Room-temperature phosphorescence detection method of alkaline phosphatase and application thereof

A detection method, room temperature phosphorescence technology, applied in fluorescence/phosphorescence, biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high cost, complex detection process, large interference, etc. Good compatibility and high sensitivity

Pending Publication Date: 2020-04-10
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems of complex detection process, high cost and large interference of existing alkaline phosphatase, the present invention provides a room temperature phosphorescence detection method and application of alkaline phosphatase

Method used

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  • Room-temperature phosphorescence detection method of alkaline phosphatase and application thereof
  • Room-temperature phosphorescence detection method of alkaline phosphatase and application thereof
  • Room-temperature phosphorescence detection method of alkaline phosphatase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, room temperature phosphorescence detection of alkaline phosphatase in human serum

[0032] The first step is to prepare Mn-doped ZnS quantum dots

[0033] β-cyclodextrin, Zn(Ac) 2 and Mn(Ac) 2 Mix according to the molar ratio of 3.5:1:0.05, adjust the pH value of the system to 10.5 with NaOH, protect with nitrogen, and stir magnetically at room temperature for 40 minutes; then use a syringe to quickly add Zn(Ac) 2 Na 2 S, continue to react at room temperature for 30 min; then heat the solution to 70° C., and age in air for 1.5 h to obtain a crude product of β-CD-coated Mn:ZnS quantum dots. Sediment the quantum dots with the same volume of absolute ethanol, centrifuge at high speed, pour off the supernatant, and vacuum dry at room temperature for 24 hours to obtain the desired solid powder of quantum dots.

[0034] The second step is to prepare Mn:ZnS quantum dot mother liquor:

[0035]Weigh 50mg of Mn:ZnS quantum dots, and dilute it in a 100mL volumetric...

Embodiment 2

[0045] Example 2, room temperature phosphorescence detection of alkaline phosphatase in serum

[0046] The first step is to prepare Mn-doped ZnS quantum dots:

[0047] β-cyclodextrin, Zn(Ac) 2 and Mn(Ac) 2 Mix at a molar ratio of 3.5:1:0.03, adjust the pH of the system to 10.5 with NaOH, protect with nitrogen, and stir magnetically at room temperature for 40 minutes; then use a syringe to quickly add Zn(Ac) 2 Equimolar Na 2 S, continue to react at room temperature for 20 min; then heat the solution to 50° C., and age in air for 1.5 h to obtain a crude product of β-CD-coated Mn:ZnS quantum dots. Sediment the quantum dots with the same volume of absolute ethanol, centrifuge at high speed, pour off the supernatant, and vacuum dry at room temperature for 24 hours to obtain the desired solid powder of quantum dots.

[0048] The second step is to prepare Mn:ZnS quantum dot mother liquor:

[0049] Weigh 50mg of Mn:ZnS quantum dots, and dilute it in a 100mL volumetric flask with ...

Embodiment 3

[0059] Example 3, room temperature phosphorescence detection of alkaline phosphatase in serum

[0060] The first step is to prepare Mn:ZnS quantum dots:

[0061] β-cyclodextrin, Zn(Ac) 2 and Mn(Ac) 2 Mix at a molar ratio of 3.5:1:0.04, adjust the pH of the system to 10.5 with NaOH, protect with nitrogen, and stir magnetically at room temperature for 40 minutes; then quickly add Zn(Ac) 2 Equimolar Na 2 S, continue to react at room temperature for 20 min; then heat the solution to 50 °C, and age in air for 2 h to obtain the crude product of β-CD-coated Mn:ZnS quantum dots. Sediment the quantum dots with the same volume of absolute ethanol, centrifuge at high speed, pour off the supernatant, and dry in vacuum at room temperature for 24 h to obtain the desired solid powder of quantum dots.

[0062] The second step is to prepare Mn:ZnS quantum dot mother liquor:

[0063] Weigh 50 mg of Mn:ZnS quantum dots, and dilute it in a 100 mL volumetric flask with secondary deionized wat...

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Abstract

The invention, which belongs to the technical field of alkaline phosphatase detection, discloses a room-temperature phosphorescence detection method of alkaline phosphatase and application thereof, thereby solving problems of complex detection process, high cost and large interference of the existing alkaline phosphatase. At a room temperature, a prepared beta-cyclodextrin-modified Mn: ZnS room-temperature phosphorescent quantum dot is used as a phosphorescent probe and pyrophosphate is used as a molecular recognition unit; and analysis and detection of pyrophosphatase are realized by detecting room-temperature phosphorescence changes of a system after alkaline phosphatase is added. The response range of the phosphorescence detection system to pyrophosphatase is 0.22-10.4 U/L, and the detection limit is 0.045 U/L. The method can be used for detecting alkaline phosphatase in serum; and a complex sample pretreatment process is not needed during measurement. A deoxidant and an inducer donot need to be added for generation of room-temperature phosphorescent, and interference of background fluorescence and scattered light of an actual sample can be avoided.

Description

technical field [0001] The invention belongs to the technical field of detection of alkaline phosphatase, and in particular relates to a room temperature phosphorescence detection method and application of alkaline phosphatase. Background technique [0002] Alkaline phosphatase generally exists in almost all organisms except higher plants, can directly participate in phosphorus metabolism, and plays an important role in the digestion, absorption, secretion and ossification of calcium and phosphorus. At the same time, the detection of alkaline phosphatase is one of the routine clinical detection items, mainly used for the diagnosis of diseases of the liver and skeletal system. Because it can be used as an important biological indicator in the diagnosis of many diseases, such as primary liver cancer, secondary liver cancer, cholestatic hepatitis, osteitis fibrosa, osteomalacia and other diseases. If the ALP value in the serum is abnormal, it will lead to the occurrence of cer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/42G01N21/64
CPCC12Q1/42G01N21/64G01N2333/916
Inventor 卫艳丽秦国杰左力翔王丽董川
Owner SHANXI UNIV
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