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Antibacterial hydrogel and preparation method and application thereof

A hydrogel, deionized water technology, applied in capsule delivery, pharmaceutical formulation, pharmaceutical science, etc., can solve the problems of easy killing of normal cells, high cytotoxicity, fast release, etc., and achieve excellent biocompatibility and mechanical properties. Good performance and antibacterial performance

Active Publication Date: 2020-08-07
杭州口腔医院集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cytotoxicity of traditional silver-containing dressings is high, it is easy to kill normal cells in the human body, and there are problems such as rapid release.

Method used

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  • Antibacterial hydrogel and preparation method and application thereof
  • Antibacterial hydrogel and preparation method and application thereof
  • Antibacterial hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Preparation of sodium alginate microspheres (Alg): configure 20 mL of 2 wt % sodium alginate aqueous solution, stir well and set aside. Add the above-mentioned sodium alginate aqueous solution into a flask containing 100 mL of soybean oil, stir with a mechanical stirrer at a speed of 400 to 600 r / min (500 r / min in this example) for 1 h at 60 ° C, and then add 1 mL of Span 80 After emulsifying at a speed of 400-600r / min (500r / min in this embodiment) for 60min, add 40mL of calcium chloride solution with a concentration of 2%, and continue to stir for 60min. Then add 10 mL of isopropanone at 4° C., stir for 30 minutes, and then centrifuge at 4000 to 8000 r / min for 5 to 10 minutes at 25° C. (6000 r / min for 10 minutes in this example). Finally, it was alternately washed three times with isopropanol, ethanol and deionized water to remove the oil phase, and finally freeze-dried to obtain the sodium alginate microspheres (Alg).

Embodiment 2

[0040] Preparation of silver nanoparticles (AgNPs): (1) Dissolve 500 mg of silver nitrate in 20 mL of ethanol to obtain a dispersion, and store in the dark; (2) Dissolve 1 g of polyvinylpyrrolidone (PVP) in 20 mL of ethanol to obtain a For the original solution, place the reducing solution in a constant temperature water bath at 60°C to keep warm for later use; (3) Add the dispersion liquid to the reducing solution in the constant temperature water bath in the dark, and keep stirring the reducing solution during the addition. Along with the progress of the reaction, it can be observed that the color of the reaction solution gradually changes from colorless and transparent to golden yellow, and continues to deepen, and finally becomes dark red until the color becomes stable, and freeze-dried to obtain the silver nanometer (AgNPs ).

Embodiment 3

[0042] Preparation of sodium alginate microspheres loaded with silver nanoparticles (Alg@AgNPs): (1) 500 mg of silver nitrate was dissolved in 20 mL of ethanol to obtain a dispersion, and stored in the dark; (2) 1 g of polyvinylpyrrolidone (PVP) Dissolve in 20mL of ethanol to obtain a reducing solution, and place the reducing solution in a constant temperature water bath at 60°C to keep warm for later use; (3) In the dark, add 1.5g of sodium alginate microspheres prepared by the method in Example 1 to disperse (4) After centrifuging the reaction system of step (3), wash it with deionized water, and then centrifuge after ultrasonic vibration; repeat the operation 5 times; (5) disperse the reaction product in ethanol, and then Drop into the reducing solution in a constant temperature water bath, and stir the reducing solution continuously during the addition process. Along with the progress of the reaction, it can be observed that the color of the reaction solution gradually cha...

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Abstract

The invention discloses antibacterial hydrogel, which is prepared from the following components: methacrylated chitosan and antibacterial microspheres, and the antibacterial microspheres are sodium alginate microspheres loaded with nano-silver, wherein a mass ratio of the methacrylated chitosan to the antibacterial microspheres is 10: (0.1-2). According to the preparation method, the nano-silver is loaded into the sodium alginate microspheres, so that the slow release of the nano-silver is realized. Meanwhile, the sodium alginate microspheres loaded with the nano-silver are added into the methacrylated chitosan hydrogel to form microsphere-containing antibacterial hydrogel, so that the antibacterial property of the material is greatly improved, the cytotoxicity of the nano-silver is reduced, and the release period of the nano-silver is as long as 10 days or longer. The antibacterial hydrogel disclosed by the invention is safe, non-toxic and good in antibacterial property, shows appropriate swelling and degradation behaviors, has excellent biocompatibility and mechanical properties, is biodegradable and bioabsorbable, and also has injectability and a hemostatic function.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an antibacterial hydrogel and its preparation method and application. Background technique [0002] Since the first discovery of penicillin in 1928, antibiotics have been widely used in the field of antibacterial. With the development of public health and biomedical technology, many infections have been effectively suppressed or even conquered. However, the use of antibiotics has led to the emergence of resistant microbes that are very difficult to kill, leading to more than 13 million deaths from infectious diseases worldwide each year. Corresponding resistant bacteria, such as floxacin-resistant Enterococcus (K-1476) and methicillin-resistant Staphylococcus aureus (MRSA), emerged almost immediately after the emergence of advanced antibiotics. In addition, traditional antibiotics face other problems, such as solubility, overdose, and cytotoxicity. Therefore, an e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0023A61L26/0061A61L26/0066A61L26/008A61L26/009A61L2300/104A61L2300/404A61L2300/62A61L2400/04A61L2400/06C08L5/08
Inventor 王维倩林海燕周艳周健
Owner 杭州口腔医院集团有限公司
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