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Method for testing ecotoxicity of disinfection by-products

A technology of disinfection by-products and ecotoxicity, which is applied in compound screening/testing, measuring devices, instruments, etc., can solve the problems of high requirements and lower reliability of results, so as to improve safety, facilitate promotion, and meet the needs of cultivation. Simple operation effect

Inactive Publication Date: 2020-06-16
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For in vitro experiments, such as the CHO cell test system commonly used in the toxicity test of disinfection by-products in drinking water, it has the advantages of high sensitivity and close relationship with human cells. Promote the use in water plants; for in vivo experiments, most of the current studies use freshwater organisms as model organisms (for example, freshwater daphnia magna), which are susceptible to interference from external factors such as increased salinity caused by water sample concentration, reducing the credibility of the results Spend

Method used

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  • Method for testing ecotoxicity of disinfection by-products
  • Method for testing ecotoxicity of disinfection by-products
  • Method for testing ecotoxicity of disinfection by-products

Examples

Experimental program
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Embodiment 1

[0029] First prepare the artificial seawater: add sea salt to the ultrapure water, adjust the salinity to 35±1‰, and finally filter it through a glass fiber filter membrane with a pore size of 0.22 μm, put it in a brown bottle for temporary storage, and prepare it now. Then prepare the water sample to be tested: weigh a certain mass of IAM (iodoacetamide) and dissolve it in the prepared artificial seawater, prepare 4 groups of water samples to be tested with different IAM (iodoacetamide) concentrations, each group is prepared 500mL.

[0030] Hatching of Artemia: Take 3-5g of anhydrous Artemia freeze-dried eggs and put them into a 1500mL beaker filled with 1200mL seawater, seal the top of the beaker with aluminum foil, put it in a constant temperature water bath at 26±1°C, and blow air with a small air pump Perform gas storm incubation for 12 hours; immediately after the hatching is over, randomly pick out the first instar Artemia with a Pasteur pipette and place it in a 150 mm...

Embodiment 2

[0037] First prepare the artificial seawater: add sea salt to the ultrapure water, adjust the salinity to 35±1‰, and finally filter it through a glass fiber filter membrane with a pore size of 0.22 μm, put it in a brown bottle for temporary storage, and prepare it now. Then prepare the water sample to be tested: weigh a certain mass of IAA (iodoacetic acid) and dissolve it in the prepared artificial seawater, prepare 10 groups of water samples to be tested with different IAA (iodoacetic acid) concentrations, and prepare 500 mL for each group.

[0038] Hatching of Artemia: Take 5g of anhydrous freeze-dried eggs of Artemia and put them into a 1500mL beaker filled with 1200mL of seawater. Air-incubated for 10 hours; immediately after the hatching was over, the first instar Artemia was randomly picked out with a Pasteur pipette and placed in a 150mm crystallization dish filled with sufficient seawater.

[0039] Cultivation of Artemia: Put the first instar Artemia in a light incuba...

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Abstract

The invention discloses a method for testing ecotoxicity of disinfection by-products, and the method comprises the following steps: incubating and culturing artemia to obtain second-instar artemia larvae; putting artemia larvae into a water sample containing disinfection by-products, and respectively obtaining the survival rate of the artemia larvae through acute toxicity and chronic toxicity exposure, so as to calculate the toxicity of the water sample to artemia. The method is wide in coverage and covers both acute toxicity and chronic toxicity, and a test water sample can be a single substance solution and an actual disinfection water sample; the used artemia is high in sensitivity, convenient to hatch and simple to culture; the raw materials are easy to obtain and low in price; the method has the advantages of low requirements on the culture environment, clear toxicity endpoint, and convenience in popularization in sewage and reuse water plants, and is a simple, sensitive and economic toxicity test method.

Description

technical field [0001] The invention relates to the technical fields of environmental health and water quality analysis, in particular to a method for testing the ecotoxicity of disinfection by-products. Background technique [0002] Multiple pressures such as climate change, industrial and agricultural development, and population growth have led to global water resource stress. Recycled water that can be used in industries, agriculture, and other fields has become an important alternative water resource to address the growing demand for water resources; disinfection is usually As the final step of wastewater reuse to reduce the risk of disease from reclaimed water. In addition, the chlorine disinfection process is also used by some advanced sewage treatment plants as a means of degrading refractory pollutants. However, when chlorine kills pathogenic microorganisms, it also produces a variety of disinfection by-products (DBPs), which will bring certain ecological risks to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00G01N33/186A61K49/0008G01N33/18
Inventor 楚文海徐祖信栾鑫淼
Owner TONGJI UNIV
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