Unlock instant, AI-driven research and patent intelligence for your innovation.

Modification-free and fixation-free homogeneous-phase electrochemical detection method for organophosphorus pesticide

An organophosphorus pesticide and electrochemical technology, which is used in measuring devices, scientific instruments, and material analysis by electromagnetic means, can solve problems such as poor conductivity, and achieve the effects of low cost, good repeatability and mild reaction conditions.

Inactive Publication Date: 2020-07-03
QINGDAO AGRI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: zeolite imidazole metal-organic framework material 8 (ZIF-8), as a class of MOFs materials, is reported to be able to decompose rapidly under acidic conditions, and this property is mostly used in drug delivery and cancer treatment. characteristics for pesticide residue detection, which have not been reported so far
In addition, because most MOFs materials are poor in conductivity, the application of MOFs materials in the field of electrochemistry is relatively small.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modification-free and fixation-free homogeneous-phase electrochemical detection method for organophosphorus pesticide
  • Modification-free and fixation-free homogeneous-phase electrochemical detection method for organophosphorus pesticide
  • Modification-free and fixation-free homogeneous-phase electrochemical detection method for organophosphorus pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Feasibility Analysis Experiment for Detecting Organophosphorus Pesticides

[0030] In the present embodiment, select paraoxon pesticide (OP) to detect as the representative of organophosphorus pesticide, and the method for detecting organophosphorus pesticide of the present embodiment comprises the following steps:

[0031] (1) Dissolve 25mM zinc nitrate, 25mM 2-methylimidazole, and 80μM methylene blue in 30mL of methanol solution, age at room temperature for 24h without stirring, then collect the white precipitate by filtration, and wash the white precipitate with methanol times, and then vacuum-dried at room temperature to generate ZIF / MB composites;

[0032] (2) Mix 4000ng / mL paraoxon solution (or an equal volume of water) with 0.6U / mL acetylcholinesterase (AChE) solution, incubate at 37°C for 30min, then add 100mM acetylcholine chloride solution, React at 37°C for 150 minutes to obtain the reacted solution;

[0033] (3) Add 1mg / mL of the ZIF / MB composite...

Embodiment 2

[0035]Embodiment 2 Sensitive detection of linear relationship experiment of organophosphorus pesticides

[0036] In the present embodiment, select paraoxon pesticide (OP) to detect as the representative of organophosphorus pesticide, and the method for detecting organophosphorus pesticide of the present embodiment comprises the following steps:

[0037] (1) Dissolve 20mM zinc nitrate, 20mM 2-methylimidazole, and 150μM methylene blue in 20mL of methanol solution, age at room temperature for 18h without stirring, then collect the white precipitate by filtration, and wash the white precipitate with methanol times, and then vacuum-dried at room temperature to generate ZIF / MB composites;

[0038] (2) Mix 5, 10, 20, 100, 400, 1000, 2000, 4000ng / mL paraoxon solution with 0.8U / mL acetylcholinesterase (AChE) solution, incubate at 30°C for 20min, then add 120mM acetylcholine chloride solution, reacted at 30°C for 130min to obtain the reacted solution;

[0039] (3) Add 1mg / mL of the ZI...

Embodiment 3

[0042] Embodiment 3 detects the versatility verification experiment of multiple organophosphorus pesticides

[0043] In this embodiment, paraoxon, methapon, and chlorpyrifos are selected as representatives of organophosphorus pesticides, and a mixture of paraoxon and methapon is used as a representative of mixed pesticides for detection. The method for detecting organophosphorus pesticides in this embodiment includes The following steps:

[0044] (1) Dissolve 30mM zinc nitrate, 30mM 2-methylimidazole, and 40μM methylene blue in 40mL of methanol solution, age at room temperature for 30h without stirring, then collect the white precipitate by filtration, and wash the white precipitate with methanol times, and then vacuum-dried at room temperature to generate ZIF / MB composites;

[0045] (2) Paraoxon, methapon, chlorpyrifos at 2000ng / mL, and a mixture solution of paraoxon (1000ng / mL) and methapon (1000ng / mL) were mixed with 1.2U / mL of acetylcholinesterase ( AChE) solutions were ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a modification-free and fixation-free homogeneous-phase electrochemical detection method for an organophosphorus pesticide, and belongs to the technical field of pesticide residue detection. According to the invention, the high loading capacity of a metal-organic framework material on electroactive signal molecules and the inhibition effect of an organophosphorus pesticideon acetylcholin esterase are used as a basis, a zeolite imidazole metal-organic framework material (ZIF / MB) encapsulating methylene blue is synthesized through a simple one-step self-assembly method;and the ZIF / MB composite material is dissolved by utilizing the characteristic that the ZIF / MB composite material can be hydrolyzed under an acidic condition and combining the characteristics that acetylcholine esterase can catalyze acetylcholine to generate acetic acid and reduce the acidity of a system, encapsulated electrochemical signal molecules MB are released, and relatively strong diffusion current is generated. The organophosphorus pesticide can inhibit the activity of acetylcholin esterase, so that the MB release process is hindered, the linear relation between the organophosphorus pesticide concentration and the MB diffusion current is established, and simple and high-sensitivity detection of organophosphorus pesticide residues is realized.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a modification-free and fixation-free homogeneous electrochemical detection method for organophosphorus pesticides. Background technique [0002] Pesticides are a class of chemical agents widely used to prevent, treat pests, or regulate plant growth. Pesticides are widely used in people's production and life to ensure the quality and yield of crops, as well as to eliminate insects and moths in daily life. Due to the widespread and unreasonable use of pesticides, the problem of pesticide residues is inevitable. Pesticide residues have caused great damage to human health, ecological environment, and economic development. For example, through the enrichment of the food chain, pesticides remain in the human body and cannot be metabolized to cause pesticide poisoning. In mild cases, it will cause headaches and nausea, and in severe cases, it will lead to cancer, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 刘晓娟李秀媛李峰
Owner QINGDAO AGRI UNIV