Preparation method of electrochemical molecular imprinting sensor based on chitosan oligosaccharide derivative as functional monomer
A technology of functional monomers and molecular imprinting, which is applied in the direction of material electrochemical variables, scientific instruments, and material analysis through electromagnetic means, can solve the problem of low specificity, achieve high-sensitivity detection, reduce background current, and improve sensitivity Effect
Active Publication Date: 2020-05-19
YANGZHOU POLYTECHNIC INST
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However, in these methods, because curcumin analogs have the same chromophore, the specificity is often not high. In some studies, it is impossible to give an accurate judgment on the stability of its metabolites and itself, so some specificity is developed. , high-sensitivity detection methods are necessary
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[0027] 1. Preparation of molecularly imprinted sensors.
[0028] The preparation method of the molecularly imprinted sensor in this example is as follows: figure 1 shown.
[0029] 1. Thermal polymerization:
[0030] Add 3mg of curcumin and 6mg of N-(N′-pentenoyl-glutamyl)-oligochitosan to 5mL of DMF and H 2 In a mixed solvent composed of O, ultrasonically dissolve at room temperature, and then add 50 mg of cross-linking agent ethylene glycol dimethacrylate (EGDMA), and 1.5 mg of initiator azobisisobutyronitrile (AIBN). After standing still for 12 hours, purify with nitrogen for at least 10 minutes to remove dissolved oxygen and obtain a mixed solution. Pipette 5 μl of the mixed solution onto the surface of a clean glassy carbon electrode and cover with a clean cover glass. Then place it in a 65°C oven and heat it for 12h, and after removing the cover glass, a layer of transparent polymer film is formed on the surface of the glassy carbon electrode.
[0031] 2. Preparation...
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The invention discloses a preparation method of an electrochemical molecular imprinting sensor based on a chitosan oligosaccharide derivative as a functional monomer and relates to the technical fieldof electrochemical sensors, curcumin is used as a template molecule, N-(N '-pentenoyl-glutamine acyl)-chitosan oligosaccharide is used as a functional monomer oligomer, ethylene glycol dimethacrylateis used as a cross-linking agent, azodiisobutyronitrile is used as an initiator, and a cross-linking agent is used as an initiator. Firstly, a curcumin molecularly imprinted membrane is formed on thesurface of a glassy carbon electrode through thermal polymerization; according to the method, curcumin is taken as a raw material and then subjected to electrolytic secondary polymerization in a solution containing N, N-methylene bisacrylamide and ammonium persulfate, recognition sites are left on the surface of an electrode after elution with an eluent, ferrocene methanol is taken as an electroactive probe, and the curcumin is indirectly detected by measuring the current change of charge transfer reaction of the ferrocene methanol in the recognition process.
Description
technical field [0001] The invention relates to the technical field of electrochemical sensors. Background technique [0002] Curcumin is a chemical substance extracted from the rhizomes of some plants in Zingiberaceae and Araceae. It is a rare phenolic compound with a diketone structure in the plant kingdom. Curcumin is an orange-yellow crystalline powder with a slightly bitter taste and is insoluble in water. It is mainly used as a pigment in the food industry. Modern medical research shows that curcumin not only has the effects of lowering blood fat, anti-tumor, anti-inflammatory, choleretic, and anti-oxidation, but also has certain curative and preventive effects on many diseases, such as diabetes and drug-resistant tuberculosis. Therefore, the determination of curcumin content in biologically active health products and food spices is an important analytical task in pharmacological research and food industry. [0003] At present, the detection method of curcumin is mai...
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IPC IPC(8): G01N27/30G01N27/48C08F251/00C08F216/36C08J9/26C08L51/02
CPCG01N27/308G01N27/48C08F251/00C08J9/26C08J2351/02C08J2201/0422C08F216/36
Inventor 高庆徐嘉琪郭双华王富花
Owner YANGZHOU POLYTECHNIC INST