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A kind of colloidal particle with low protein adsorption and its preparation method and application

A technology of colloidal particles and anti-protein adsorption, which is applied in the field of biomedical materials, can solve the problems of few chemical substances, reduce protein adsorption, and high non-specific protein adsorption, and achieve simple preparation steps, large optional range, and good biocompatibility sexual effect

Active Publication Date: 2021-09-17
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even after such treatment, the non-specific adsorption of protein is still quite high, further reducing protein adsorption is still a key issue of current research
Another concern is that the aforementioned chemical modifications may introduce new biosafety risks, so not many chemicals are available

Method used

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  • A kind of colloidal particle with low protein adsorption and its preparation method and application
  • A kind of colloidal particle with low protein adsorption and its preparation method and application
  • A kind of colloidal particle with low protein adsorption and its preparation method and application

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preparation example Construction

[0055] The preparation method of the present invention will be further described in detail in conjunction with specific examples below. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0056] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents and materials used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0058] (1) Disperse silicon dioxide with a diameter of 30nm, silane coupling agent KH570, and ethyl acetate in 500ml of water for hydrolysis according to the mass fraction ratio of 1:3:5. After 12 hours, add 100mg of persulfuric acid by emulsion polymerization Potassium was polymerized at 70°C, and the obtained colloidal particles were washed several times with ethanol and water to obtain colloidal particles embedded with silicon dioxide on the surface, which was set aside.

[0059] (2) the hydrofluoric acid of 8.5g colloid particle obtained in the step (1) is mixed with 200ml5%, after stirring for 12 hours, obtain the colloid particle (product A) that the radius that has sunken structure on the surface is about 120nm, from it According to the scanning electron microscope, the diameter of the concave structure is about 25nm, and the depth is about 13nm. The colloidal particles have a hollow structure with a shell thickness of about 30 nm.

[0060] (3) Disperse 8.0 g of colloi...

Embodiment 2

[0070] (1) Disperse silica with a diameter of 12nm and silane coupling agent KH560 in 400ml of water for hydrolysis according to the mass fraction ratio of 2:3. After 12 hours, wash the obtained colloidal particles with ethanol and water several times and set aside.

[0071] (2) 7.8g of colloidal particles obtained in step (1) were mixed with 200ml of 2% hydrofluoric acid, and stirred for 24 hours to obtain solid colloidal particles (product A) with a concave structure on the surface.

[0072] (3) Disperse 7.5 g of colloidal particles obtained in step (2) in 50 ml of isopropanol, slowly add 5 g of silane coupling agent KH560, stir for 24 hours, and centrifuge to obtain colloidal particle product B.

[0073] (4) disperse the 5.8g colloidal particles obtained in step (3) in 30ml tetrahydrofuran, slowly add polyethylene glycol 8000 (molecular weight is 8000g / mol) solution with terminal modification group, after stirring for 24 hours, centrifuge The colloidal particles (product C)...

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Abstract

This article provides a colloidal particle with low protein adsorption performance and its preparation method and application; the colloidal particle has the characteristics of simple operation and preparation steps, mild reaction conditions, good anti-protein adsorption performance and good biocompatibility. Specifically: combine the anti-protein adsorption performance of the polymer chain itself with the selectivity of the surface depression size to the adsorbed protein, and through the synergistic effect of the two, further reduce the amount of protein adsorption by the material. A wide range of materials can be selected, which is conducive to the introduction of biocompatible components. The colloidal particles herein can have a cavity structure, which can be used as a drug carrier, or a carrier of other small molecule reagents, to transport the guest molecule to the targeted position, and realize the function of the material for drug delivery and drug release.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a colloidal particle with low protein adsorption and a preparation method and application thereof. Background technique [0002] In recent years, the research on biomedical materials has attracted more and more attention. This kind of biomedical material that constructs the desired function through artificial synthesis can treat and repair the functions of tissues and organs in a targeted manner in organisms. Among many biomedical materials, colloidal particles are regarded as a promising material. This is due to the fact that colloidal particles have a larger specific surface area under a certain mass, which enhances their contact area and action sites with the outside world, and maximizes their effects. The small size of colloidal particles makes them easy to enter different parts through the human circulatory system, combined with large specific surf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/32A61K47/10
CPCA61K9/5026A61K9/5031
Inventor 邱东王超
Owner INST OF CHEM CHINESE ACAD OF SCI