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Extraction method of green prickleyash alkaloids

An extraction method, the technology of green pepper, applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of low extraction rate, slow phase separation speed of two-phase system, and separation effect of total alkaloids and substances to be separated Instability and other problems, to achieve the effect of fast phase separation, improved purity and performance, and stable separation effect

Active Publication Date: 2020-06-02
营山椒宝宝花椒有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pure alkaloid reference substance used in this method still has the defect that the extraction rate is not high during actual application.
In addition, the phase separation speed of the two-phase aqueous system is slow, and the separation effect of the total alkaloids and the substances to be separated is unstable, thus affecting the purity and performance of the total alkaloids

Method used

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  • Extraction method of green prickleyash alkaloids
  • Extraction method of green prickleyash alkaloids
  • Extraction method of green prickleyash alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 100 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=6.0 with 18wt% of alkyl alcohol ether glycoside AEG30, 22wt% of dipotassium hydrogen phosphate, 1.5wt% of potassium chloride and the rest of deionized water; then at 40°C Mix well, and let stand at constant temperature. According to the solid-liquid ratio of 1:12g / mL, weigh 100 mesh green pepper powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 60°C to obtain an extract. The microwave extraction conditions are: microwave power 260W; extraction time 20min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Use the polysulfone ultrafiltration membrane with a molecular weight ...

Embodiment 2

[0054] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 80 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=6.5 with 20wt% of alkyl alcohol ether glycoside AEG30, 20wt% of dipotassium hydrogen phosphate, 1wt% of potassium chloride and the rest of deionized water; then at 45°C Mix well and let stand at constant temperature. According to the solid-liquid ratio of 1:10g / mL, weigh 80 mesh green pepper powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 65°C to obtain an extract. The microwave extraction conditions are: microwave power 240W; extraction time 18min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Purify the upper phase at an operating pressure of 0.4 MPa and room temper...

Embodiment 3

[0056] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 120 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=5.5 with 16wt% of alkyl alcohol ether glycoside AEG30, 24wt% of dipotassium hydrogen phosphate, 2wt% of potassium chloride and the rest of deionized water; then at 35°C Mix well and let stand at constant temperature. According to the solid-liquid ratio of 1:14g / mL, weigh 120 mesh green pepper powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 55°C to obtain an extract. The microwave extraction conditions are: microwave power 280W; extraction time 22min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Purify the upper phase at an operating pressure of 0.2 MPa and room temp...

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Abstract

An extraction method of green prickleyash alkaloids, disclosed in the invention, comprises the following steps: carrying out microwave extraction on green prickleyash powder and an aqueous two-phase extraction solvent; filtering the obtained extract solution, standing, and splitting phases; carrying out ultrafiltration and nanofiltration operations on the upper phase; and concentrating and spray-drying to obtain an alkaloid extract, wherein the aqueous two-phase extraction solvent is selected from an alkyl alcohol ether glucoside / dipotassium hydrogen phosphate aqueous two-phase system with thepH value of 5.5-6.5. According to the method, total alkaloids with a high proportion can be obtained, and the phase splitting speed is high, so that the purity and the performances of the alkaloid extract are improved.

Description

technical field [0001] The invention belongs to the technical field of biochemical products, and relates to a green and environment-friendly extraction method of green pepper, more specifically, an extraction method of green pepper alkaloids. Background technique [0002] Green Zanthoxylum bungeanum is a plant of the genus Zanthoxylum Bungeanum Maxim, and generally said Zanthoxylum bungeanum refers to the dried fruit of this type of plant. There are about 250 species of this genus in the world, distributed in tropical and subtropical regions such as Asia, Africa, South America and Oceania. my country is one of the countries with the largest pepper planting area in the world, mainly distributed in Sichuan, Chongqing, Gansu, Shandong and Hebei provinces. [0003] The most common use of green Zanthoxylum bungeanum is as a condiment. It is a favorite spice of our people and is known as one of the eight great flavors. In addition to the peel can be used for food and medicine, o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G5/00
CPCC07G5/00
Inventor 王汪杨华忠周静熊定伟史亮武碧先
Owner 营山椒宝宝花椒有限责任公司
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