Method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry

A high-performance liquid chromatography and sweetener technology, which is applied in the field of alcohol detection and analysis, can solve the problems of not meeting the detection requirements, high detection limits, time-consuming and manpower consumption, etc., to avoid matrix effects, The effect of reducing detection cost and preventing high temperature instability

Inactive Publication Date: 2020-04-03
SHAANXI XIFENG LIQUOR CO LTD
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many detection methods for sweeteners. The most commonly used methods are liquid chromatography-ultraviolet detection, liquid chromatography-fluorescence detection, ion exchange chromatography, liquid chromatography-mass spectrometry, etc. Most of the methods can only be used for single detection One or two sweeteners not only consume time and manpower, but also the precision of the detection results such as liquid chromatography-ultraviolet detection method, liquid chromatography-fluorescence detection, etc. is not enough to achieve the purpose of strict control and monitoring. Furthermore, these methods can no longer meet the testing requirements of the relevan

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry
  • Method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry
  • Method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] A high-performance liquid chromatography-mass spectrometry method for simultaneously detecting six sweeteners in Fengxiang liquor, the instrument used is high-performance liquid chromatography-mass spectrometry HPLC-MS. The method proceeds as follows:

[0036] 1.1 Materials, reagents and instruments

[0037] Sample: Finished wine, taken from Shaanxi Xifeng Wine Co., Ltd.

[0038] Reagents: formic acid, methanol, ammonium acetate (analytical grade), ultrapure water.

[0039] Instruments and equipment: high performance liquid chromatography tandem mass spectrometer (HPLC-20A high performance liquid chromatography tandem LCMS-8045 mass spectrometer from Shimadzu Corporation), micro balance (Mettler-Toledo Corporation).

[0040] Standard product: acesulfame potassium (purity≥99.4%, Dr.Ehrenstorfer company), sodium saccharin (purity≥86.2%, Dr.Ehrenstorfer company), cyclamate (purity≥99.1%, Dr.Ehrenstorfer company), sucralose ( Purity ≥ 99.7%, Dr. Ehrenstorfer Company), as...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an analysis method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry. The method comprises the steps of treating a sample in a water bath, fixing the volume, filtering with a 0.22 mu m filter membrane and putting on a machine to analyze; taking a Shin-pack XR-ODSIII C18 column as a separation column and 10% of formic acid-methanol as a mobile phase, carrying out gradient elution, carrying out high performance liquid chromatography separation, scanning in an ESI negative ion mode, and carrying out multi-reaction monitoring mode (MRM) detection. According to the method disclosed by the invention, the measurement time only needs 7 minutes, the detection efficiency is improved, the detection limit and the quantification limit are lower, and the precision of the instrument and the method is high; the method is simple, quick, accurate and reliable; a high-performance liquid phase is adopted in a liquid phase part, so that the detection cost is reduced; the pretreatment of the sample is to heat in the water bath at 60 DEG C, so that not only is the matrix effect caused by alcohol avoided, but also the situation that the aspartame is not stable at high temperature, is prevented.

Description

technical field [0001] The invention belongs to the technical field of alcohol detection and analysis, and relates to a method for detecting artificially synthesized sweeteners, in particular to a method for simultaneously detecting six kinds of sweeteners in Fengxiang liquor by high performance liquid chromatography-mass spectrometry. Background technique [0002] As a food additive, sweeteners can improve the flavor of food when added in an appropriate amount. However, long-term intake of artificial sweeteners may lead to metabolic system diseases such as hyperglycemia, glucose intolerance, obesity, irritability, Neurological abnormalities such as depression, memory loss, and Alzheimer's disease have even been reported, and artificial sweeteners such as sodium saccharin and sweeteners are genotoxic and carcinogenic. my country's "National Standards for Food Safety-Standards for the Use of Food Additives" clearly stipulates the scope of application and addition limits of ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N30/8679G01N2030/027
Inventor 阎总科刘丽丽任璐罗冠龙朱瑞雪胡瑾
Owner SHAANXI XIFENG LIQUOR CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products