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Preparation method for tissue engineering cartilage scaffold

A tissue engineering and cartilage technology, which is applied in the technical field of chemical cross-linking, can solve the problems of decreased mechanical strength, inability of dynamic mechanical culture of cells, and reduced mechanical retention of scaffolds, and achieves enhanced mechanical properties, controllable degradation rate, The effect of reducing the degradation time

Active Publication Date: 2020-04-28
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen-based bioscaffolds currently have limitations, including the decline in mechanical strength and mechanical retention in vivo, and the inability of dynamic mechanical culture of scaffolds and cells in vitro.

Method used

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  • Preparation method for tissue engineering cartilage scaffold

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preparation example Construction

[0046] A kind of preparation method of tissue engineering cartilage scaffold of the present invention, see figure 1 As shown, its implementation steps are as follows:

[0047] Step (1): Preparation of silk protein solution

[0048] The "silk protein" is a natural fibrous protein derived from silkworm cocoons; the silk protein solution is prepared by the following method:

[0049] 1) 10g NaHCO 3 Dissolve in 2 liters (L) of ultrapure water to make 0.5% NaHCO 3 solution, heated to boiling;

[0050] 2) 10g shredded cocoons are put into NaHCO 3 In solution (boiling), cook for 1 hour (h);

[0051] 3) Take out the silk that has been boiled for 1 hour, and rinse it 5 times in ultrapure water;

[0052] 4) Take out the silk, unfold it on a clean tray, place it in an oven at 45°C, and dry it;

[0053] 5) Dissolve 8g of dried silkworm cocoons in 100ml of LiBr (9.3mol / L), cook in a water bath at 40°C (3h) until dissolved;

[0054] 6) Filter the dissolved silk protein solution into ...

Embodiment ( 1

[0066] Embodiment (one): the preparation of silk protein solution

[0067] The "silk protein" is a natural fibrous protein derived from silkworm cocoons; the silk protein solution is prepared by the following method:

[0068] 1) 10g NaHCO 3 Dissolve in 2L ultrapure water to make 0.5% NaHCO 3 solution, heated to boiling;

[0069] 2) 10g shredded cocoons are put into NaHCO 3 In the solution (boiling), cook for 1h;

[0070] 3) Take out the silk that has been boiled for 1 hour, and rinse it 5 times in ultrapure water;

[0071] 4) Take out the silk, unfold it on a clean tray, place it in an oven at 45°C, and dry it;

[0072] 5) Dissolve 8g of dried silkworm cocoons in 100ml of LiBr (9.3mol / L), cook in a water bath at 40°C (3h) until dissolved;

[0073] 6) Filter the dissolved silk protein solution into the Erlenmeyer flask with double-layer gauze;

[0074] 7) Put the filtered silk protein solution into a dialysis bag with a molecular weight cut-off of 8000-14000, and dialyze...

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Abstract

The invention provides a preparation method for a tissue engineering cartilage scaffold. The method comprises the following implementation steps: step (1), preparing a silk protein solution; and step(2), preparing the tissue engineering cartilage scaffold: preparing the silk protein scaffold. Through the above steps, the tissue engineering scaffold with an internal through-hole structure, biodegradability, good biocompatibility, and realization of large strain multiple cyclic compression is prepared, and a novel material system is provided for dynamic culture of cartilage tissue engineering cells and the scaffold in vitro.

Description

technical field [0001] Aiming at the unsatisfactory long-term effect of current clinical articular cartilage tissue repair, the present invention relates to a technology combining silk protein freeze-thaw gel-chemical cross-linking, that is, silk protein (SF), chemical cross-linking agent ethylene glycol diglycidyl ether The blend solution of (EGDE) and initiator tetramethylethylenediamine (TEMED) is placed in a low temperature environment, and the water in the blend solution is used to crystallize to form ice, and SF, EGDE and TEMED are squeezed around the ice, so that the silk The protein solution is in a high concentration state, so that physical cross-links are formed between SF molecules, and chemical cross-links are formed between SF molecules and EGDE molecules, thereby promoting the gelation of the SF blend solution; then the ice is thawed at room temperature , and finally get the silk cartilage scaffold. The invention belongs to the technical field of cartilage tissu...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50A61L27/56A61L27/58C12N5/077
CPCA61L27/227A61L27/50A61L27/56A61L27/58A61L2430/06C12N5/0655C12N2533/56C08L89/00
Inventor 管娟吴素君毛智南毕雪薇
Owner BEIHANG UNIV
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