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A bacterial agent and its application for reducing the harm of mildew and mycotoxins in fermented feed

A technology of fermenting feed and mycotoxins, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of easy breeding of mold, and achieve the effect of reducing the total amount of mold, reducing the content, and fast growth and reproduction

Active Publication Date: 2021-01-08
南京固与生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned microbial fermented feed can promote the absorption of animals, it is still very easy to breed harmful microorganisms such as mold due to the large amount of semi-decomposed components

Method used

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  • A bacterial agent and its application for reducing the harm of mildew and mycotoxins in fermented feed
  • A bacterial agent and its application for reducing the harm of mildew and mycotoxins in fermented feed
  • A bacterial agent and its application for reducing the harm of mildew and mycotoxins in fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A method for preparing a microbial inoculant for reducing the mildew and mycotoxin hazards of fermented feed, comprising the following steps:

[0062] (1) Bacillus subtilis Bsbc01 was activated and cultured on LB solid medium plate at 37°C for 24 hours to obtain activated strains; then, the activated strains were inoculated in LB liquid medium for 18 hours with shaking at 37°C and 200rpm to obtain Seed liquid; then, the seed liquid is inoculated into the liquid medium with an inoculation amount of 6% by volume, and cultivated at 37°C for 24h to obtain a Bacillus subtilis bacterial liquid; then, add a protective agent in a proportion of 5%, and carry out at 112°C Spray-drying under wind temperature to obtain Bacillus subtilis dry powder;

[0063] Described expanded culture is that described liquid culture medium component is as follows, is weight percent:

[0064] Corn flour 2%, bean cake flour 3%, bran 2%, sodium citrate 0.5%, KH 2 PO 4 0.03%, K 2 HPO 4 0.01%, Ca...

Embodiment 2

[0083] The microbial inoculant that reduces the mildew and mycotoxin hazards of fermented feed as described in Example 1 is different in that each gram includes the following active ingredients:

[0084] Bacillus subtilis Bsbc01 2×10 11 CFU, Candida utilis 2 x 10 10 cfu, lactic acid bacteria 5×10 10 cfu, compound enzyme preparation 500U, glucose 0.2g, peptone 0.4g;

[0085] The compound enzyme preparation is composed of neutral protease, acid protease, amylase, cellulase and NSP compound enzyme; the enzyme activity ratio of neutral protease, acid protease, amylase, cellulase and NSP compound enzyme is 2:3 :2:1:2.

[0086] The lactic acid bacteria are Lactobacillus plantarum, Lactobacillus casei and Streptococcus lactis mixed according to the ratio of live bacteria = 1.5:2.5:1.

Embodiment 3

[0088] The microbial inoculant that reduces the mildew and mycotoxin hazards of fermented feed as described in Example 1 is different in that each gram includes the following active ingredients:

[0089] Bacillus subtilis Bsbc01 1×10 11 cfu, Candida utilis 1 x 10 10 cfu, lactic acid bacteria 5×10 10 cfu, compound enzyme preparation 100U, glucose 0.15g, peptone 0.35g;

[0090] The compound enzyme preparation is composed of neutral protease, acid protease, amylase, cellulase and NSP compound enzyme; the enzyme activity ratio of neutral protease, acid protease, amylase, cellulase and NSP compound enzyme is 2.5:2.5 :2.5:1:1.5.

[0091] The lactic acid bacteria are Lactobacillus plantarum.

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Abstract

The invention relates to a fungus agent for reducing mildew of fermentation feed and mycotoxin harm and application. The microbial agent for reducing mildew of fermentation feed and mycotoxin harm comprises the following effective components: Bacillus subtilis, Candida utilis, lactic acid bacteria, compound enzyme preparation, glucose and peptone. According to the present invention, Bacillus subtilis Bsbc01 and Candida utilis are used together for the first time to prepare the fungus agent, Candida utilis has the advantages of simple required nutrients, fast growth and reproduction, etc., canquickly consume the oxygen present in the feed gap and limit the growth and reproduction of mold, and at the same time, Candida utilis has the characteristics of high protein and vitamin B content, etc., which can increase the nutritional value of feed.

Description

technical field [0001] The invention belongs to the technical field of microbial fermented feed, and in particular relates to a bacterial agent for reducing the mildew and mycotoxin hazards of fermented feed and its application. Background technique [0002] With the rapid development of the animal husbandry industry, the shortage of feed, especially protein feed raw materials, has become a key factor restricting the further development of the animal husbandry industry. In the 1950s, scientific research found that adding a small amount of antibiotics and other substances to animal diets It can effectively improve the feed utilization rate of animals and promote the production performance of animals. This discovery has made a great contribution to the further rapid development of the animal husbandry industry. However, with the growth of time, especially problems such as drug-resistant bacteria and super bacteria People found that the harm of adding antibiotics in animal feed r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/12A23L5/20C12R1/125C12R1/72C12R1/25C12R1/46C12R1/245
CPCA23K10/12A23L5/28C12N1/16C12N1/20
Inventor 马成杨桂娟
Owner 南京固与生物有限公司
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