Kit for detecting 15 bile acids in serum and application of kit

Abstract

The invention discloses a kit for detecting 15 bile acids in serum and an application of the kit, and belongs to the technical field of blood detection. The 15 bile acids comprise a cholic acid, a glycocholic acid, a taurocholic acid, a lithocholic acid, a glycolithocholic acid, a taurolithocholic acid, a deoxycholic acid, a glycodeoxycholic acid, a taurodeoxycholic acid, a chenodeoxycholic acid,a glycochenodeoxycholic acid, a taurochenodeoxycholic acid, an ursodeoxycholic acid, a glycoursodeoxycholic acid and a tauroursodeoxycholic acid. The kit comprises an eluent, a calibration product solution, a mixed internal standard working solution, a protein precipitant and a quality control product. When the kit is used for detecting the bile acids in the serum, a to-be-detected sample does notneed derivatization treatment, pretreatment is simple, a sample dosage is small, sensitivity is high, specificity is strong, more types can be detected, the 15 types of bile acid can be simultaneously detected within 6.5 minutes, and the kit can be used for clinical diagnosis and health assessment of serum bile acids.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a kit for detecting 15 kinds of bile acids in serum by using ultra-high performance liquid chromatography tandem mass spectrometry technology and an application thereof. Background technique [0002] Bile acid is the main component of bile. It is a general term for a class of 24-carbon cholic acid hydroxyl derivatives. It is the final product of cholesterol metabolized by liver tissue. When the liver circulation is destroyed, bile acid cannot be reused. Bile acids can be divided into free bile acids and conjugated bile acids according to their structure. Free bile acids in the human body can be divided into primary and secondary bile acids according to their production sites and structures. Primary bile acids are converted from intrahepatic cholesterol, and through a series of reactions, finally generate cholic acid (CA) and chenodeoxycholic acid (Chenodeoxyc...

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Problems solved by technology

CN 106841492 A discloses a method for detecting five kinds of free bile acids in serum by high performance liquid chromatography tandem mass spectrometry, but this method has certain defects, such as only detecting five kinds of free bile acids, which has limited significance for disease diagnosis and clinical , after the pretreatment protein precipitation, the supernatant needs to be reconstituted after being lyophilized by a centrifugal concentration and freezing system. The pretreatment method is complicated, and the detection time on the machine is as long as 12.5 minutes, which is difficult to apply in clinical detection

CN 107356694 A discloses a method for efficiently detecting 15 kinds of bile acids in blood. Although the method can detect 15 kinds of bile acids at the same time, it also has certain defects. Filter and air-dry for more than 3 hours, punch a sample on the obtained dried blood film, and then extract it through liquid-liquid extraction and nitro

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