Compound microbial agent for ecological restoration and preparation method of compound microbial agent

A compound microbial inoculant and ecological restoration technology, applied in the field of microorganisms, can solve the problems of not taking into account the concentration of dissolved oxygen, pathogenic bacteria and biochemically degraded organic matter in water, failing to achieve water ecological restoration of polluted water body treatment, and failing to achieve the purpose of water body treatment, etc. Achieve the effect of improving dissolved oxygen in water, wide application range and good removal ability

Active Publication Date: 2020-05-01
ZHENGZHOU UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Application number 201510666377.5 discloses a composite microbial water purification agent and its preparation method. Although it can reduce the content of COD, ammonia nitrogen and sulfide in water, it does not take into account the concentration of dissolved oxygen, pathogenic bacteria and biodegradable organic matter in water, so the agent does not Not suitable for all bodies of water
[0004] The water environment is a complex environment. When microorganisms play their role in removing pollutants, they need the coexistence of multiple conditions. Relying on a single microbial flora cannot achieve the purpose of water body governance, and removing a single pollutant cannot achieve the governance of polluted water bodies and the improvement of water ecology. repair

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  • Compound microbial agent for ecological restoration and preparation method of compound microbial agent

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Embodiment 1

[0031] The present embodiment provides a kind of preparation method of the composite microbial inoculum used for ecological restoration, comprising the following steps:

[0032] (1) Rhodopseudomonas, purple sulfur bacteria, green sulfur bacteria, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Candida, Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus amyloliquefaciens, Pseudomonas Monomonas, Nitrobacter victoria, Nitrosococcus, denitrifying bacteria, Chlorella pyrenoidosa, Chlorella ellipsoides, and Spirulina gigantea are expanded and cultivated through their respective culture media, and then the corresponding microbial liquid is obtained through a fermenter and algae liquid, through a plate and frame filter press to obtain microbial cells and algae, and then use glycerin and glycine as protective agents to obtain microbial powder and algal powder by spray drying, and mix microbial powder and algal powder in a certain proportion to obtain microbial Bac...

Embodiment 2

[0037] The composite microbial bacterial agent of the present embodiment comprises 87 parts of microbial bacterial algae powder, 5 parts of microbial growth aids, and 8 parts of biological enzymes; wherein,

[0038] The composition of microbial algae powder is: Rhodopseudomonas powder 1wt%, purple sulfur bacteria powder 1wt%, green sulfur bacteria powder 3wt%, brewer’s yeast powder 2wt%, killing yeast powder 5wt%, candida powder 3wt% %, Bacillus subtilis powder 15wt%, Bacillus licheniformis powder 10wt%, Bacillus cereus powder 5wt%, Bacillus amyloliquefaciens powder 2wt%, Pseudomonas stutzeri powder 13wt%, Nitrobacter victoria powder 5wt%, Nitrosococcus powder 5wt%, denitrifying bacteria powder 10wt%, chlorella pyrenoidosa powder 5wt%, chlorella ellipsoid powder 5wt%, spirulina gigantea powder 10wt%;

[0039] The composition of the microbial growth aid is: sodium bicarbonate 10wt%, potassium chloride 10wt%, magnesium sulfate 5wt%, iron sulfate 5wt%, calcium nitrate 5wt%, isomalt...

Embodiment 3

[0042] The composite microbial bacterial agent of the present embodiment comprises 81 parts of microbial bacteria and algae powder, 9 parts of microbial growth aids, and 10 parts of biological enzymes; wherein,

[0043] The composition of microbial algae powder is: Rhodopseudomonas powder 1wt%, purple sulfur bacteria powder 2wt%, green sulfur bacteria powder 2wt%, brewer’s yeast powder 3wt%, killing yeast powder 4wt%, candida powder 3wt% %, Bacillus subtilis powder 10wt%, Bacillus licheniformis powder 12wt%, Bacillus cereus powder 6wt%, Bacillus amyloliquefaciens powder 3wt%, Pseudomonas stutzeri powder 15wt%, Nitrobacter victoria powder 5wt%, Nitrosococcus powder 5wt%, denitrifying bacteria powder 5wt%, chlorella pyrenoidosa powder 10wt%, chlorella ellipsoid powder 8wt%, spirulina gigantea powder 6wt%;

[0044] The composition of the microbial growth aid is: sodium bicarbonate 8wt%, potassium chloride 8wt%, magnesium sulfate 5wt%, iron sulfate 5wt%, calcium nitrate 6wt%, isom...

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Abstract

The present invention relates to a compound microbial agent for ecological restoration and belongs to the technical field of microorganisms. The compound microbial agent comprises microbial flora, algae, microbial promoters and biological enzymes; wherein the microbial flora comprises photosynthetic bacteria, yeasts, heterotrophic aerobic bacteria, pseudomonas stutzeri, nitrobacter winogradsky, nitrococcus and denitrifying bacillus; the algae comprises chlorella pyrenoidosa, chlorella ellipsoidea and spirulina maxima; the microbial promoters comprise sodium bicarbonate, potassium chloride, magnesium sulfate, ferric sulfate, calcium nitrate, isomalt oligosaccharide, fructooligosaccharide, inulin and xylooligosaccharide; and the biological enzymes are one or more of cellulase, protease, amylase, pectinase and lipase. The compound microbial agent can be applied to a variety of water body environments, removes a variety of pollutants, at the same time has advantages of good pollutant removal effect and quick effect, and has great potential for promotion and application.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microbial agent for ecological restoration and a preparation method thereof. Background technique [0002] With the needs of my country's ecological civilization construction, the treatment of black and odorous water bodies and polluted water bodies has received great attention. As an eco-friendly, efficient, and non-secondary pollution-free ecological water treatment technology, microbial bacterial agents have played a great role in river and lake governance and ecological restoration. [0003] However, the microbial composition of the current microbial inoculum is relatively single. Due to the different water environmental conditions, pollution degrees and pollutant types in different water bodies, not all microorganisms in the microbial inoculum can become the dominant flora in the water body to exert their full potential. Its pollutant removal function. A...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/18C12N1/12C12N9/50C12N9/42C12N9/26C12N9/20C02F3/34C02F3/02C12R1/01C12R1/645C12R1/38C12R1/865C12R1/72C12R1/125C12R1/10C12R1/09C12R1/07C12R1/89
CPCC12N1/20C12N1/16C12N1/18C12N1/12C12N9/2437C12Y302/01004C12N9/50C12N9/2411C12N9/20C12Y301/01003C02F3/34C02F3/347C02F3/348C02F3/02C02F2303/02Y02W10/10
Inventor 高镜清郭晗曾磊媛张敬申尚娜陈勇高健磊
Owner ZHENGZHOU UNIV
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