Nanogold cage and preparation method thereof
A nano-gold and seed technology, which is applied in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems that have not existed, and achieve simple operation, high yield, and increased targeting. effect of treatment
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Embodiment 1
[0067] The present embodiment provides a kind of preparation method of nanometer gold cage, comprises the following steps:
[0068] Step 101: Take 200 μL of 25 mmol sodium citrate solution and 250 μL of 20 mmol chloroauric acid solution, mix them, add distilled water to dilute to 20 ml, stir at high speed for 15 min, add 600 μL of 10 mmol sodium borohydride solution at 4°C, and stir at high speed for 15 min , and then filtered through a 220nm polyethersulfone membrane to obtain a negatively charged nano-gold seed solution.
[0069] Step 102: Take 10 μL of 2 mmol HAuCl 4 4H 2 O, added to the negatively charged nano-gold seed solution prepared in step S1, and the pH value was adjusted to 8 to obtain a weak base solution.
[0070] Step 103: Preparation of β-benzyl aspartate (BLA):
[0071] Step 1031: Take a 500mL single-mouth eggplant-shaped bottle, take 100mL of anhydrous ether, add it to the single-mouth eggplant-shaped bottle, add 10mL of concentrated sulfuric acid while st...
Embodiment 2
[0109] The present embodiment provides a kind of preparation method of nanometer gold cage, comprises the following steps:
[0110] Step 101: Take 200 μL of 25 mmol sodium citrate solution and 250 μL of 20 mmol chloroauric acid solution, mix them, add distilled water to dilute to 10 ml, stir at high speed for 8 minutes, add 600 μL of 10 mmol sodium borohydride solution at 6°C, and stir at high speed for 12 minutes, Filter through a 220nm polyethersulfone membrane to obtain a negatively charged nano-gold seed solution.
[0111] Step 102: Take 10 μL of 2 mmol HAuCl 4 4H 2 O, added to the negatively charged nano-gold seed solution prepared in step S1, and the pH value was adjusted to 8 to obtain a weak base solution.
[0112] Step 103: Preparation of β-benzyl aspartate (BLA):
[0113] Step 1031: Take a 500mL single-mouth eggplant-shaped bottle, take 100mL of anhydrous ether, add it to the single-mouth eggplant-shaped bottle, add 10mL of concentrated sulfuric acid while stirrin...
experiment example 1
[0151] The present invention also characterizes the DOX-loaded nano-gold cage prepared by the preparation method of the above-mentioned Example 1. Human ovarian cancer cell line SKOV-3 cells were purchased from the Cell Bank of the Chinese Academy of Sciences in Shanghai.
[0152] Characterization method: combination therapy experiment (cell)
[0153] Take 1mL of different concentrations of DOX-loaded nano-gold cages in a 4mL glass bottle, irradiate with a near-infrared laser, collect temperature data with a thermocouple, and take pictures with an infrared thermal imager. PBS is the blank control group for this experiment.
[0154] SKOV-3 cells were treated with 1×10 4 The cell density per well was seeded in a 96-well plate at 37°C with 5% CO 2 cultured overnight in an incubator. Nano gold cages loaded with DOX (Au concentration of 1 mg·mL -1 ) were co-cultured with SKOV-3 cells for 6 hours, discarded the old medium, and washed once with PBS. The 808nm laser was irradiate...
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