Self-assembly material capable of forming nano defense network in situ on tumor as well as preparation method and application of self-assembly material

A self-assembly, in-situ technology, applied in the field of biomedicine, to achieve real-time bioimaging, improve biosafety and bioavailability, good biocompatibility and mechanical properties

Active Publication Date: 2020-09-04
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But at present, there are still relatively few therapeutic strategies for inhibiting tumor metastasis in the prior art. Therefore, it is very important to develop a new type of therapeutic strategy that can inhibit tumor metastasis with significant curative effect

Method used

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  • Self-assembly material capable of forming nano defense network in situ on tumor as well as preparation method and application of self-assembly material
  • Self-assembly material capable of forming nano defense network in situ on tumor as well as preparation method and application of self-assembly material
  • Self-assembly material capable of forming nano defense network in situ on tumor as well as preparation method and application of self-assembly material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] This example constructs a self-assembling material that can form a nano-defense network in situ on a tumor. The self-assembling material is composed of a targeting peptide, a self-assembling polypeptide and a bispyrene fluorescent signal molecule. Its chemical structure is as follows;

[0077]

[0078] (1) Swell Wang resin with N,N-dimethylformamide;

[0079] (2) Using the terminal amino group to obtain Fmoc protection, the amino acid of the side chain amino group to obtain Boc protection and the bispyrene fluorescent signal molecule as raw materials, first, according to the amino acid sequence of Ala-Thr-Trp-Leu-Pro-Pro-Arg, add Arg To the carrier resin, carry out coupling reaction and connection with Wang resin; take off the Fmoc protecting group on Arg, and carry out coupling reaction and connection of the second amino acid Pro with Arg; until the completion of Ala-Thr-Trp-Leu-Pro- Condensation of all amino acids in Pro-Arg;

[0080] (3) Remove the Fmoc protectin...

Embodiment 2

[0085] This embodiment prepares self-assembled nanoparticle solution and nanofiber dispersion, the specific method is as follows:

[0086] The self-assembled material that embodiment 1 is made is dissolved in DMSO solvent (the concentration of self-assembled material is 1.5 * 10 -3 M), take the above solution and place it in a centrifuge tube, then slowly add deionized water into the centrifuge tube to prepare mixed solutions with different water contents (0%, 40%, 98%), and mix the self-assembly material monomer solution ( Water content 0%), self-assembled nanoparticle solution (water content 40%) and self-assembled nanoparticle solution (water content 98%), carry out fluorescence detection with fluorescence spectrophotometer respectively, and fluorescence spectrum is as follows figure 2 shown by figure 2 It can be seen that both the self-assembled nanoparticle solution (40% water content) and the self-assembled nanoparticle solution (98% water content) have a peak fluores...

Embodiment 3

[0091] SEM test:

[0092]In this example, human-derived breast cancer cells MDA-MB-231 were used as the cell model, and a silicon chip was placed at the bottom of the culture dish. Cultivate in DMEM medium for 24 hours, then add 30 μM self-assembled material prepared in Example 1 and cultivate for 2 hours, the cultivation temperature is 37.0°C, CO 2 At a concentration of 5.0%, cells were grown on silicon wafers.

[0093] Then the medium was discarded, washed three times with PBS, fixed with 4% paraformaldehyde for 1 h, washed twice with PBS, and then treated with 30%, 50%, 70%, 90%, and 100% ethanol / PBS solutions. Gradient dehydration of cells, each concentration was dehydrated twice, 10 min each time, and finally the cells were rinsed with tert-butanol for 30 min. After the treatment, the cells were dried in a vacuum drying oven, and observed and scanned with a scanning electron microscope after drying. Test results such as Figure 5 As shown, it can be seen from the figu...

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Abstract

The invention relates to a self-assembled material capable of forming a nano defense network in situ on a tumor as well as a preparation method and an application of the self-assembled material, the self-assembled material is composed of a targeting peptide, a self-assembled polypeptide and a dipyrene fluorescence signal molecule, and the chemical structure of the self-assembled material is shownas a formula (I); R1 is from a self-assembled polypeptide with multiple hydrogen bonds in a molecule; and R2 is from a tumor targeting peptide. The preparation method comprises the step of synthesizing the self-assembled material by taking amino acid with protected terminal amino and side chain amino and a dipyrene fluorescence signal molecule as raw materials through a solid-phase synthesis method. The tumor site can be actively targeted; a tumor part can be induced to deform; according to the preparation method, a nanofiber network structure is formed through self-assembly, namely a nano defense network can be formed in situ in a tumor, so that tumor cells are released to induce capture of vascular endothelial growth factors generated by new blood vessels, generation of new blood vesselsis prevented, nutrient supply of the tumor is cut off, and meanwhile, a metastasis path of the tumor is also blocked.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a self-assembly material and its preparation method and application, in particular to a self-assembly material capable of forming a nanometer defense network in situ on a tumor, its preparation method and its application. Background technique [0002] Tumor refers to the new organism formed by the proliferation of local tissue cells under the action of various tumorigenic factors. Tumors are divided into benign tumors and malignant tumors. Malignant tumors seriously endanger people's health. The reason for high cancer mortality is mainly due to rapid tumor metastasis. Therefore, inhibiting tumor metastasis is a key factor in tumor treatment. Tumor metastasis is an extremely complex process, among which, the inhibition of tumor metastasis by inhibiting the activity of matrix metalloproteinases is a common strategy, because matrix metalloproteinases degrade the extracellular matrix is ​​...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06A61K31/4025A61K31/4178A61K31/40C07K7/08C07K14/00C07K1/06C07K1/04A61P35/00
CPCC09K11/06A61K31/4025A61K31/4178A61K31/40C07K7/08C07K14/00A61P35/00C09K2211/1011Y02P20/55
Inventor 王浩王磊李秉南
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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