Method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry

Abstract

The invention relates to an analysis method for simultaneously detecting six sweetening agents in feng-flavor liquor by high performance liquid chromatography-mass spectrometry. The method comprises the steps of treating a sample in a water bath, fixing the volume, filtering with a 0.22 mu m filter membrane and putting on a machine to analyze; taking a Shin-pack XR-ODSIII C18 column as a separation column and 10% of formic acid-methanol as a mobile phase, carrying out gradient elution, carrying out high performance liquid chromatography separation, scanning in an ESI negative ion mode, and carrying out multi-reaction monitoring mode (MRM) detection. According to the method disclosed by the invention, the measurement time only needs 7 minutes, the detection efficiency is improved, the detection limit and the quantification limit are lower, and the precision of the instrument and the method is high; the method is simple, quick, accurate and reliable; a high-performance liquid phase is adopted in a liquid phase part, so that the detection cost is reduced; the pretreatment of the sample is to heat in the water bath at 60 DEG C, so that not only is the matrix effect caused by alcohol avoided, but also the situation that the aspartame is not stable at high temperature, is prevented.

Description

technical field [0001] The invention belongs to the technical field of alcohol detection and analysis, and relates to a method for detecting artificially synthesized sweeteners, in particular to a method for simultaneously detecting six kinds of sweeteners in Fengxiang liquor by high performance liquid chromatography-mass spectrometry. Background technique [0002] As a food additive, sweeteners can improve the flavor of food when added in an appropriate amount. However, long-term intake of artificial sweeteners may lead to metabolic system diseases such as hyperglycemia, glucose intolerance, obesity, irritability, Neurological abnormalities such as depression, memory loss, and Alzheimer's disease have even been reported, and artificial sweeteners such as sodium saccharin and sweeteners are genotoxic and carcinogenic. my country's "National Standards for Food Safety-Standards for the Use of Food Additives" clearly stipulates the scope of application and addition limits of ac...

AI Technical Summary

Problems solved by technology

[0003] There are many detection methods for sweeteners. The most commonly used methods are liquid chromatography-ultraviolet detection, liquid chromatography-fluorescence detection, ion exchange chromatography, liquid chromatography-mass spectrometry, etc. Most of the methods can only be used for single detection One or two sweeteners not only consume time and manpower, but also the precision of the detection results such as liquid chromatography-ultraviolet detection method, liquid chromatography-fluorescence detection, etc. is not enough to achieve the purpose of strict control and monitoring. Furthermore, these methods can no longer meet the testing requirements of the relevant state departments for the six sweeteners.

The publication number is CN104634895A Chinese invention patent discloses a method for simultaneous detection of six sweeteners in liquor by ultra-high liquid chromatography-triple quadrupole tandem mass spectrometer. The mobile phase of the method is 0.5-2mmol ammonium acetate (0.1-0.3 % acetic acid) + methanol, the solution preparation is more complicated; the sensitivity is not high enough, the detection limit (≥10ng / mL), the limit of quantification (≥20ng / mL) is high, and the detection time is relatively long; the liquid phase part adopts ultra-high performance liquid phase , compared with HPLC, the detection cost is greatly increased

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