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Oxygen-containing nano diamond for antibacterial treatment and preparation method thereof

A nano-diamond and oxygen-containing technology, applied in the field of medical antibacterial materials, can solve the problems of being unsuitable for clinical medical applications, unable to destroy plaque biofilm, and not having peroxidase-like activity, achieving low cost and easy operation , The effect of simple preparation process

Pending Publication Date: 2020-07-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nanodiamonds prepared by this method have no purification treatment, only have the ability to simply inhibit the growth of bacteria, do not have peroxidase-like activity, cannot destroy the formed plaque biofilm, and are not suitable for clinical medical applications

Method used

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  • Oxygen-containing nano diamond for antibacterial treatment and preparation method thereof
  • Oxygen-containing nano diamond for antibacterial treatment and preparation method thereof
  • Oxygen-containing nano diamond for antibacterial treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 oxygen-containing nano-diamond

[0029] Oxygen-containing nano-diamonds containing specific oxygen groups were synthesized by chemical water bath method. Disperse nano-diamond powder with a diameter of 5-10nm in H 2 SO 4 with HNO 3 In the mixture, H 2 SO 4 with HNO 3 The volume ratio was 3:1, heated to 90°C, and continued to react for 24 hours. After the reaction was completed, sodium hydroxide solution was added to the sample for neutralization and cooling. The above samples were dialyzed in a dialysis bag with a cut-off molecular weight of 3500 kDa. Then, the dialysis product was dispersed in a 0.1 mol / mL HCl solution, and heated at 90° C. for 2 hours. Finally, dialyzed again and the product freeze-dried to obtain nanodiamonds (O-NDs) with abundant and specific oxygen-containing groups (transmission electron microscopy images see figure 1 ), the oxygen content on its surface is as figure 2 shown.

Embodiment 2

[0030] Example 2: Detection of enzyme activity of oxygen-containing nanodiamonds

[0031] The absorbance value (625nm) of the O-NDs / TMB system was detected by a UV spectrophotometer. In the experiment, at 25°C, O-NDs was added to an acetate buffer solution (pH 4.0) with a reaction volume of 25mM; before recording the absorbance, Add 1mMTMB to start the reaction; measure the O-NDs at 652nm / TMB / H within 200 seconds 2 o 2 The absorbance value changes within the system, and the peroxidase activity of O-NDs was analyzed using steady-state kinetics. Experimental results such as image 3 , O-NDs in H 2 o 2 In the presence of TMB, it can catalyze the blue reaction of TMB, showing good peroxidase-like activity.

Embodiment 3

[0032] Embodiment 3: Cytotoxicity test detection of oxygen-containing nanodiamond

[0033] Take the gingival fibroblasts grown in the logarithmic phase of the third generation, digest them with 1mL 0.25% trypsin, and divide them according to 6×10 3 Inoculate into a 24-well plate at a concentration of 1 / well and place in CO 2 Constant temperature incubator (5% CO 2 , 95% air, saturated humidity, 37°C) for 24 hours, after changing the medium, add O-NDs, O-NDs concentrations were 0μg / mL, 25μg / mL, 50μg / mL, 100μg / mL, 300μg / mL, 500μg / mL and cell-free group (blank group). After culturing in a constant temperature incubator for 24 hours, 10 μL of CCK-8 solution was added to each well and incubated in an incubator for 1.5 hours under dark conditions. 100 μL per well was transferred to a 96-well plate, and the absorbance value was detected at 450 nm using a microplate reader. The experimental results showed that O-NDs were co-cultured with cells for 24 hours, and no concentration-d...

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Abstract

The invention discloses oxygen-containing nano diamond for antibacterial treatment and a preparation method thereof, and belongs to the technical field of medical antibacterial materials. The diameterof the oxygen-containing nano diamond is 5 to 10nm, the surface groups are carboxyl, carbonyl and hydroxyl, and the surface oxygen content is 15-20%. The preparation method comprises the following steps: dispersing the nano-diamond into a mixture of H2SO4 and HNO3, heating, adding the obtained sample into a sodium hydroxide solution, cooling, dialyzing, dispersing the product into hydrochloric acid, heating, dialyzing again, and the like. The preparation process is simple and convenient, easy to operate and low in cost, and the prepared oxygen-containing nano-diamond has good peroxidase-likeactivity and has a good destructive effect on plaque biofilms formed by periodontal pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of medical antibacterial materials, and in particular relates to an oxygen-containing nano-diamond for antibacterial treatment, a preparation method and application thereof. Background technique [0002] Periodontitis, which affects approximately 10-15% of the adult population worldwide, is an oral infectious disease caused by periodontal pathogens that exist as plaque biofilms and lead to the destruction of tooth supporting tissues. Plaque biofilms consist of clusters of bacteria coated with extracellular macromolecules that firmly adhere to the surface of teeth and periodontal tissues. Biofilms that cause periodontitis, under the action of toxic species (such as Porphyromonas gingivalis), produce numerous virulence factors, which directly or indirectly lead to the destruction of periodontal tissue by modulating the host inflammatory response. [0003] Current approaches against periodontitis-biofilm are l...

Claims

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Application Information

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IPC IPC(8): C01B32/28B82Y40/00A61K33/44A61P31/04A61P1/02
CPCC01B32/28B82Y40/00A61K33/44A61P31/04A61P1/02
Inventor 方蛟李红东刘钧松周延民王启亮成绍恒高楠
Owner JILIN UNIV
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