Unlock instant, AI-driven research and patent intelligence for your innovation.

Quick dyeing reagent for albumen glue

A rapid staining and protein glue technology, applied in the field of protein dyes, can solve the problems of reducing protein staining efficiency, increasing protein staining costs, and affecting protein mass spectrometry detection, so as to increase the dyeing rate, improve mass spectrometry compatibility and dye solution sensitivity, and improve The effect of the dyeing effect

Inactive Publication Date: 2020-07-17
安徽环球基因科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Protein staining is an important step after protein gel electrophoresis. At present, the commonly used protein staining reagents in China are divided into organic reagent staining represented by Coomassie brilliant blue, silver staining, fluorescent staining and isotope color development. Coomassie brilliant blue staining method has Low cost and good compatibility with mass spectrometry. Traditional protein stains need to be cleaned, fixed, and decolorized to stain proteins. The whole process lasts for 1-2 hours, and sometimes even requires overnight treatment, which greatly reduces protein staining. Efficiency, and toxic reagents such as methanol are needed in the staining process, so it is necessary to use a fume hood or solvent treatment for protein staining, which increases the cost of protein staining. Traditional protein staining has low sensitivity to stained proteins and residual There is a large amount of methylation, which affects the detection of proteins by mass spectrometry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick dyeing reagent for albumen glue
  • Quick dyeing reagent for albumen glue
  • Quick dyeing reagent for albumen glue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A protein glue fast staining reagent, prepared by the following steps:

[0044] Step S1: Weigh the following raw materials in parts by weight: 0.5 parts of the first dyeing agent, 0.5 parts of the second dyeing agent, 10 parts of acetic acid, 10 parts of ethanol, and 3 parts of distilled water;

[0045] Step S2: adding the first dyeing agent and ethanol into the stirring tank, and stirring for 5-15 minutes at a rotating speed of 1000 r / min to obtain the first dyeing solution;

[0046] Step S3: adding the second dyeing agent and acetic acid into the stirring tank, and stirring for 5 minutes at a rotating speed of 1000-1500 r / min to obtain the second dyeing solution;

[0047] Step 43: Add the first dye solution prepared in step S2, the second dye solution prepared in step S3, and distilled water into the stirring tank, and stir for 3 minutes under the condition of a rotating speed of 1000 r / min to obtain rapid dyeing of protein glue Reagent products.

[0048] Described ...

Embodiment 2

[0058] A protein glue fast staining reagent, prepared by the following steps:

[0059] Step S1: Weigh the following raw materials in parts by weight: 0.8 parts of the first dyeing agent, 0.8 parts of the second dyeing agent, 15 parts of acetic acid, 13 parts of ethanol, and 4 parts of distilled water;

[0060] Step S2: Add the first dyeing agent and ethanol into the stirring tank, and stir for 10 minutes at a rotating speed of 1300 r / min to obtain the first dyeing solution;

[0061] Step S3: adding the second dyeing agent and acetic acid into the stirring tank, and stirring for 10 min at a rotating speed of 1300 r / min to obtain the second dyeing solution;

[0062] Step 43: Add the first dye solution prepared in step S2, the second dye solution prepared in step S3, and distilled water into the stirring tank, and stir for 4 minutes at a rotation speed of 1300 r / min to obtain rapid dyeing of protein glue Reagent products.

Embodiment 3

[0064] A protein glue fast staining reagent, prepared by the following steps:

[0065] Step S1: Weigh the following raw materials in parts by weight: 1 part of the first dyeing agent, 1 part of the second dyeing agent, 20 parts of acetic acid, 15 parts of ethanol, and 5 parts of distilled water;

[0066] Step S2: Add the first dyeing agent and ethanol into the stirring tank, and stir for 15 minutes at a rotating speed of 1500 r / min to obtain the first dyeing solution;

[0067] Step S3: adding the second dyeing agent and acetic acid into the stirring tank, and stirring for 15 minutes at a rotating speed of 1500 r / min to obtain the second dyeing solution;

[0068] Step 43: Add the first dye solution prepared in step S2, the second dye solution prepared in step S3, and distilled water into the stirring tank, and stir for 5 minutes at a rotation speed of 1500 r / min to obtain rapid dyeing of protein glue Reagent products.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quick dyeing reagent for albumen glue. The dyeing reagent is prepared from the following raw materials in parts by weight: 0.5 to 1 part of a first dyeing agent, 0.5 to 1 part of a second dyeing agent, 10 to 20 parts of acetic acid, 10 to 15 parts of ethanol and 3 to 5 parts of distilled water. The first dyeing agent molecule is a cationic coloring agent, and can be combined with carboxyl with negative charges on a protein chain so as to achieve a dyeing effect; the second dyeing agent molecule is an anionic coloring agent; under the condition that the second dyeing agent and the first dyeing agent are used together, the dyes exist in the form of anions and cations, so that dynamic balance is formed between the ionic dyes and the free dyes, the binding efficiencyof free dye molecules and proteins is further improved, the dyeing rate of the proteins is further improved, and the mass spectrum compatibility and dye liquor sensitivity of the proteins are improveddue to the mixed use of the two dyeing agents.

Description

technical field [0001] The invention belongs to the technical field of protein dyes, in particular to a rapid staining reagent for protein glue. Background technique [0002] Proteomics refers to taking all the proteins encoded by the genome as the research object, studying the composition and changing rules of proteins from the cellular level and the overall level, so as to deeply understand the various physiology and pathology of the organism. Compared with traditional protein research, proteomics Proteomics research embodies the characteristics of comprehensiveness, integrity, high-throughput, and large-scale. The research of proteomics plays an irreplaceable important role in the empirical analysis of the proteome that has completed the theoretical prediction of the genome project. More importantly, the protein Group analysis does not need to rely on genome research, and protein research is expected to find biological function clues of "orphan" genes without any homologo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/30C09B11/16C09B29/08
CPCC09B11/16C09B29/08G01N1/30G01N2001/302
Inventor 雍金贵李森张伦
Owner 安徽环球基因科技有限公司