Check patentability & draft patents in minutes with Patsnap Eureka AI!

Preparation method of plant polyphenol

A plant polyphenol and plant raw material technology, applied in the direction of organic chemistry, can solve the problems affecting the application range of plant polyphenols, the purity of plant polyphenols is not high enough, poor stability, etc., to achieve post-processing, high purity, good stability Effect

Pending Publication Date: 2020-07-24
SHANGHAI JAKA BIOTECH CO LTD
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of plant polyphenols prepared by conventional methods is not high enough and the stability is poor, especially the aqueous solution of plant polyphenols is prone to discoloration, precipitation and precipitation, which seriously affects the application range of plant polyphenols in the field of daily cosmetics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of plant polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of wrinkled papaya total polyphenols:

[0039] 1. Take 1 kg of dry sliced ​​wrinkled papaya (Chaenomeles speciosa), soak it in 5L of deionized water at room temperature for half an hour, add 2 g of cellulase and 0.3 g of pectinase, and extract twice in a water bath at 45°C. Each time 1h, the two extracts were combined (total 7.2L). Add the prepared ZTC-Ⅱ clarifying agent under the condition of stirring at 400r / min (add 100ml of type IIB first, then add 100ml of type IIA, the dosage of both IIB and IIA clarifying agent is 1.39% of the volume of the extract), stir well and then let stand for 5h , followed by a 200-mesh filter cloth and a Buchner funnel to obtain a 7L filtrate.

[0040] 2. Concentrate the above 7L filtrate to about 2L and add the sample to the polyamide chromatographic column (55*450mm), and control the outflow rate of the liquid at the lower end of the chromatographic column to about 20ml / min. After adding the sample, it was first e...

Embodiment 2

[0043] Preparation of total polyphenols in wrinkled papaya with different proportions of alkaline eluent:

[0044] Take 2 kg of dried flaky wrinkled papaya decoction pieces, soak them in 20 L of deionized water at room temperature for half an hour, add 4 g of cellulase and 0.5 g of pectinase, and extract in a water bath at 50°C for 1 hour to obtain 16.2 L of extract. Under the condition of 400r / min stirring, add the prepared ZTC-Ⅱ clarifying agent (add 180ml of type IIB first, then add 180ml of type IIA, the amount of both IIB and IIA clarifying agent is 1.1% of the volume of the extract), stir well and then stand for 4h , followed by a 200-mesh filter cloth and a Buchner funnel to obtain a filtrate of 16.1 L.

[0045] The above-mentioned 16.1L filtrate was concentrated to about 6L and divided into 6 parts on average, and loaded into 6 polyamide chromatographic columns (30*300mm) respectively, and the outflow rate of the liquid at the lower end of the chromatographic column wa...

Embodiment 3

[0049] A preparation method of wrinkled papaya total polyphenols:

[0050] Using the same method as in Example 2, the only difference is that 6.8L of the filtrate prepared in the same way as in Example 2 is taken, concentrated to about 1.5L, and then loaded into a D101 macroporous adsorption resin column (55*450mm) , control the outflow rate of the liquid at the lower end of the chromatographic column to about 15ml / min. After adding the sample, it was first eluted with 2 times column volume of water, then 3 times column volume of 30% methanol, and finally 90% methanol until the effluent was colorless. The 90% methanol eluted fraction (approximately 2.9 L) was collected.

[0051] The weight of the total polyphenols of the obtained wrinkled papaya is 14.1g, and the purity of the total polyphenols is 58.3% as tested by the Folin phenol method.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of plant polyphenol, and the method comprises the following steps: S1, carrying out ultrasonic or heating extraction on plant raw materials by using a certain amount of solvent under the action of a compound enzyme, and collecting an extracting solution; S2, adding a natural clarifying agent into the extracting solution obtained in the step S1, carryingout clarification treatment, filtering, and collecting filtrate; S3, purifying the filtrate obtained in the step S2 by using adsorption resin column chromatography; and S4, carrying out ultrafiltration on the purified filtrate, then carrying out nanofiltration, and removing the solvent from obtained nanofiltration trapped fluid to obtain the plant polyphenol. The method is especially suitable forextraction and purification of various plant tannin, flavanol, flavonoid aglycone, phenolic acid and other glycosyl-free polyphenol components, and the yield of the prepared glycosyl-containing polyphenol (such as flavonoid glycoside and stilbene glycoside) is low. The preparation method comprises the following five steps: compound enzyme-assisted extraction, clarification, column chromatography,ultrafiltration and nanofiltration. The plant polyphenol prepared by the method is high in purity, light in color and good in stability.

Description

technical field [0001] The invention relates to the extraction technology of plant active ingredients, belongs to the technical field of plant extraction, in particular to a method for preparing plant polyphenols, and in particular to a method for extracting and purifying plant polyphenols. Background technique [0002] Plant polyphenols in a broad sense are the general term for chemical components that exist in plants and contain polyphenolic hydroxyl structures, including tannins (also called tannins, which are divided into condensed tannins and hydrolyzed tannins), flavonoids, flavanols, and stilbenes , phenolic acids and other components. Plant polyphenols have various physiological activities such as anti-oxidation, anti-aging, antibacterial, anti-virus, anti-cancer, etc., and have been widely used in the fields of medicine, health food and cosmetics. Especially in the field of daily cosmetics, because plant polyphenols can prevent or effectively alleviate various skin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07G99/00
CPCC07G99/00
Inventor 辛英祥王新亮马毅刚齐秋月周利丹刘青卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com