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New strain marine bacteria XAAS-72 and applications in plant antibiosis and growth promotion

A technology for promoting plant growth and fungus. It is applied in the directions of plant growth regulators, microorganism-based methods, and chemicals for biological control. , Wide application value, the effect of reducing the content

Active Publication Date: 2020-04-21
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Although there are many studies on growth-promoting microbial resources in my country, in the microbial control industry, the strains used for many years are Bacillus, Paenibacillus, etc. , there are problems such as single strains and unstable application effects, and it is urgent to screen new strains with high activity and stable functions to promote the vigorous development of the microbial fertilizer industry

Method used

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  • New strain marine bacteria XAAS-72 and applications in plant antibiosis and growth promotion
  • New strain marine bacteria XAAS-72 and applications in plant antibiosis and growth promotion
  • New strain marine bacteria XAAS-72 and applications in plant antibiosis and growth promotion

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Isolation, screening and identification of Pontibacter sp.XAAS-72 CGMCC No.16594

[0029] (1) Separation and screening:

[0030] Soil samples of salicornia rhizosphere were collected in Manas area, Xinjiang, and microorganisms were isolated and purified by gradient dilution method. Weigh 10g of the soil sample, put it into 100mL sterile water, add sterilized glass beads, shake at 200rpm, 30°C for 20min to fully disperse the sample. Using standard serial dilutions, dilute with sterile water to make 10 -2 -10 -5 concentrated diluent. Take 100 μL of the dilutions of each concentration, apply the conventional coating method on a solid medium plate containing 1 / 3MB, and place it in a constant temperature incubator at 30°C for cultivation. After 48 hours, after colonies grow on the plate, pick a single colony on the plate for purification and culture. For the purified single colony, transfer to TSA medium slant containing 2% NaCl and save it for later use.

...

Embodiment 2

[0050] Example 2: Determination of the antagonistic and growth-promoting functions of Pontibacter sp.XAAS-72 CGMCC No.16594

[0051] (1) Antagonism function determination method: using the double-layer plate method, 3 strains of antagonistic indicator bacteria Pseudomonas, Erwinia, and Agrobacterium were coated on the bottom medium, and poured on the medium containing about 45 ° C containing 2 The 1 / 3MB medium of % NaCl was used to make the second layer. After the second layer was solidified, the strain Pontibacter sp.XAAS-72 CGMCC No.16594 was planted on the plate, incubated at 30°C for 3-7 days, observed and recorded Antagonism of strains against indicator bacteria.

[0052](2) Determination method of phosphorus-dissolving function: inoculate Pontibacter sp.XAAS-72 CGMCC No.16594 on phosphorus-dissolving (inorganic phosphorus and organic phosphorus) bacterial culture medium, and observe the size of the transparent circle after culturing at 30°C for one week.

[0053] (3) De...

Embodiment 3

[0059] Example 3: Preparation of Pontibactersp.XAAS-72 CGMCC No.16594 bacterial agent

[0060] After Pontibacter sp.XAAS-72 CGMCC No.16594 was inoculated on 1 / 3BD solid medium containing 2% NaCl for activation, it was transferred to 100mL 2% NaCl 1 / 3BD liquid medium and cultured at 180rpm at 30°C for 48h. Obtain the seed solution. The seed solution was inoculated in the fermentation medium at a ratio of 2%, and cultured at 30° C. and 200 rpm for 96 hours. The fermented liquid was diluted with sterilized water to make a total concentration of live bacteria of 1×10 7 The bacterial agent of CFU / mL is used for the subsequent plant growth promotion experiment.

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Abstract

The invention discloses a new strain Pontibactersp.XAAS-72 in marine bacteria, and applications in plant antibiosis and growth promotion. According to the invention, a strain of Pontibactersp.XAAS-72CGMCC No.16594 in marine bacteria is screened out from plural batches of strains separated from rhizosphere soil of salicornia europaea in a Sinkiang Manasi region; the separated strain has the characteristics of antagonism of various bacterial pathogens, salt resistance, growth promotion, phosphate solubilization, indoleacetic acid secretion, ACC deaminase production and the like; the results ofstrain classification and scientific identification show that the strain belongs to a new strain Pontibacter sp in marine bacteria; and the strain liquid fermentation liquid preparation can be appliedto promotion of plant growth and prevention and treatment of plant diseases, has remarkable and stable technical effects, and has wide application value in the technical field of microbial strain application.

Description

technical field [0001] The invention relates to the technical field of microbial strains and their applications, in particular to the technical field of a strain of the genus Marine Fungi and its application in plant antibacterial growth promotion. Background technique [0002] When plants grow normally, they are susceptible to the influence of bacteria and other environments. Under suitable environmental conditions, bacteria can interfere and destroy the metabolism and normal development of plants through propagation and reproduction, induce plant disease, and eventually weaken plant growth. Lower yields and lower quality. Especially in continuous cropping land, due to the imbalance of flora in the soil, bacterial diseases commonly occur, such as streak disease of wheat and rice in common crops, soft rot of potatoes, bacterial angular spot of cucumber in vegetables, and bacterial wilt of eggplant Disease and soft rot, etc., are caused by pathogenic bacteria. Common pathog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/11A01P1/00A01P21/00C12R1/01
CPCC12R2001/01C12N1/205Y02A40/10
Inventor 张志东朱静顾美英楚敏艾尼江·尔斯满唐琦勇宋素琴孙建古丽尼沙·沙依木
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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