Moisturizing and repairing facial mask containing active polypeptides and preparation method thereof

A technology for active polypeptides and facial masks, which is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of single facial mask function, poor absorption of active ingredients, dull skin, pigmentation, wrinkles, etc. Metabolism, preparation method is environmentally friendly, the effect of small molecular weight

Inactive Publication Date: 2020-04-28
上海中匡生物科技有限公司
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AI-Extracted Technical Summary

Problems solved by technology

Frequent use of facial masks can keep people's skin moisturized and shiny, but with the deterioration of the environment and the growth of age, people's skin will appear dullness, spots, wrinkles and other skin problems. Simple hydratio...
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Method used

The present inventor finds through long-term research, selects the mixing of biological sugar gum-1 and mannan as the preparation raw material of mask fluid, can guarantee the stability of mask viscosity, can also improve the stability of active polypeptide simultaneously, promote polypeptide The penetration ability of the mask ensures that the mask can penetrate deep into skin care, improve the suppleness of the skin, make the skin smooth and delicate, and achieve the anti-aging effect. The possible reason is that if mannan is added alone, its viscosity is small and its fluidity is large. If bio-glucose gum-1 is added, it can not only solve the above-mentioned problem of low viscosity of mannan, but at the same time, bio-glucose gum-1 can also It can deeply repair the skin and improve the skin's immunity. In addition, the use of bio-glucose-1 and mannan together can enhance the stability of the polypeptide. The possible reason is that the linear high-molecular sugar and the branched high-molecular sugar form a network of hydrogen bonds with the polypeptide, thereby The natural conformation of the polypeptide is stabilized, so the activity of the biological polypeptide can be maintained for a long time and the degradation of its structure can be prevented.
The present inventor finds through research for a long time, adopt macromolecular hyaluronic acid and small molecule hyaluronic acid to mix as preparation raw material, can improve the moisturizing effect of facial film; Possible reason is that the hyaluronic acid of small molecule can go deep into cell The inner layer quickly hydrates with the cells to maintain the moisture of the cells, while the macromolecular hyaluronic acid has poor permeability, and it can gradually form a moisture-rich film on the surface of the skin, which can lock in moisture while also retaining moisture. Delivers moisture to the skin, keeping the skin supple and lubricated for a long time.
The present inventor finds through research for a long time, carries out high-temperature sterilization and degreasing to pig small intestinal submucosa matrix first, then cryogenic treatment cycle 3-5 times, then immediately placed in -196 ℃ of ultra-low temperature pulverization, can be pulverized into certain Granular size particles, and can be hydrolyzed into small molecule peptides to the maximum extent, without affecting the biological activity of small molecule peptides. High-speed emulsification of powder particles, filtration, and freeze-drying can further obtain small molecular polypeptides with better quality. Finally, irradiation treatment is used to play a sterilizing effect a...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Abstract

The invention relates to the technical field of cosmetics, in particular to a moisturizing and repairing facial mask containing active polypeptides and a preparation method thereof. The moisturizing and repairing facial mask containing active polypeptides comprises a facial mask carrier and facial mask liquid coated on the facial mask carrier, wherein the facial mask liquid comprises the followingpreparation raw materials in parts by weight: 0.05 to 0.5 part of hyaluronic acid, 1 to 8 parts of water-soluble small molecules, 0.1 to 1 part of preservatives, 0.01 to 0.5 part of a thickening agent, 0.01 to 2 parts of active peptides, 1 to 3 parts of polysaccharides, 0.5 to 2 parts of saccharide isomeride, 0.1 to 0.5 part of a neutralizer and the balancing water to reach the total of 100 parts. The moisturizing and repairing mask containing the active polypeptide has the effects of tightening the skin, locking and replenishing the water, moisturizing the skin, removing free radicals and keeping the skin tender and white, and can relieve aging of the skin caused by factors such as ultraviolet irradiation, environmental pollution, age increase and the like and improve the skin tissue regeneration ability after long-time use.

Application Domain

Technology Topic

PeptideSkin tender +10

Image

  • Moisturizing and repairing facial mask containing active polypeptides and preparation method thereof
  • Moisturizing and repairing facial mask containing active polypeptides and preparation method thereof
  • Moisturizing and repairing facial mask containing active polypeptides and preparation method thereof

Examples

  • Experimental program(4)
  • Comparison scheme(7)

Example Embodiment

[0070] In a most preferred embodiment, the preparation method of the active polypeptide comprises the following steps:
[0071] (1) Clean the submucosa matrix of the porcine small intestine, divide it into strips with a length of 10 cm and a width of 2 cm, and process it at 120° C. for 30 minutes to obtain tissue samples;
[0072] (2) Immediately place the above-mentioned tissue samples at -80°C for 24 hours, then thaw the tissue samples with warm water at 30°C at a ratio of 10:1, and repeat this cycle for 3-5 times to obtain the thawed tissue samples;
[0073] (3) Physically pulverize the above-mentioned thawed tissue samples at -196°C to prepare powder particles with a particle size below 200 mesh;
[0074] (4) Mix the above powder particles in pure water at a mass ratio of 4:100, and emulsify at 21000 rpm for 5 minutes to obtain a slurry;
[0075] (5) Heat the above slurry to 100°C for 30 minutes, add an appropriate amount of pure water after cooling and heat to 100°C for 20 minutes, then filter with nylon gauze with a pore size of 30 μm, collect the filtrate, and freeze-dry to obtain a powder semi-finished product;
[0076] (6) The above powder semi-finished product is irradiated with 30KGy to obtain the active polypeptide.
[0077] After a long period of research, the inventors found that the submucosal matrix of the pig small intestine was subjected to high-temperature sterilization and degreasing, followed by cryogenic treatment for 3-5 times, and then immediately placed at -196°C for ultra-low temperature crushing, which can be crushed into particles of a certain size , and can be hydrolyzed into small molecule polypeptides to the greatest extent without affecting the biological activity of small molecule polypeptides. High-speed emulsification of powder particles, filtration, and freeze-drying can further obtain small molecular polypeptides with better quality. Finally, irradiation treatment is used to play a sterilizing effect again, thereby enhancing the quality of the small molecule polypeptide complex. The molecular weight is continuously reduced, and finally the effect of transdermal absorption can be achieved.
[0078] polysaccharide
[0079] The polysaccharide of the present invention is a polysaccharide high molecular carbohydrate composed of sugar chains bound by glycosidic bonds and at least more than 10 monosaccharides.
[0080]In one embodiment, the polysaccharide is selected from the group consisting of gum arabic, xanthan gum, dehydroxanthan gum, sclerotinia, hydrolyzed sclerotinia, carrageenan, locust bean gum, gellan gum, pectin, Bio-glucose gum-1, bio-glucose gum-2, bio-glucose gum-3, bio-glucose gum-4, bio-glucose gum-5, β-glucan, glycosyl trehalose, tremella polysaccharide, pullulanase polysaccharide, Alcaligenes polysaccharides, polyamino acid polysaccharide condensates, zymosan, soluble proteoglycans, aloe vera leaf polysaccharides, tuberose polysaccharides, euglenophyllum polysaccharides, senna angustifolia polysaccharides, mannan A mixture of one or more of sugars, fructooligosaccharides, fructans, succinoglycans, glycosphingolipids, glycogen, sucrose, and starch.
[0081] In a preferred embodiment, the polysaccharide is a mixture of bioglucan gum-1 and mannan.
[0082] In a preferred embodiment, the mass ratio of bio-glucose gum-1 to mannan is 1:(0.5-1.5).
[0083] In a most preferred embodiment, the mass ratio of bio-glucose gum-1 to mannan is 1:1.
[0084] The bio-glucose gum-1 is a linear bio-polysaccharide, which has a strong ability to bind water, and has a good water absorption effect even in a low-humidity environment. Evaporating, while having immediate and long-lasting moisturizing effects, dermatological application not only has moisturizing and soothing effects, but also gives cosmetics a soft and silky skin feel. At the same time, it has sensitizing properties as it promotes the removal of blunt macrophages from the infected site by competitively inhibiting MIF (macrophage motility inhibitory factor). In addition, it also has a pleasant skin feel, can regulate the user's emotions, and give the user a pleasant sensory experience and a feeling of happiness.
[0085] The mannan has a very strong water-locking and moisturizing ability, exerts an efficient skin care and moisturizing function, and has no sticky feeling, and has anti-aging and anti-oxidation effects.
[0086] The mannan was purchased from Shanghai Huixin Biotechnology Co., Ltd., with the brand name SC.
[0087] The bio-glucose gum-1 was purchased from Youke Biotechnology Co., Ltd., and the brand name is AQUATECH-S.
[0088] After long-term research, the inventors found that choosing the mixture of biological sugar gum-1 and mannan as the raw material for the preparation of the mask liquid can ensure the stability of the mask viscosity, and can also improve the stability of the active polypeptide and promote the penetration of the polypeptide. , so as to ensure that the mask can penetrate into the skin care, improve the suppleness of the skin, make the skin smooth and delicate, and achieve the anti-aging effect. The possible reason is that if mannan is added alone, its viscosity is small and its fluidity is large. If bio-glucose gum-1 is added, it can not only solve the above-mentioned problem of low viscosity of mannan, but at the same time, bio-glucose gum-1 can also It can deeply repair the skin and improve the skin's immunity. In addition, the use of bio-glucose-1 and mannan together can enhance the stability of the polypeptide. The possible reason is that the linear high-molecular sugar and the branched high-molecular sugar form a network of hydrogen bonds with the polypeptide, thereby The natural conformation of the polypeptide is stabilized, so the activity of the biological polypeptide can be maintained for a long time and the degradation of its structure can be prevented.
[0089] sugar isomers
[0090] The sugar isomers described in the present invention, also known as water-locking magnets, are colorless or yellowish transparent liquids, a natural humectant extracted from sugarcane sugar complexes, and assist moisture in binding horns in the stratum corneum protein to achieve moisturizing effect; it is also a good water retaining agent and emollient, which has the ability to repair epidermal cells and enhance skin hydration. Widely used in various skin and hair cosmetics. In skin cosmetics, it has good skin humidity adjustment function and long-term moisturizing effect, and the effect can be maintained until the cutin naturally peels off, moisturizing and relieving irritation.
[0091] Neutralizer
[0092] In one embodiment, the neutralizing agent is triethanolamine.
[0093] The second aspect of the present invention provides the preparation method of the moisturizing and repairing facial mask containing active polypeptide, comprising the following steps:
[0094] (1) Mix water, hyaluronic acid, water-soluble small molecules, preservatives, and thickeners, and stir evenly at 75-95°C;
[0095] (2) Cool down to 50-70°C, add neutralizing agent, and stir evenly again;
[0096] (3) Cool down to 40-50°C, add active polypeptides, polysaccharides, and sugar isomers, and stir evenly to obtain a mask liquid;
[0097] (4) The above-mentioned facial mask liquid is compounded on a facial mask carrier, and then a pearlescent paper is attached to the facial mask liquid to obtain a moisturizing and repairing facial mask containing polypeptides.
[0098] In a preferred embodiment, the preparation method of the moisturizing and repairing facial mask containing active polypeptide comprises the following steps:
[0099] (1) Add water, hyaluronic acid, water-soluble small molecules, preservatives, and thickeners into the emulsification pot in sequence, stir and dissolve completely at 85°C, and cool down after becoming a uniform liquid;
[0100] (2) Cool down to 60°C, add neutralizing agent, stir evenly again, and cool down;
[0101] (3) Cool down to 45°C, add active polypeptides, polysaccharides, and sugar isomers, stir evenly, take a sample at 40°C, filter, and discharge to obtain a mask liquid;
[0102] (4) The above-mentioned facial mask liquid is compounded on a facial mask carrier, and then a pearlescent paper is attached to the facial mask liquid to obtain a moisturizing and repairing facial mask containing polypeptides.

Example Embodiment

[0105] Example 1
[0106] Embodiment 1 provides a kind of moisturizing and repairing facial mask containing active polypeptides, comprising a facial mask carrier and a facial mask liquid coated on the facial mask carrier, and the facial mask liquid includes the following preparation raw materials in parts by weight: 0.15 parts of hyaluronic acid, water-soluble small molecule 5.5 parts, 0.5 parts of preservatives, 0.2 parts of thickeners, 0.1 parts of active polypeptides, 2 parts of polysaccharides, 1 part of sugar isomers, 0.13 parts of neutralizers, and make up to 100 parts of water.
[0107] The facial mask carrier is a spunlace non-woven facial mask paper.
[0108] The water is deionized water.
[0109] The hyaluronic acid is a mixture of macromolecular hyaluronic acid and small molecular hyaluronic acid.
[0110] The molecular weight of the macromolecular hyaluronic acid is 1.3 million Da; the molecular weight of the small molecular hyaluronic acid is 10,000 Da.
[0111] The mass ratio of the macromolecular hyaluronic acid to the small molecular hyaluronic acid is 0.5:1.
[0112] The water-soluble small molecule is a mixture of glycerin, 1,3-butanediol and 1,2-hexanediol.
[0113] The mass ratio of glycerin, 1,3-butanediol and 1,2-hexanediol is 3:2:0.5.
[0114] The preservative is p-hydroxyacetophenone.
[0115] The thickener is a mixture of hydroxyethyl cellulose and carbomer.
[0116] The mass ratio of the hydroxyethyl cellulose and carbomer is 1:1.8.
[0117] The active polypeptide is a sterile composite polypeptide rich in type III collagen sequence.
[0118] The molecular weight of the active polypeptide is 4000-8000Da.
[0119] The preparation method of the active polypeptide comprises the following steps:
[0120] (1) Clean the submucosa matrix of the porcine small intestine, divide it into strips with a length of 10 cm and a width of 2 cm, and process it at 120° C. for 30 minutes to obtain tissue samples;
[0121] (2) Immediately place the above-mentioned tissue samples at -80°C for 24 hours, then thaw the tissue samples with warm water at 30°C at a ratio of 10:1, and repeat this cycle for 3-5 times to obtain the thawed tissue samples;
[0122] (3) Physically pulverize the above-mentioned thawed tissue samples at -196°C to prepare powder particles with a particle size below 200 mesh;
[0123] (4) Mix the above powder particles in pure water at a mass ratio of 4:100, and emulsify at 21000 rpm for 5 minutes to obtain a slurry;
[0124] (5) Heat the above slurry to 100°C for 30 minutes, add an appropriate amount of pure water after cooling and heat to 100°C for 20 minutes, then filter with nylon gauze with a pore size of 30 μm, collect the filtrate, and freeze-dry to obtain a powder semi-finished product;
[0125] (6) The above powder semi-finished product is irradiated with 30KGy to obtain the active polypeptide.
[0126] The polysaccharide is a mixture of biological sugar gum-1 and mannan.
[0127] The mass ratio of the bio-glucose gum-1 to mannan is 1:1.
[0128] The mannan was purchased from Shanghai Huixin Biotechnology Co., Ltd., with the brand name SC.
[0129] The bio-glucose gum-1 was purchased from Youke Biotechnology Co., Ltd., and the brand name is AQUATECH-S.
[0130] The preparation method of the moisturizing and repairing mask containing active polypeptide comprises the following steps:
[0131] (1) Add water, hyaluronic acid, water-soluble small molecules, preservatives, and thickeners into the emulsification pot in sequence, stir and dissolve completely at 85°C, and cool down after becoming a uniform liquid;
[0132] (2) Cool down to 60°C, add neutralizing agent, stir evenly again, and cool down;
[0133] (3) Cool down to 45°C, add active polypeptides, polysaccharides, and sugar isomers, stir evenly, take a sample at 40°C, filter, and discharge to obtain a mask liquid;
[0134] (4) The above-mentioned facial mask liquid is compounded on a facial mask carrier, and then a pearlescent paper is attached to the facial mask liquid to obtain a moisturizing and repairing facial mask containing polypeptides.

Example Embodiment

[0135] Example 2
[0136] Embodiment 2 is basically the same as Example 1, the only difference is that it includes a facial mask carrier and a facial mask liquid coated on the facial mask carrier, and the facial mask liquid includes the following preparation raw materials in parts by weight: 0.1 part of hyaluronic acid, 2 parts of water-soluble small molecules 0.1 part of preservative, 0.1 part of thickener, 0.05 part of active polypeptide, 1 part of polysaccharide, 0.5 part of sugar isomer, 0.1 part of neutralizer, and make up to 100 parts of water.
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PUM

PropertyMeasurementUnit
Particle size<= 200.0mesh
Aperture30.0µm
Molecular weight4000.0 ~ 8000.0
tensileMPa
Particle sizePa
strength10

Description & Claims & Application Information

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