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Orange peel pomace leavening agent and orange-peel-pomace-contained fermented feed for sow

A technology of starter and microbial starter, applied in the fields of biotechnology and feed processing, to achieve obvious synergies, facilitate transportation, and improve production efficiency

Pending Publication Date: 2020-04-17
漳州大北农农牧科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is a lack of effectively degrading the insoluble dietary fiber in orange peel pomace, and effectively converting it into a feed production strain of soluble dietary fiber, therefore, isolating and purifying suitable bacterial strains to improve the utilization of orange peel pomace rate, reducing the economic cost of tangerine peel feed ingredients has important social and economic significance

Method used

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  • Orange peel pomace leavening agent and orange-peel-pomace-contained fermented feed for sow
  • Orange peel pomace leavening agent and orange-peel-pomace-contained fermented feed for sow
  • Orange peel pomace leavening agent and orange-peel-pomace-contained fermented feed for sow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and preservation of Aspergillus oryzae CGMCC No.18109

[0030] 1) Bacterial enrichment culture: Dilute the collected naturally fermented soybean paste samples and inoculate them in the proliferation liquid medium, culture and proliferate at a constant temperature of 30°C for 3-5 days;

[0031] 2) Separation and purification: Dilute, smear, and culture the enrichment culture in step 1) on the proliferation agar medium, pick out a single colony with a characteristic shape, number it, and then culture it by streaking until it is a pure colony , through Gram staining microscope, to observe whether the shape of the bacteria is single, otherwise, re-stretch to separate and purify; conduct 16S rDNA detection on the suspected colonies, inoculate the colonies identified as Aspergillus oryzae on the slant of the test tube to proliferate, and then inoculate the slant The strains were stored in a refrigerator at 4°C until use;

[0032] 3) The identified Asperg...

Embodiment 2

[0033] Embodiment 2: the mensuration of aspergillus oryzae fermentation activity

[0034] Test strains: Aspergillus oryzae Huyao 3.042, donated by China Agricultural University; Aspergillus oryzae CGMCC No.3.7190, purchased from the General Microorganism Center of China Committee for Culture Collection of Microorganisms; Aspergillus oryzae CGMCC No.18109.

[0035] Test method: Determination of cellulase and amylase activity

[0036] (1) For the activation of the strains, the preserved Aspergillus oryzae Huniang 3.042, Aspergillus oryzae CGMCC3.7190 and Aspergillus oryzae CGMCC No.18109 were respectively taken, and potato dextrose agar (PDA) medium was used as the activation medium and seed medium. The formula is: 2% glucose, 2% potato, 1.5%-2.0% agar, sterilized at 115°C for 30min.

[0037] Under sterile conditions, inoculate 1 ring of Aspergillus oryzae on the PDA slant medium, culture it in a 30°C incubator for 3 days, and take it out after the slant is covered with green s...

Embodiment 3

[0043] Embodiment 3: orange peel pomace fermentation

[0044] Orange peel pomace is the waste after extracting pectin from citrus peels and lemon peels, with a moisture content of 40-60%. Citrus peel and lemon peel are considered to have the effect of invigorating the stomach and eliminating food in traditional Chinese medicine, and they can also be used in animal feed to promote food intake. At the same time, citrus peel and lemon peel are also high-quality dietary fibers, which can effectively solve the problem of sows. Constipation problem.

[0045] Test strains: Aspergillus oryzae Huniang 3.042 and Aspergillus oryzae CGMCC No.18109 with superior fermentation performance detected in Example 2 were selected as fermentation strains.

[0046] Medium: Potato Dextrose Agar (PDA) medium, formula: 2% glucose, 2% potato, 1.5%-2.0% agar, sterilized at 115°C for 30min.

[0047] experiment method:

[0048] (1) Activation: under aseptic conditions, inoculate 1 ring of Aspergillus or...

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Abstract

The invention, which belongs to the technical field of biotechnology and feed processing, particularly relates to orange-peel-pomace-contained fermented feed for sow. The invention relates to an Aspergillus oryzae strain having an accession number CGMCC No. 18109. The invention also provides a microbial leavening agent containing the strain and orange peel pomace sow feed fermented by the microbial leavening agent. After the microbial agent is used for fermenting orange peel pomace substrates, the contents of soluble dietary fibers, hesperidin and dried tangerine or orange peel polysaccharidesare all increased before fermentation. With adding of the orange peel pomace fermented feed, constipation of sows during pregnancy is obviously improved; and the number of live weaning piglets, average weaning weight and weaning survival rate are all remarkably higher than those of a control group.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and feed processing, and in particular relates to a starter of orange peel and pomace and fermented feed for sows containing orange peel and pomace. Background technique [0002] Dietary fiber has been regarded as the "seventh nutrient" by medical and nutrition experts at home and abroad, and it plays an important role: promoting gastrointestinal motility, improving intestinal development; increasing satiety; regulating intestinal microbial balance, improving intestinal health; improving Insulin sensitivity, stable blood sugar depth, lower blood cholesterol level; reduce the transfer of pathogenic bacteria from the hindgut to the anterior intestine, absorb pathogenic bacteria and mycotoxins and other "scavenger" functions; provide energy; meet the needs of physiological behaviors such as chewing. Soluble dietary fiber has a variety of physiological functions, including the prevention of car...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14A23K10/12A23K10/37A23K10/30A23K50/30C12R1/69C12R1/125C12R1/72
CPCC12N1/20C12N1/16C12N1/14A23K10/12A23K10/37A23K10/30A23K50/30Y02P60/87
Inventor 陆黎明胡承镇邓莉萍徐骏邓奕妮黄来妹刘雪连范彧贠桂玲
Owner 漳州大北农农牧科技有限公司
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