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Preparation method of thixotropic hydrogel for 3D biological printing

A bioprinting and thixotropic technology, applied in biochemical equipment and methods, microbial determination/inspection, processing and manufacturing, etc., can solve the problems of poor biocompatibility, long recovery time, poor formability, etc., and achieve excellent injection. The effect of low extrusion force and excellent formability

Active Publication Date: 2020-06-23
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thixotropic hydrogel materials can take advantage of their characteristics of "thinning under force and recovery after force removal" (You Chen, a Yihan Wang, Qian Yang, et al. A novel thixotropic magnesium phosphate-based bioink with excellent printability for application in 3Dprinting, journal of materials chemistry B, 2018, 6, 4502-4513), but the existing thixotropic materials are often chemically synthesized materials, and the biocompatibility is not excellent; and the literature The reported thixotropic materials used in the biomedical field have a long recovery time after thixotropy (You Chen, a Yihan Wang, Qian Yang, et al. Anovelthixotropic magnesium phosphate-based bioink with excellent printability for application in 3D printing, journal of materials chemistry B,2018,6,4502-4513), leading to poor formability after printing, it is difficult to continue printing on the printing layer faster, which limits its application in 3D bioprinting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a thixotropic hydrogel for 3D bioprinting, comprising the following steps:

[0027] A, stock solution preparation: gelatin powder, tannic acid powder and sodium alginate powder are respectively dissolved in deionized water to obtain a gelatin solution with a concentration of 6.0wt.% respectively; a tannic acid solution with a concentration of 0.25wt.%; 3.0wt.% sodium alginate solution;

[0028] B. Synthesis of gelatin-tannic acid binary complex: add the gelatin solution and tannic acid solution prepared in step A into the reactor at a ratio of 1:1, and stir magnetically for 2 hours in a water bath at 40°C to obtain gelatin - tannic acid binary complex solution;

[0029] C, preparation of gel: the sodium alginate solution configured in step A is added to the gelatin-tannic acid binary complex solution of B step, wherein the sodium alginate solution and the gelatin-tannic acid binary complex solution The volume ratio is 3:1, and the color of the m...

Embodiment 2

[0031] A method for preparing a thixotropic hydrogel for 3D bioprinting, comprising the following steps:

[0032] A, stoste preparation: fibrinogen powder, tea polyphenol powder and chitosan powder are respectively dissolved in deionized water, respectively to obtain a concentration of 3wt.% fibrinogen solution; concentration of 0.10wt.% tea polyphenol Solution; concentration is 2.0wt.% chitosan solution;

[0033] B. Synthesis of fibrinogen-tea polyphenol binary complex: add the fibrinogen solution and tea polyphenol solution prepared in step A into the reactor at a ratio of 5:1, and stir magnetically in a water bath at 45°C 1h, obtain the fibrinogen-tea polyphenol binary complex solution;

[0034] C. Preparation of gel: Add the chitosan solution configured in step A to the fibrinogen-tea polyphenol binary complex solution in step B, wherein the chitosan solution and the fibrinogen-tea polyphenol binary The volume ratio of the complex solution is 2:1, and the color of the solu...

Embodiment 3

[0036] A method for preparing a thixotropic hydrogel for 3D bioprinting, comprising the following steps:

[0037] A, stock solution preparation: respectively dissolve fibrin powder, caffeic acid powder and gellan gum powder in deionized water to obtain respectively a fibrin solution with a concentration of 10.0wt.%; a caffeic acid solution with a concentration of 0.75wt.%; 8.0wt.% gellan gum solution;

[0038] B. Synthesis of fibrin-caffeic acid binary complex: Add the fibrin solution and caffeic acid solution prepared in step A into the reactor at a ratio of 10:1, and stir magnetically for 3 hours in a water bath at 50°C to obtain fibers Protein-caffeic acid binary complex solution;

[0039] C, preparation of gel: the gellan gum solution configured in step A is added to the fibrin-caffeic acid binary complex solution of B step, wherein the gellan gum solution and the fibrin-caffeic acid binary complex solution The volume ratio is 4:1, and the color of the magnetically stirr...

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Abstract

The invention relates to a preparation method of a thixotropic hydrogel for 3D biological printing, which comprises the following steps: A, dissolving protein powder, polyphenol powder and polysaccharide powder in deionized water to respectively obtain protein solutions with the concentration of 3.0-10.0 wt.%; a polyphenol solution having a concentration of 0.10 to 0.75 wt.%; and a polysaccharidesolution having a concentration of 2.0 to 8.0 wt.%; B, adding the protein solution and the polyphenol solution prepared in the step A into a reactor according to a ratio of (1-10): 1, and magneticallystirring for 1-3 hours under a water bath condition of 40-50 DEG C so as to obtain a protein-polyphenol binary compound solution; and C, adding a polysaccharide solution into the compound solution obtained in the step B according to a volume ratio of (2-4): 1, and magnetically stirring the solution under a water bath condition of 40-50 DEG C until the solution is uniform in color and is not layered, thereby obtaining the product. The hydrogel prepared by the method is used as a 3D biological printing material, and has excellent biocompatibility and injectivity.

Description

technical field [0001] The invention relates to a preparation method of thixotropic hydrogel for 3D bioprinting. Background technique [0002] 3D bioprinting is a model based on medical image conversion design. Under the precise control of the computer, the bioprinting "ink" (biocompatible materials and cells) is printed layer by layer, and a three-dimensional structure and microenvironment similar to that in the body is constructed in vitro. The three-dimensional structure and microenvironment obtained by 3D bioprinting provide a bionic three-dimensional cell culture condition, in which the cells are properly cultured and finally cultivated into functional species for in vivo replacement and regeneration as well as in vitro drug screening and disease research. Tissue / Organ. At present, there are three common 3D bioprinting methods: inkjet printing, laser-assisted and extrusion (Li J, Chen M, Fan X, et al.Recent advances in bioprinting techniques: approaches, applications a...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08L5/00C08L5/04C08L5/08C08L5/02C08L5/12C08H1/00C12Q1/02A61L27/20A61L27/22A61L27/52B29C64/106B33Y70/00
CPCC08J3/075C08H1/00G01N33/5008A61L27/20A61L27/22A61L27/52B29C64/106B33Y70/00C08J2389/00C08J2405/00C08J2405/04C08J2405/08C08J2405/02C08J2405/12C08J2489/00C08J2305/00C08J2305/04C08J2305/08C08J2305/02C08J2305/12
Inventor 屈树新崔荣伟熊雄陈园园李茂红王生龙方麒博翁杰
Owner SOUTHWEST JIAOTONG UNIV