend of spores, no cessica pollution;
[0027] 2 Liquid seed preparation: Flush the surface of the melon bottle using 115 ° C for 30 minutes of glucose water, washed fresh spores, diluted to 5 × 10 6 Spore / ml as a liquid seed inoculated;
[0028] 3 inoculation solid medium: grain: rice husk (W / W) = 49: 5, water content is 70% (including moisture of liquid fermentatio...
Embodiment 2
[0033] Example 2 Preparation of Bio Various
[0034] Using Diatolite HB20111 spore suspension produced by Embodiment 1, a solid fungicide was formed, and 3% carboxymethylcellulose sodium and 3% alginate were mixed with stirring mixed with a mixer. Number of 2.0 × 10 8 CFU / g dried at 50 ° C in fluidized bed to less than 10% or less, and then displaced into a bag.
Embodiment 3
[0070] Using Mai 0.uca HB20111 spore suspension, a solid fungicide is formed, and 2% carboxymethylcellulose sodium and 1% alginate were mixed with a mixer, and the number of bacteria was 2.0 × 10. 8 CFU / g, dried 50 ° C in fluidized bed to 10% or less, then dispensed into a bag of products;
[0071] Among them, deep green xermewe HB20111 spore suspension is obtained with a large amount of solid fermentation method, and the specific preparation method includes the following steps:
[0072] 1 Solid first-level seed preparation: PDA medium, inoculation of deep green wood HB20111 test tube strain, cultured in test tube medium, 24 ° C, refrigerator, requires fungi and blended spores, no cessica; recovery flask was then inoculated, using PDA medium, cultured 28 deg.] C, strain age 5 days, requires no liquid surface of the colony, conidia and hypha fullness, no bacterial contamination;
[0073] 2 Liquid seed preparation: Follow the surface of the mean bottle using 115 ° C for 30 minutes...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More