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Preparation method of acrylic resin coated recombinant human auxin microcapsule

An acrylic resin and auxin technology, applied in microcapsules, capsule delivery, medical preparations with non-active ingredients, etc., can solve the problems of difficulty in improving the encapsulation rate and uneven distribution of drugs, and achieve uniform and controllable particle size. The effect of high encapsulation rate and low burst release rate

Inactive Publication Date: 2020-09-11
上海舒泽生物科技研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inhomogeneous particle size leads to uneven distribution of drugs in small particle size microcapsules, and it is difficult to improve the embedding rate. Among them, the application of larger particle sizes is also limited by the route of administration.

Method used

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  • Preparation method of acrylic resin coated recombinant human auxin microcapsule
  • Preparation method of acrylic resin coated recombinant human auxin microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: as figure 1 , a preparation method of acrylic resin-coated recombinant human auxin microcapsules, the specific steps are:

[0034] S1, add 100g of methacrylic acid, 82g of methyl acrylate and 1.1g of ammonium persulfate into 500mL of chloroform, heat up to 80°C, react for 1h and then cool to room temperature to form a film material solution;

[0035] S2. Weigh the recombinant human auxin raw material drug with a potency of 1.5 mg / amp and 4.0 IU / amp, and use phosphate buffer solution with pH=7.4 to prepare it into a drug solution of 50 mL and 8 mg / L;

[0036] S3, adding the drug solution obtained in step S2 to the membrane material solution in step S1, stirring at 300 rpm for 3 minutes, and pressing through the microporous membrane device at a pressure of 2000 kPa to obtain a mixed solution;

[0037] S4, chloroform was removed by rotary evaporation at 30° C. at a speed of 12000 rpm. Under the condition of 4°C, the uncoated recombinant human auxin was remo...

Embodiment 2

[0038] Embodiment 2: A kind of preparation method of acrylic resin coated recombinant human auxin microcapsules, the specific steps are:

[0039] S1, add 200g of methacrylic acid, 165g of methyl acrylate and 2.4g of ammonium persulfate into 1000mL of chloroform, raise the temperature to 80°C, react for 1h and then cool to room temperature to form a membrane material solution;

[0040] S2. Weigh the recombinant human auxin raw material drug with a potency of 1.5 mg / amp and 4.0 IU / amp, and use phosphate buffer solution with pH=7.4 to prepare it into a drug solution of 90 mL and 10 mg / L;

[0041] S3, adding the drug solution obtained in step S2 to the membrane material solution in step S1, stirring at 300 rpm for 3 minutes, and pressing through the microporous membrane device at a pressure of 2000 kPa to obtain a mixed solution;

[0042] S4, chloroform was removed by rotary evaporation at 30° C. at a speed of 12000 rpm. Under the condition of 4°C, the uncoated recombinant human ...

Embodiment 3

[0043] Embodiment 3: Embodiment 2: a kind of preparation method of acrylic resin coated recombinant human auxin microcapsules, the specific steps are:

[0044] S1, add 100g of methacrylic acid, 82g of methyl acrylate and 1.1g of potassium persulfate into 500mL of chloroform, heat up to 80°C, react for 1h and then cool to room temperature to form a film material solution;

[0045] S2. Weigh the recombinant human auxin raw material drug with a potency of 1.5 mg / amp and 4.0 IU / amp, and use phosphate buffer solution with pH=7.4 to prepare it into a drug solution of 50 mL and 10 mg / L;

[0046] S3, adding the drug solution obtained in step S2 to the membrane material solution in step S1, stirring at 300 rpm for 3 minutes, and pressing through the microporous membrane device at a pressure of 2000 kPa to obtain a mixed solution;

[0047] S4, chloroform was removed by rotary evaporation at 30° C. at a speed of 12000 rpm. Under the condition of 4°C, the uncoated recombinant human auxin...

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Abstract

The invention discloses a preparation method of an acrylic resin coated recombinant human auxin microcapsule, and relates to the technical field of medicine preparation. The preparation method comprises the following specific steps of S1, adding methacrylic acid, methyl acrylate and an initiator into chloroform to form a membrane material solution; S2, weighing a recombinant human auxin bulk drug,and preparing the recombinant human auxin bulk drug into 8-10 mg / L of a drug solution by adopting a phosphate buffer solution with the pH value of 7.4; S3, adding the drug solution into a membrane material solution, performing stirring, and passing through a microporous membrane under a certain pressure to obtain a mixed solution; and S4, removing the chloroform in the mixed solution, and performing centrifuging and freeze-drying to obtain the acrylic resin coated recombinant human auxin microcapsule. The acrylic resin coated recombinant human auxin microcapsule obtained by the preparation method has the advantages of high embedding rate, high biological activity and good stability.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, more specifically, it relates to a preparation method of acrylic resin-coated recombinant human auxin microcapsules. Background technique [0002] In 1920, people discovered the existence of growth hormone (human growth hormone, hGH). Growth hormone is a protein secreted by adenohypophysis cells. pause. There are 4 α-helices in the molecular structure of growth hormone, so that growth hormone can be well combined with its receptor. In 1958, it was discovered that human growth hormone could be used to treat dwarfism. However, at that time, human growth hormone could only be extracted from the pituitary gland of cadavers, and the quantity was limited, so it was difficult to guarantee the clinical dosage, thus limiting its application. In 1986, Boyer successfully synthesized recombinant human growth hormone (rhGH) through gene recombination technology. Its structure is exactly the sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/32A61K38/27A61P5/06A61P3/00
CPCA61K9/5026A61K38/27A61P5/06A61P3/00
Inventor 楼秀余
Owner 上海舒泽生物科技研究所
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