DNA detection method based on solid-phase rolling circle amplification-nanogold-terahertz technology

A detection method, terahertz technology, applied in the field of terahertz spectrum detection, can solve problems such as annihilation, signal interference, target molecule detection signal interference, etc., achieve high application prospects and avoid signal interference

Pending Publication Date: 2020-08-18
SHENZHEN LUOHU PEOPLELS HOSPITAL
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  • Abstract
  • Description
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Problems solved by technology

A large number of non-target molecules will produce signal interference under the state of THz wave label detection, resulting in interference and annihilation of the detection signal of target molecules. Scientific problems that must be solved when technology is moving towards clinical practice

Method used

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  • DNA detection method based on solid-phase rolling circle amplification-nanogold-terahertz technology
  • DNA detection method based on solid-phase rolling circle amplification-nanogold-terahertz technology
  • DNA detection method based on solid-phase rolling circle amplification-nanogold-terahertz technology

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Experimental program
Comparison scheme
Effect test

Embodiment

[0050] Step 1: Making a THz Metamaterial Biosensor

[0051] Deposit a microprocessed gold film SRRs array on a silicon substrate, specific parameters: 1. Substrate silicon material: thickness 500 μm, unit area 15mm*15mm; 2. Gold film SRRs array: SRR: thickness 120nm, double opening circular, The opening gap (G) is 3 μm wide, the double opening angle (θ) is 120 degrees, the inner diameter of the ring is 48 μm, and the outer diameter is 60 μm; SRRs array: the center distance of the circle is 90 μm, and the array area is 5mm*5mm. Structural diagram see figure 1 .

[0052] Step 2: HBV DNA solid-phase rolling circle amplification (Rolling circle amplification, RCA)

[0053] 1. Oligonucleotide probe and primer design

[0054] The sequences of oligonucleotide probes and primers are shown in Table 1, and all oligonucleotide fragments were purified by high performance liquid chromatography (HPLC).

[0055] Table 1. Oligonucleotide probe and primer sequences

[0056]

[0057] 2....

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Abstract

The invention discloses a DNA detection method based on a solid-phase rolling circle amplification-nanogold-terahertz technology. According to the invention, gold magnetic nanoparticles are used as acarrier, and a solid-phase rolling circle amplification technology is utilized to realize first signal amplification of DNA detection; a local surface plasma resonance signal is enhanced by using a THz metamaterial to realize a second amplification effect on the DNA detection signal; a DNA amplification sample is modified by utilizing nanogold with high refractive index, and a third amplificationeffect of a detection signal is realized, so that high-sensitivity detection of DNA is realized on the basis of a triple signal amplification effect of target DNA detection. In addition, on one hand,a solid-phase roll-back amplification method is used for carrying out specific amplification on the target DNA; on the other hand, the gold magnetic nanoparticles are used for separating DNA amplification products to avoid signal interference of non-target molecules, so that high-specificity detection of DNA is realized on the basis of the two technologies.

Description

technical field [0001] The invention belongs to the technical field of terahertz spectrum detection, and relates to a DNA detection method based on solid-phase rolling circle amplification-nano gold-terahertz technology. Background technique [0002] Terahertz radiation (THz) waves are located between microwaves and infrared rays in the electromagnetic spectrum, and their frequency ranges from 0.1THz to 10THz (1THz=10 12 Hz, or 1THz=4.14meV photon energy), the wavelength range is 30μm~3mm. THz wave technology combines the characteristics of electronics and photonics. It is a typical interdisciplinary subject and has broad and broad application prospects in many fields such as biomedicine, quality control, security and defense, environmental monitoring, and aerospace. Its application in the field of biomedicine is giving birth to a new discipline, namely "terahertz - biomedicine". Laboratory medicine is an important branch in the field of biomedicine. The development of THz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3581C12Q1/6825C12Q1/682
CPCG01N21/3581C12Q1/6825C12Q1/682C12Q2531/125C12Q2563/137C12Q2563/155C12Q2563/143C12Q2565/607
Inventor 李育敏张阳张秀明
Owner SHENZHEN LUOHU PEOPLELS HOSPITAL
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