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Composition for regeneration of human fibrous cartilage or elastic cartilage

A technology for regenerating fibers and fibrocartilage. It is used in drug combinations, tissue regeneration, bone diseases, etc. It can solve problems such as incision of the knee joint, unproven stability and effectiveness, and achieve the effect of improving activities.

Pending Publication Date: 2020-08-25
BEF医疗公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the case of strut-based grafts, a polymer strut processed into an appropriate morphology is sutured to a partially resected cartilage plate, although it has been evaluated as an alternative to the above-mentioned allogeneic meniscal transplantation technique, but Stability and effectiveness have not been proven
In addition, in support-based transplantation, there is a problem that the knee joint must be incised until a specific form of support is inserted into the cartilage plate.

Method used

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  • Composition for regeneration of human fibrous cartilage or elastic cartilage
  • Composition for regeneration of human fibrous cartilage or elastic cartilage
  • Composition for regeneration of human fibrous cartilage or elastic cartilage

Examples

Experimental program
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preparation example Construction

[0049] Also, the present invention provides a method for preparing a composition for regenerating fibrocartilage or elastic cartilage, comprising: step 1), preparing 10 mg / ml to 1000 mg / ml of fibrinogen as the first solution; and step 2), mixing Thrombin and physiologically active polymers are used to prepare the second solution.

[0050] According to the preparation method of the present invention, it includes the step of preparing the preparation material for the hydrogel of regenerated fibrocartilage or elastic cartilage, and in the case that the individual who needs to regenerate fibrocartilage or elastic cartilage needs to perform treatment / operation, the hydrogel can be rapidly prepared Gel, through a minimally invasive method, injects hydrogel into the joint cavity. That is, the preparation method of the present invention may include: the preparation process of the first solution with fibrinogen as the main component and thrombin and physiologically active macromolecule...

Embodiment 1

[0067] Embodiment 1. is used to prepare the preparation of the first solution of hydrogel and the second solution

[0068] The regeneration of defective human tissue is realized only by minimally invasive method, and the first solution and the second solution as hydrogel preparation substances are prepared.

[0069] 1.1 Preparation of substances using hydrogel preparations of fibrin and polyethylene oxide

[0070]A first solution and a second solution of a preparation material for a hydrogel using fibrin and polyethylene oxide were prepared. A first solution was prepared by mixing 10 mg / ml to 1000 mg / ml of fibrinogen (Sigma-Aldrich) and 100-5500 KIU / ml of aprotinin (Sigma-Aldrich). A second solution was prepared by dissolving polyethylene oxide and calcium chloride in a solvent in which thrombin (Reyon Pharmaceuticals) powder was dissolved at a concentration of 100 Unit(U) / mL to 2500 Unit(U) / mL at a temperature of 37°C, Polyethylene oxide is prepared at a concentration of 1 ...

Embodiment 2

[0080] Example 2. Confirmation of compression and shear elastic modulus of hydrogel according to thrombin concentration

[0081] In order to confirm the change in the elastic modulus of the hydrogel generated according to the combination of the concentrations of fibrinogen and thrombin, the hydrogel was prepared in a mold with a diameter of 8.0 mm to measure the shear elastic modulus and the compressive elastic modulus. The shear modulus of elasticity was measured with a rheometer (ARES-LS, TA Instruments), the distance between the plates was set at 900 μm, and the vibration frequency was adjusted to 0.1 Hz and 100 Hz. The compressive modulus of elasticity was measured using a universal tensile testing machine (Instron 5966, Instron Corporation). In the stress-deformation rate graph, the compressive modulus of elasticity (E) value is derived using the slope of the interval with the elastic interval. The concentration of fibrinogen was fixed at a constant concentration of 100 ...

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PUM

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Abstract

The present invention relates to a composition and a kit for regeneration and treatment of cartilage, preferably, fibrous cartilage or elastic cartilage and a regeneration method using the same. A composition and a kit for cartilage regeneration according to the present invention can be administered simply in a minimally invasive manner to a site in which fibrous cartilage or elastic cartilage isneeded to be regenerated or restored from injury, exhibit resistance to degradation enzymes without toxicity within the body, and are attached to or detained at and thus retained at the injured, administered site, whereby behavioral improvement can be brought about in surrounding cells, which leads to effectively inducing defected tissues of the meniscus to be regenerated. Therefore, the composition of the present invention is useful as a mediator for aiding the regeneration of biological tissue defected regions in the biomaterials field.

Description

technical field [0001] The present invention relates to compositions, kits and regeneration methods using same for regeneration and treatment of fibrocartilage or elastic cartilage. Background technique [0002] Like other connective tissues, cartilage is composed of connective tissue cells and an extracellular matrix, but, unlike connective tissue propria, is a specialized connective tissue comprising a matrix that is hard and somewhat flexible. Cartilage is classified into hyaline cartilage, elastic cartilage, and fibrous cartilage according to the type and characteristics of fibers forming the cartilage matrix. Hyaline cartilage mainly contains type II collagen and is the most common type of cartilage. Most of the type II collagen is shown in the form of microfibrils, and since the refractive index (refractory index) is almost the same as that of protein sugar, which is the main component of the amorphous substance, it is observed that the fiber component and the amorpho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/36A61K38/48A61K9/06A61P19/00
CPCA61K38/48A61P19/00A61K38/363A61L27/26A61L27/52A61L27/50A61L27/3616A61L2400/06A61L2430/06C08L89/00C08L71/02A61K38/4833A61K9/06A61L27/18A61L27/225A61L27/3654A61L27/3687A61L27/54A61L2300/254
Inventor 安承昱
Owner BEF医疗公司
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