A kind of valve material with synergistic anticoagulation and anticalcification functions and preparation method thereof

A valve and functional technology, applied in the field of valve materials with synergistic anticoagulant and anticalcification functions and its preparation, can solve problems such as easy calcification and thrombus formation, and achieve improved service life, good antithrombotic activity, and improved anticoagulant effect Effect

Active Publication Date: 2021-03-30
JILIN VENUS HAOYUE MEDICAL LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention provides a valve material with synergistic anticoagulant and anti-calcification functions and a preparation method thereof, which can effectively solve the problem of remaining aldehyde groups in the valve material prepared by the existing method. The problems of easy calcification and thrombosis caused by this method can be used as valve materials for aortic valve, pulmonary valve, venous valve, mitral valve and tricuspid valve replacement.

Method used

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  • A kind of valve material with synergistic anticoagulation and anticalcification functions and preparation method thereof
  • A kind of valve material with synergistic anticoagulation and anticalcification functions and preparation method thereof
  • A kind of valve material with synergistic anticoagulation and anticalcification functions and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0031] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:

[0032] (1) Cut the degreased pig pericardium material into a suitable size, stretch it and immerse it in a glutaraldehyde solution with a concentration of 1% and a pH value of 7, change the solution for 24 hours, and continue the treatment for 72 hours;

[0033] (2) Immerse the valve material treated in step (1) into a sealing solution (pH 7.4) containing polyethyleneimine (10mg / mL), treat it at room temperature for 0.5h, and seal the residual glutaraldehyde after cross-linking. The aldehyde group;

[0034] (3) Transfer the glutaraldehyde valve obtained in the above steps to MES buffer, add heparin (5mg / mL) and N,N'-dicyclohexylcarbodiimide (0.5mg / mL), and add Under these conditions, cross-linking treatment at 4°C for 24 hours;

[0035] (4) After washing the valve material obtained after the above reaction liquid treatment, ...

Embodiment 2

[0037] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:

[0038] (1) Cut the pig pericardium material after degreasing to a suitable size, immerse it in a glutaraldehyde solution with a concentration of 0.5% and a pH value of 8.5 for 24 hours after being stretched, and then transfer to a 1% glutaraldehyde solution Medium treatment for 48 hours;

[0039] (2) Immerse the valve material treated in step (1) into a sealing solution (pH7.4) containing polylysine (5 mg / mL), treat it at room temperature for 6 hours, and seal the glutaraldehyde cross-linked residual The aldehyde group;

[0040] (3) Transfer the glutaraldehyde valve obtained in the above steps to PBS buffer, add bivalirutin (1 mg / mL) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide salt salt (0.3mg / mL), under the condition of pH6, cross-linking treatment at 25°C for 4h;

[0041] (4) After washing the valve material obtained...

Embodiment 3

[0043] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:

[0044] (1) The bovine pericardium material after degreasing is cut into suitable size, after being stretched, immerse into the glutaraldehyde solution with a concentration of 1% and a pH value of 6.5 for 24 hours, and then transfer to 0.5% glutaraldehyde solution Medium treatment for 48 hours;

[0045] (2) Immerse the valve material after the treatment in step (1) into a sealing solution (pH 7.4) containing hexamethylenediamine (4 mg / mL), and treat it at room temperature for 3 hours to seal the residual aldehyde group after glutaraldehyde crosslinking group;

[0046] (3) Transfer the glutaraldehyde valve obtained in the above steps to PBS buffer, add hirudin (2 mg / mL) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride ) (0.4mg / mL), under the condition of pH5, cross-linking treatment at 37°C for 2h;

[0047] (4) ...

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Abstract

The invention provides a valve material with synergistic anticoagulant and anti-calcification functions and a preparation method thereof. The preparation method comprises the following steps: performing glutaraldehyde cross-linking treatment on animal source biological valve material; immersing the treated valve material in Treat in the blocking solution containing amine compounds for 0.5-6h, and then seal the residual aldehyde groups after glutaraldehyde cross-linking; then put the valve material into the reaction solution containing anticoagulant and cross-linking agent, at 4℃- Cross-linking treatment at 37° C. for 6-24 hours; finally, the valve material is washed to prepare the valve material, and the valve material is stored in a mixed solvent of glutaraldehyde or isopropanol / glycerol. This method can effectively solve the problems of easy calcification and thrombus formation caused by aldehyde residues in the valve materials obtained by the existing method, and the valve materials prepared according to this method can be used for aortic valves, pulmonary valves, and venous valves. , Mitral and tricuspid valve replacement valve materials required.

Description

technical field [0001] The invention belongs to the technical field of modification of biomedical materials and medical devices, and specifically relates to a valve material with synergistic anticoagulant and anti-calcification functions and a preparation method thereof. The valve material prepared according to the method can be used for aortic valves, Valve material for pulmonary, venous, mitral and tricuspid valve replacements. Background technique [0002] Transcatheter interventional valve therapy is becoming the mainstream direction of valve replacement, and the technologies currently developed relatively include aortic valve, pulmonary valve, mitral valve, tricuspid valve and venous valve replacement. At present, the mainstream valve materials used in interventional valve surgery are heterogeneous biological valve materials, mainly derived from porcine pericardium, bovine pericardium, small intestinal submucosa and swim bladder. By chemically cross-linking animal-deri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/54A61L33/00A61L33/10A61L33/12
CPCA61L27/3625A61L27/3604A61L27/3629A61L27/3641A61L27/3687A61L27/54A61L33/0041A61L33/08A61L33/128A61L2430/20A61L2430/40A61L2400/18A61L2300/232A61L2300/252A61L2300/42C08L5/10C08L89/00A61L27/507A61L2400/02A61L33/0011A61L33/0082A61L2300/236A61L2300/418C12N5/0697
Inventor 王云兵杨立罗日方李林华
Owner JILIN VENUS HAOYUE MEDICAL LTD
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