Breeding method of specified-pathogen-free experimental pigs and application of breeding method of specified-pathogen-free experimental pigs

Abstract

The invention provides a breeding method of specified-pathogen-free experimental pigs and application of the breeding method of the specified-pathogen-free experimental pigs, and relates to the technical field of breeding of experimental animals. The provided breeding method of the specified-pathogen-free experimental pigs comprises the following steps: making pathogen-free pregnant sows have natural deliveries in a sterile environment, transferring delivered piglets into a barrier environment, and after feeding is conducted for at most 14 days, conducting weaning to obtain the specified-pathogen-free experimental pigs, wherein feeding comprises intestinal probiotic feeding, artificial milk feeding, and solid feed ingestion induction. By means of the provided breeding method of the specified-pathogen-free experimental pigs, not only are the problems of low quality and long time of experimental pigs obtained through an ordinary method solved, but also the delivery technology and obtaining cost is controlled in a low range, and the fact that the high-quality specified-pathogen-free experimental pigs are obtained from breeding can be guaranteed at the same time.

Description

technical field [0001] The invention relates to the technical field of experimental animal breeding, in particular to a breeding method and application of a designated pathogen-free experimental pig. Background technique [0002] There are many kinds of pig diseases and various diseases are easy to interact with each other. The seemingly typical morbidity or death is often caused by mixed infection or secondary infection of various bacteria and viruses, which brings great pressure to the prevention and control of pig diseases. Antibiotics are prone to drug resistance and have no effect on viral diseases, so biological vaccines have become the only way to solve this problem. Animal experiments carried out on experimental pigs are the most important and important link in the development of pig vaccines, so the quality of experimental pigs is particularly important. [0003] The current method of obtaining experimental pigs mainly includes two aspects: one is to screen and obt...

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Problems solved by technology

[0003] The current method of obtaining experimental pigs mainly includes two aspects: one is to screen and obtain them from small scattered farmers who have the advantage of natural environment isolation. This method is low in cost, but the quality is uncontrollable. A lot of screening time, and once the candidate farms are inoculated with relevant vaccines, or when the candidate pigs are positive for the tested disease antibodies due to other means, they must find a new source of pigs; the second is to cultivate or purchase SPF experiments Pigs, although this method can obtain high-quality experimental pigs, it has the characteristics of high technical difficulty and high production costs. The acquisition process requires not only experienced caesarean section delivery personnel for sows, but also professional anesthesia, delivery and other surgical equipment

At the same time, piglets need special equipment and facilities for isolated breeding after birth, which is likely to cause greater economic pressure on researchers, and because SPF experimental pigs have extremely high requirements on the environment and breeding, they will suffer from disease and death in subsequent experiments. The risk of causing the experiment to fail is also very high

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