Composite artificial skin material for repairing skin injury
A technology of artificial skin and keratin, applied in the field of skin repair, can solve the problems of skin defects, high price of composite membrane products, insufficient source of autologous skin, etc., to repair wound damage, have good antibacterial and repair effects, and maintain no infection Effect
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Embodiment 1
[0042] Embodiment 1: antibacterial film preparation
[0043] 1. Preparation of genipin cross-linked nisin / CS / fibrin antibacterial film
[0044] Prepare 5g / 100mL fibrinogen aqueous solution and 5g / 100mL CS (chitosan) solution respectively, inject 5mL fibrinogen aqueous solution into 6-well culture plate, then add 0.5mL CS solution and mix well, after a few seconds It can be seen that the milky white flocs are evenly distributed and stop immediately. 200 μl of thrombin (50 u / mL) was added dropwise, and after 5 minutes, 200 μl of 10 mg / mL genipin solution was evenly added dropwise, and 400 μl of 10 mg / mL nisin (nisin) was added. Put the culture plate in a 37°C drying oven for 12 hours, and the liquid gradually solidified into a light blue gel. Pressurize the gel with a weight (diameter 3.2 cm, mass 200 g) coated with glycerin on the bottom surface, and continue to solidify the gel until the gel is solidified. Before taking out the weight, add glycerol dropwise in the 6-well cu...
Embodiment 2
[0050] Embodiment 2: Observation of the morphology and structure of the antibacterial film
[0051] Structural observation is carried out to the antibacterial film prepared in embodiment 1:
[0052] Such as figure 1 As shown, using nisin, CS and fibrin as raw materials, the composite antibacterial film prepared by the cross-linking of genipin was dark blue in appearance and had strong elasticity and toughness. The blending of protein materials in the preparation of CS membranes can significantly improve the mechanical properties of the membranes, making the composite membranes meet the needs of practical applications.
[0053] from figure 2 It can be seen that the composite antibacterial membrane has a porous network structure with a large number of pores, uniform distribution of pores, small pore size, and high mechanical strength.
[0054] On the basis of the above antibacterial film, growth factors and EMSCs were implanted to prepare a composite antibacterial film. im...
Embodiment 3
[0055] Example 3: Establishment of animal full-thickness skin defect model and skin transplantation
[0056] Experimental rats aged 4-5 weeks were divided into 5 groups, 10 rats in each group, weighing about 250 g, marked with ear marks, and anesthetized by intraperitoneal injection.
[0057] Animal models of 2cm×2cm full-thickness skin defects were made on the back of rats, respectively, the non-implanted membrane material control group (group No. 1), the wound surface was only covered with vaseline oil gauze, and healed naturally; the ordinary membrane material group (group No. 2), Ordinary membrane materials without nisin, growth factors and EMSCs for wound transplantation; composite antibacterial membrane group (group 3), composite antibacterial membranes with nisin, growth factors and EMSCs for wound transplantation. The transplanted membrane material was sutured with 3-0 silk thread, fixed on the wound surface, and long thread was left. Rats in the three groups were rais...
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