Saccharomycopsis fibuligera with oxidation resistance and skin whitening effect and application of Saccharomycopsis fibuligera

A technology for buckling, laminating and whitening effects, applied in the biological field, can solve the problems that natural skin care products cannot meet consumer demand, and the development effort is not enough, and achieves the promotion of tyrosinase inhibition rate, good effect, whitening and wrinkle removal effect. Effect

Active Publication Date: 2020-05-01
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the fact that the current domestic market does not have a lot of development efforts in the integration of probiotic fermentation into skin care...

Method used

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  • Saccharomycopsis fibuligera with oxidation resistance and skin whitening effect and application of Saccharomycopsis fibuligera
  • Saccharomycopsis fibuligera with oxidation resistance and skin whitening effect and application of Saccharomycopsis fibuligera
  • Saccharomycopsis fibuligera with oxidation resistance and skin whitening effect and application of Saccharomycopsis fibuligera

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The antioxidation of embodiment 1 yeast extracellular active substance is measured

[0031] (1) Take the wine and medicine sample to be tested, add sterile water, dilute 10 times in a gradient, oscillate, take the supernatant and spread it on a solid medium, cultivate it for 12-48 hours, and obtain a single colony as the strain to be screened;

[0032] (2) Under sterile conditions, take the bacterial strain to be screened, inoculate it in a multi-hole cell culture plate containing a liquid medium, and activate it for 3 generations before use;

[0033] (3) After centrifuging the activated bacterial solution at 4000r / min for 2min, take the supernatant for later use;

[0034] (4) Carry out the pre-test, take 2 mL of DPPH solution, add a small amount of sample solution to it, when adding the sample, gradually add less and then more, mix while adding, and observe the fading of the solution, when the color of the solution basically fades, record The sample volume of the next...

Embodiment 2

[0041] Example 2 Determination of the tyrosinase inhibitory activity of yeast extracellular active substances

[0042] (1) Take 30 strains to be tested, centrifuge the activated bacterial solution at 4000r / min for 2min, and take the supernatant for use.

[0043] (2) Configure the required solution according to the table below:

[0044] Table 2. Determination of tyrosinase inhibition rate experimental sample addition table

[0045] Unit (mL) C1 C2 T1 T2 PBS buffer 1.5 2.0 1.0 1.5 sample 0 0 0.5 0.5 Tyrosinase 0.5 0 0.5 0 total capacity 2.0 2.0 2.0 2.0

[0046] Add C1, C2, T1, and T2 according to the above table and bathe in a water bath at 37°C for 10 minutes, add 1 mL of dopa solution and bathe in a water bath at 37°C for 4 minutes, and immediately put it into a spectrophotometer at 475nm to measure the absorbance at the 5th minute , Calculate the inhibition rate of the sample to tyrosinase activity T (%)=1-(T1-T2) / (C1-C2...

Embodiment 3

[0050] Antioxidant activity, tyrosinase inhibition rate determination of the active substance in the yeast cell of embodiment 3

[0051] (1) Take the strains 1-13 to be tested, centrifuge the activated bacterial liquid at 4000r / min for 2min, and put the centrifuged supernatant (that is, the mixture of MEB medium and extracellular secretion) in a sterile operating table completely empty;

[0052] (2) Add 0.9ml of sterile water, use a micro-vortex mixer to mix it completely, cover the EP tube, and centrifuge again at low temperature and low speed for a short period of time. The purpose of this centrifugation is to clean up the remaining EP tube The mixed solution of MEB medium and extracellular secretion, repeat this experimental step 2-3 times, until the medium in the EP tube is completely washed;

[0053] (3) Find a small glass beaker, add an appropriate amount of ice-water mixture and a small foam orifice to it, open the EP tube and insert it into the small foam orifice, aft...

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Abstract

The invention provides Saccharomycopsis fibuligera with the collection number of CCTCC M 2019324. Tests prove that the Saccharomycopsis fibuligera CCTCC M2019324 has DPPP free radical scavenging rateof an extracellular supernatant is 81.9% and a tyrosinase inhibition rate of 68.1%. The extracellular supernatant of Saccharomycopsis fibuligera CCTCC M2019324 is fermented with a medicinal plant as asubstrate, and a fermentation liquid of the extracellular supernatant has a better acne propionibacterium inhibition effect by detection. Meanwhile, the medicinal plant fermentation liquid can also be applied to preparation of cosmetics, and a prepared product of the plant fermentation liquid containing the e Saccharomycopsis fibuligera CCTCC M2019324 can effectively scavenge free radicals, promote skin renewal and reduce synthesis of melanin and has very good application prospects.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a strain of Saccharomycopsis fibuligera with antioxidant properties and whitening effects and an application thereof. Background technique [0002] Skin care products have always been a hot topic in consumption, and consumers' desire for excellence in skin care has promoted the vigorous development of the market. It can be seen from the market trend in recent years that the growth of natural plant cosmetics has always exceeded that of synthetic cosmetics globally, and China is the fastest growing country for natural plant cosmetics. In addition to the use of foreign popular plant ingredients by international brands, It has also begun to intervene in the biological field. The current popular SK-II fairy water has a good whitening effect because it is rich in yeast fermentation liquid. Microbial fermentation technology is the process of using the metabolism of micr...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P1/02A61K8/9728A61Q19/00A61Q19/02A61Q19/08A61P17/10C12R1/645
CPCC12P1/02A61K8/9728A61Q19/00A61Q19/02A61Q19/08A61P17/10A61K2800/782C12R2001/645C12N1/145
Inventor 陈臣刘洋田怀香于海燕刘政陈彬景艳郑丹蔚
Owner SHANGHAI INST OF TECH
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