Preparation method of urea sensor with Janus structure artificial cell mode

Abstract

The invention discloses a preparation method of a urea sensor with a Janus structure artificial cell model, which belongs to the field of catalytic detection. The method comprises the following steps:adding a urease aqueous solution into a mixed solution of tributyrin and oleic acid, oscillating and performing standing to prepare a urease-based artificial cell model; preparing a phospholipid solution, adding the phospholipid solution into 20-40 [mu] L of glyceryl triacetate, oscillating and performing standing for 30 min to obtain an artificial cell model based on phospholipid; and oscillating the two settled artificial cell models to obtain two uniformly mixed micro-emulsions, and performing standing to remove a supernatant, thereby obtaining the Janus structure artificial cell model with the urea sensing function. According to the invention, the urea concentration is detected by using the urea sensor of the Janus structure artificial cell model; the method is high in selectivity, simple to operate, convenient to carry and transport, free of special instruments, short in detection time (less than 1 minute) and good in data reproducibility, 1000 times of measurement can be realized by one-time preparation (1mL of artificial cell model), and the linear detection range is 0.1 mg / mL-10mg / mL.

Description

technical field

[0001] The invention belongs to the field of catalytic detection, and in particular relates to a preparation method of a urea sensor with a Janus structure artificial cell model. Background technique

[0002] In the process of catabolism of the body's substances, the deamination of amino acids produces ammonia, which is synthesized into urea through the ornithine cycle in the liver, and the urea is transported to the kidneys through the blood and excreted through the urine. Urea is one of the final products of amino acid catabolism in the body, so measuring the urea content in the blood can understand the renal function of the body. The determination method of urea can be divided into two categories: one is the direct method, urea reacts directly with a certain reagent to determine its product, the most common is the diacetyl monoxime method, which utilizes diacetyl to condense with urea under strong acid conditions The red 4,5-dimethyl-2-oximidazole compoun...

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