Preparation method of chitosan/sodium alginate dual-network hydrogel

A sodium alginate and chitosan technology, applied in biochemical equipment and methods, bone/connective tissue cells, cell culture support/coating, etc., can solve problems such as negative effects, and achieve low cytotoxicity and simple operation , Improve the effect of mechanical properties

Active Publication Date: 2020-09-15
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the modification of most chitosan requires the use of cross-linking agents, such as glutaraldehyde, and cross-linking agents are generally toxic, which has a great negative impact on subsequent applications

Method used

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  • Preparation method of chitosan/sodium alginate dual-network hydrogel
  • Preparation method of chitosan/sodium alginate dual-network hydrogel
  • Preparation method of chitosan/sodium alginate dual-network hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) 5g of chitosan was slowly added to 50mL of 50wt% NaOH aqueous solution prepared in advance, and placed at -20°C for 24h. Then, chitosan was thawed and dispersed in 150–200 mL of isopropanol to form a homogeneous solution. To this solution was added 8.5 g of chloroacetic acid and stirred at room temperature for 5 h (25 °C, 1500 rpm). The product was purified by dialysis against deionized water (Mw=3500) for 3 days, and then freeze-dried to obtain carboxymethyl chitosan (CMC). Then 1 g of carboxymethyl chitosan, 0.1 g of transglutaminase and 1 g of collagen peptide were dissolved in 30 mL of PBS (0.2 mol / L, pH=6.0) solution and mixed in a flask, and reacted at 40 ° C for 4 h (300rpm), keep in boiling water for 10min. After cooling to room temperature (25° C.), the product was dialyzed against deionized water (Mw=3500) for 4 days, and then freeze-dried to obtain carboxymethyl chitosan-collagen peptide (CMC-COP).

[0031] (2) First, 5 g of sodium alginate (SA) was un...

Embodiment 2

[0036] (1) the CMC-COP prepared in the embodiment 1, the OSA prepared in the embodiment 1 are dissolved in water, the pre-polymerization solution made, the mass percent of CMC-COP is 7.5%, the mass percent of OSA is 20%, The single network hydrogel OSA / CMC-COP was obtained by reacting at room temperature (25° C.) for 3 min in a glass sample vial.

[0037] (2) the CMC-COP prepared in Example 1, the OMSA prepared in Example 1 and the I2959 photoinitiator are dissolved in water, and the pre-poly solution made, the mass percent of CMC-COP is 7.5%, the mass percent of OSA The percentage is 20%, the mass percentage of I2959 photoinitiator is 0.1%, and reacted in a glass sample bottle at room temperature (25° C.) for 3 minutes to obtain a single-network hydrogel. Then place the single-network hydrogel at a wavelength of 365nm and a power of 1mW / cm 2 The photocrosslinking occurred after irradiating with ultraviolet light for 15min to obtain the double network hydrogel OMSA / CMC-COP. ...

Embodiment 3

[0039] Example 3: The OSA / CMC-COP single network hydrogel and OMSA / CMC-COP double network hydrogel prepared in Example 2 were applied to this example.

[0040] Put the prepared hydrogel into a freeze dryer, freeze-dry at -56°C for 12h, and then take it out. After spraying a thin layer of gold on the freeze-dried CMC-COP / OSA and CMC-COP / OMSA samples, the internal structure of the freeze-dried hydrogel was photographed by scanning electron microscopy. image 3 The internal microscopic morphology of the hydrogel is characterized by scanning electron microscopy. The hydrogel has a porous structure and is the preferred material for wound dressings. Its pore size is conducive to storing water, keeping the wound moist and breathable, and providing a good environment for cell growth. . image 3 (Left) is the internal microscopic morphology of OSA / CMC-COP single-network hydrogel after freeze-drying, with large pore size and different sizes. image 3 (Right) The right is the internal ...

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Abstract

The invention discloses a preparation method of chitosan/sodium alginate dual-network hydrogel, comprising the following steps of: carrying out a Schiff base reaction on a prepolymer solution composedof modified chitosan, modified sodium alginate, a photoinitiator and water to obtain single-network hydrogel; and reacting the single-network hydrogel under the irradiation of ultraviolet light to obtain secondary photo-crosslinked chitosan/sodium alginate double-network hydrogel, wherein the modified chitosan is carboxymethyl chitosan grafted with a collagen peptide; the modified sodium alginateis formylated sodium alginate modified by methacrylic acid. The collagen peptide disclosed by the invention has non-immunogenicity, and can be grafted on chitosan through an enzymatic reaction underthe condition of not using a toxic cross-linking agent, so that the cell compatibility and the cell proliferation promoting capability of the material are improved.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a preparation method of a chitosan / sodium alginate double network hydrogel. Background technique [0002] The skin is on the surface of the body, directly in contact with the external environment, and has functions such as protection, excretion, temperature regulation, and perception of external stimuli. It is the largest organ of the human body. However, a large area of ​​the skin is exposed, and it is extremely vulnerable to various injuries in daily life, such as mechanical trauma, burns, and chronic ulcers. Skin wound healing is a major clinical problem, and wound infection caused by bacteria during wound recovery is the most common complication, and severe cases can lead to death. Due to its natural porous structure and high water content, hydrogel can absorb a large amount of exudate, act as a barrier against bacteria, and also maintain a moist env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08J3/28C08J9/28C08L5/04C08L87/00C08L5/08A61L15/28A61L15/32A61L15/42C12N5/077
CPCC08J3/075C08J3/246C08J3/28C08J9/28A61L15/28A61L15/325A61L15/42A61L15/425C12N5/0656C08J2305/04C08J2487/00C08J2405/08C08J2201/0484C12N2533/54C12N2533/74C12N2533/72C08L5/08C08L5/04
Inventor 毛宏理顾忠伟何雨芯
Owner NANJING UNIV OF TECH
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