Method for producing an extract of cereal and method for processing this extract into beverage
A technology of extracts and grains, applied in biochemical equipment and methods, food science, malt preparation, etc., can solve energy consumption and other problems, achieve the effects of reducing carbon emissions, eliminating kiln drying, and saving energy costs
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Embodiment 1
[0429] Example 1: General method of soaking and germination
[0430] Dried barley kernels are placed in the aqueous solution in Plexiglass cylinders and are constantly ventilated with atmospheric air from below the kernel column. exist figure 1 and figure 2 A schematic of the equipment used is provided in . use 50 mass flow meters and controllers (Sierra, CA, USA) to set the airflow, and a Testo 735 precision thermometer (Testo, Germany) to measure the temperature.
[0431] For measuring airflow, temperature, pH, conductivity, redox potential and O of immersion water 2 Content sensors are integrated in the system. Sensors not only monitor the process in real time, but also adjust steeping and malting conditions during the process; this level of control is not possible with current malting and brewing protocols.
[0432] GA is a plant hormone that activates the aleurone layer in germinated barley. Many maltists add low concentrations of GA to the malting pr...
Embodiment 2
[0438] Example 2: Method for Determining Enzyme Activity
[0439] During germination, barley grains start to secrete a series of hydrolytic enzymes such as α-amylase, limit dextrinase and (1,3;1,4)-β-glucanase. Typically, these enzyme activities are detected in a time-coordinated manner, where the activity of alpha-amylase, beta-amylase and / or minimal dextrinase can be used as a general marker of hydrolase activity. Therefore, the activity of alpha-amylase and limit dextrinase is monitored by measuring the activity of hydrolytic enzymes in the grain and determined after germination according to the method of the present invention.
[0440] Sample Preparation
[0441] Germinated grains were milled using a standard Foss Cyclotech mill (Foss, Denmark) equipped with a tungsten carbide grinding ring (Foss 10004463), a nickel-plated impeller (Foss 10002666) and a 1 mm exit screen (Foss 10001989) prior to enzyme activity analysis sample. All measurements of enzyme activity...
Embodiment 3
[0448] Example 3: Grain development during soaking and germination procedures.
[0449] Hullless and hulled barley lines were incubated in Perspex cylinders according to the general procedure described in Example 1 . Incubation programs were performed at 15°C and 25°C. The grain is aerated with different levels of atmospheric air from below for different periods of time, during which time the grain moisture content rises and germination begins. Kernel development was analyzed after 24 and 48 hours of incubation and water uptake in the kernel was determined by measuring the water content in % (w / w).
[0450] In detail, kernels of hullless barley lines were transferred into plexiglass cylinders and incubated using the WA protocol. Grains were first incubated in water adjusted to 1000 nM gibberellic acid (GA) and 0.01% Foamazol FCD511 (AB Vickers, Burtonon Trent, UK) for 45 hours followed by 3 hours incubation in 1% P3-hypochloran (Ecolab, Switzerland). Incubation was pe...
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