Method for detecting comprehensive content of soil heavy metal by luminous bacteria and application thereof
A technology of luminescent bacteria and heavy metals, applied in measuring devices, chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, can solve problems that have not yet existed, and achieve fast reproduction speed, strong vitality, and great application prospects Effect
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[0024] Example 1
[0025] The method for detecting the comprehensive content of heavy metals in soil by photobacteria of this embodiment includes the following steps:
[0026] 1) Sample pretreatment:
[0027] Collect the soil near a plastic factory to remove debris, including gravel, stones, sticks, weeds, plant roots, insect corpses and rocks, and new bodies such as manganese nodules, lime nodules, etc.;
[0028] Spread the soil after removing the sundries on a drying board or wooden board lined with clean white paper and air dry naturally, and no exposure to the sun. When the soil reaches a semi-dry state, break up large pieces of soil to avoid forming hard blocks, and remove gravel, animal and plant residues at any time during the drying process. The air-drying room should be dry and ventilated, the air-drying temperature is 30-35℃, and the air-drying time is 3-7 days;
[0029] Grind the air-dried soil with a grinding rod, first pass through a 2mm nylon sieve, grind repeatedly with...
Example Embodiment
[0045] Example 2
[0046] The difference between Embodiment 2 and Embodiment 1 is:
[0047] In this example, the soil near a discontinued factory was collected as a test sample. After removing gravel, stones, weeds, and plant roots, the sample was ground. After passing through a 2mm sieve, it was ground and sieved three times until only A small amount of sand is stopped, and then passed through a 100-mesh fine sieve. The sieved soil was made into a soil suspension at the ratio of soil: water = 1:5, shaken on a shaker for 8 hours, and left for 16 hours to obtain the supernatant, filtered with a 20 micron ultrafiltration membrane, and the filtrate was rotated at a rotating speed Centrifuge at 7000rpm for 3min to obtain a colorless and transparent solution as the sample to be tested.
[0048] In this example, the relative luminous intensity of the obtained sample is 0.773%, which corresponds to the toxicity level II, and the total toxicity of the soil is equivalent to the content of h...
Example Embodiment
[0049] Example 3
[0050] The difference between Embodiment 3 and Embodiment 1 is:
[0051] In this example, the soil near the metal processing plant was collected as a test sample, and the sample was ground after removing gravel, stones, weeds, and plant roots. The sample was ground after passing through a 2mm sieve, and then sieved 5 times until there was only a small amount. The sand grains stop, and then go through a 100-mesh fine sieve. The sieved soil was made into a soil suspension at the ratio of soil: water = 1:5, shaken on a shaker for 8 hours, and left for 16 hours to obtain the supernatant, filtered with a 20 micron ultrafiltration membrane, and the filtrate was rotated at a rotating speed Centrifuge at 9000 rpm for 3 min to obtain a colorless and transparent solution as the sample to be tested.
[0052] In this example, the relative luminescence intensity of the obtained sample is 0.553%, which corresponds to the toxicity level II, and the total toxicity of the soil is...
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